ID   SPC25_DROSI             Reviewed;         222 AA.
AC   B4R1Z3; Q64EW7;
DT   23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   03-AUG-2022, entry version 49.
DE   RecName: Full=Kinetochore protein Spc25 {ECO:0000250|UniProtKB:Q9V3V7};
GN   Name=Spc25 {ECO:0000250|UniProtKB:Q9V3V7};
GN   Synonyms=mitch {ECO:0000312|EMBL:AAU15000.1}; ORFNames=GD18864;
OS   Drosophila simulans (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7240;
RN   [1] {ECO:0000312|EMBL:AAU15000.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=17895365; DOI=10.1242/jcs.012112;
RA   Williams B., Leung G., Maiato H., Wong A., Li Z., Williams E.V.,
RA   Kirkpatrick C., Aquadro C.F., Rieder C.L., Goldberg M.L.;
RT   "Mitch a rapidly evolving component of the Ndc80 kinetochore complex
RT   required for correct chromosome segregation in Drosophila.";
RL   J. Cell Sci. 120:3522-3533(2007).
RN   [2] {ECO:0000312|EMBL:EDX13145.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC       Ndc80 complex, which is required for chromosome segregation and spindle
CC       checkpoint activity during meiosis and mitosis. Required for
CC       kinetochore integrity and the organization of stable microtubule
CC       binding sites in the outer plate of the kinetochore. Participates in
CC       SAC signaling that responds specifically to disruptions in spindle
CC       microtubule dynamics. The NDC80 complex synergistically enhances the
CC       affinity of the SKA1 complex for microtubules and may allow the NDC80
CC       complex to track depolymerizing microtubules.
CC       {ECO:0000250|UniProtKB:Q9HBM1, ECO:0000250|UniProtKB:Q9V3V7}.
CC   -!- SUBUNIT: Component of the Ndc80 complex, which is composed of Ndc80,
CC       Nuf2 and Spc25. {ECO:0000250|UniProtKB:Q9V3V7}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9V3V7}.
CC       Chromosome, centromere, kinetochore {ECO:0000250|UniProtKB:Q9V3V7}.
CC   -!- SIMILARITY: Belongs to the SPC25 family. {ECO:0000255}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AY714306; AAU15000.1; -; Genomic_DNA.
DR   EMBL; CM000364; EDX13145.1; -; Genomic_DNA.
DR   RefSeq; XP_002103642.1; XM_002103606.2.
DR   AlphaFoldDB; B4R1Z3; -.
DR   STRING; 7240.B4R1Z3; -.
DR   EnsemblMetazoa; FBtr0218774; FBpp0217266; FBgn0082402.
DR   GeneID; 6728293; -.
DR   HOGENOM; CLU_1246541_0_0_1; -.
DR   OMA; SNELMEC; -.
DR   PhylomeDB; B4R1Z3; -.
DR   Proteomes; UP000000304; Chromosome 3r.
DR   Bgee; FBgn0082402; Expressed in embryo and 3 other tissues.
DR   GO; GO:0031262; C:Ndc80 complex; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0051311; P:meiotic metaphase plate congression; ISS:UniProtKB.
DR   GO; GO:0000212; P:meiotic spindle organization; ISS:UniProtKB.
DR   GO; GO:0007080; P:mitotic metaphase plate congression; ISS:UniProtKB.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Centromere; Chromosome; Coiled coil;
KW   Kinetochore; Meiosis; Mitosis; Nucleus; Reference proteome.
FT   CHAIN           1..222
FT                   /note="Kinetochore protein Spc25"
FT                   /id="PRO_0000392426"
FT   COILED          51..86
FT                   /evidence="ECO:0000255"
FT   CONFLICT        124
FT                   /note="E -> G (in Ref. 1; AAU15000)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        130
FT                   /note="D -> E (in Ref. 1; AAU15000)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        157
FT                   /note="S -> A (in Ref. 1; AAU15000)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        172
FT                   /note="S -> G (in Ref. 1; AAU15000)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        177
FT                   /note="I -> M (in Ref. 1; AAU15000)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   222 AA;  25643 MW;  E603AB5FB91BB9D1 CRC64;
     MAIIMAESSY ERRVKALYEK QIRMEALEAK FIKKVYKFNS NLLDVKEAAC RHQRKVGKLQ
     KVLMERREEL DKRVSFIEEL DRELEATKLR DLAMKDRIKQ QKMLARQRKN EIMESIHTLS
     KTTETYINQD ALPARVKGVT VLRGDKRNQL IPFDLKSTDV EGLDSLCQHL ESLNVDIAQW
     QQLISLAMDV AMESRAPTTP PKEAANCNSI IEIDLTSPTC HI