ID   SPC25_DROSE             Reviewed;         222 AA.
AC   B4HGE0; Q64EW5;
DT   23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   25-MAY-2022, entry version 43.
DE   RecName: Full=Kinetochore protein Spc25 {ECO:0000250|UniProtKB:Q9V3V7};
GN   Name=Spc25 {ECO:0000250|UniProtKB:Q9V3V7};
GN   Synonyms=mitch {ECO:0000312|EMBL:EDW42388.1}; ORFNames=GM24067;
OS   Drosophila sechellia (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7238;
RN   [1] {ECO:0000312|EMBL:AAU15002.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=17895365; DOI=10.1242/jcs.012112;
RA   Williams B., Leung G., Maiato H., Wong A., Li Z., Williams E.V.,
RA   Kirkpatrick C., Aquadro C.F., Rieder C.L., Goldberg M.L.;
RT   "Mitch a rapidly evolving component of the Ndc80 kinetochore complex
RT   required for correct chromosome segregation in Drosophila.";
RL   J. Cell Sci. 120:3522-3533(2007).
RN   [2] {ECO:0000312|EMBL:EDW42388.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Rob3c / Tucson 14021-0248.25 {ECO:0000312|EMBL:EDW42388.1};
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC       Ndc80 complex, which is required for chromosome segregation and spindle
CC       checkpoint activity during meiosis and mitosis. Required for
CC       kinetochore integrity and the organization of stable microtubule
CC       binding sites in the outer plate of the kinetochore. Participates in
CC       SAC signaling that responds specifically to disruptions in spindle
CC       microtubule dynamics. The NDC80 complex synergistically enhances the
CC       affinity of the SKA1 complex for microtubules and may allow the NDC80
CC       complex to track depolymerizing microtubules.
CC       {ECO:0000250|UniProtKB:Q9HBM1, ECO:0000250|UniProtKB:Q9V3V7}.
CC   -!- SUBUNIT: Component of the Ndc80 complex, which is composed of Ndc80,
CC       Nuf2 and Spc25. {ECO:0000250|UniProtKB:Q9V3V7}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9V3V7}.
CC       Chromosome, centromere, kinetochore {ECO:0000250|UniProtKB:Q9V3V7}.
CC   -!- SIMILARITY: Belongs to the SPC25 family. {ECO:0000255}.
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DR   EMBL; AY714308; AAU15002.1; -; Genomic_DNA.
DR   EMBL; CH480815; EDW42388.1; -; Genomic_DNA.
DR   RefSeq; XP_002031402.1; XM_002031366.1.
DR   AlphaFoldDB; B4HGE0; -.
DR   SMR; B4HGE0; -.
DR   STRING; 7238.B4HGE0; -.
DR   EnsemblMetazoa; FBtr0207052; FBpp0205544; FBgn0082413.
DR   HOGENOM; CLU_1246541_0_0_1; -.
DR   OMA; SNELMEC; -.
DR   PhylomeDB; B4HGE0; -.
DR   Proteomes; UP000001292; Unassembled WGS sequence.
DR   GO; GO:0031262; C:Ndc80 complex; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0051311; P:meiotic metaphase plate congression; ISS:UniProtKB.
DR   GO; GO:0000212; P:meiotic spindle organization; ISS:UniProtKB.
DR   GO; GO:0007080; P:mitotic metaphase plate congression; IEA:EnsemblMetazoa.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Centromere; Chromosome; Coiled coil;
KW   Kinetochore; Meiosis; Mitosis; Nucleus; Reference proteome.
FT   CHAIN           1..222
FT                   /note="Kinetochore protein Spc25"
FT                   /id="PRO_0000392425"
FT   COILED          51..100
FT                   /evidence="ECO:0000255"
FT   CONFLICT        43
FT                   /note="W -> L (in Ref. 1; AAU15002)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        220
FT                   /note="C -> G (in Ref. 1; AAU15002)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   222 AA;  25678 MW;  AB44120301127BEE CRC64;
     MAIIMTESSY ERRVKALYEK QIRMEALEAK FIKKVYKFNS NLWDVKEAAC RHQRKVGKLQ
     KVIMERREEL DKRVSFIEEL DRELEATKLR SLAMKDRIKQ QKMLARQRKN EIMESIHTLS
     KTTGTYINQE ALPARVKGVT VLRGDKRNQL IPFDLKSTDV EGLDSLCQHL ESLNVDMAQW
     QQLISLAMDV AMESRAPTTP PKEAANCNSI IEIDLTSPTC HI