ID   SPC25_DROPS             Reviewed;         235 AA.
AC   Q299P8;
DT   23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT   14-OCT-2008, sequence version 2.
DT   25-MAY-2022, entry version 55.
DE   RecName: Full=Kinetochore protein Spc25 {ECO:0000250|UniProtKB:Q9V3V7};
GN   Name=Spc25 {ECO:0000250|UniProtKB:Q9V3V7}; ORFNames=GA20206;
OS   Drosophila pseudoobscura pseudoobscura (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=46245;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MV2-25 / Tucson 14011-0121.94;
RX   PubMed=15632085; DOI=10.1101/gr.3059305;
RA   Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S.,
RA   Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O.,
RA   Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F.,
RA   Howells S.L., Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A.,
RA   Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M.,
RA   Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J.,
RA   Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y.,
RA   Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F.,
RA   Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M.,
RA   Weinstock G.M., Gibbs R.A.;
RT   "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal,
RT   gene, and cis-element evolution.";
RL   Genome Res. 15:1-18(2005).
CC   -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC       Ndc80 complex, which is required for chromosome segregation and spindle
CC       checkpoint activity during meiosis and mitosis. Required for
CC       kinetochore integrity and the organization of stable microtubule
CC       binding sites in the outer plate of the kinetochore. Participates in
CC       SAC signaling that responds specifically to disruptions in spindle
CC       microtubule dynamics. The NDC80 complex synergistically enhances the
CC       affinity of the SKA1 complex for microtubules and may allow the NDC80
CC       complex to track depolymerizing microtubules.
CC       {ECO:0000250|UniProtKB:Q9HBM1, ECO:0000250|UniProtKB:Q9V3V7}.
CC   -!- SUBUNIT: Component of the Ndc80 complex, which is composed of Ndc80,
CC       Nuf2 and Spc25. {ECO:0000250|UniProtKB:Q9V3V7}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9V3V7}.
CC       Chromosome, centromere, kinetochore {ECO:0000250|UniProtKB:Q9V3V7}.
CC   -!- SIMILARITY: Belongs to the SPC25 family. {ECO:0000255}.
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DR   EMBL; CM000070; EAL27655.4; -; Genomic_DNA.
DR   AlphaFoldDB; Q299P8; -.
DR   SMR; Q299P8; -.
DR   STRING; 7237.FBpp0292648; -.
DR   eggNOG; ENOG502RVT8; Eukaryota.
DR   InParanoid; Q299P8; -.
DR   Proteomes; UP000001819; Genome assembly.
DR   GO; GO:0031262; C:Ndc80 complex; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0051311; P:meiotic metaphase plate congression; ISS:UniProtKB.
DR   GO; GO:0000212; P:meiotic spindle organization; ISS:UniProtKB.
DR   GO; GO:0007080; P:mitotic metaphase plate congression; ISS:UniProtKB.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Centromere; Chromosome; Coiled coil;
KW   Kinetochore; Meiosis; Mitosis; Nucleus; Reference proteome.
FT   CHAIN           1..235
FT                   /note="Kinetochore protein Spc25"
FT                   /id="PRO_0000392424"
FT   REGION          193..216
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          44..106
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        196..216
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   235 AA;  26565 MW;  69766CDCC2395881 CRC64;
     MTDLKASEGD LSDYYMRLKK IFSNETRLQS REASISKRSS RVHKNILSAK EAIERQERDF
     GKLQKVLLNR NQELERRFTL GEALAQQLEV TRQRNADMEA QLLRHTTEGR QRSNELMECM
     HSLKQATGTY INHEAFPARL NGVSVVRADD GDIKLIPFSL DGNDADGLHT LWRSLHTRTD
     NASKWRKLIS DQEVAGASPV TPSGSERPKA TSKHSNFMPT SIIEIDLTSP TNDAS