ID   SPC25_DROYA             Reviewed;         223 AA.
AC   B4PPG0; Q64EW4;
DT   23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   03-AUG-2022, entry version 53.
DE   RecName: Full=Kinetochore protein Spc25 {ECO:0000250|UniProtKB:Q9V3V7};
GN   Name=Spc25 {ECO:0000250|UniProtKB:Q9V3V7};
GN   Synonyms=mitch {ECO:0000312|EMBL:AAU15003.1}; ORFNames=GE26225;
OS   Drosophila yakuba (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7245;
RN   [1] {ECO:0000312|EMBL:AAU15003.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=17895365; DOI=10.1242/jcs.012112;
RA   Williams B., Leung G., Maiato H., Wong A., Li Z., Williams E.V.,
RA   Kirkpatrick C., Aquadro C.F., Rieder C.L., Goldberg M.L.;
RT   "Mitch a rapidly evolving component of the Ndc80 kinetochore complex
RT   required for correct chromosome segregation in Drosophila.";
RL   J. Cell Sci. 120:3522-3533(2007).
RN   [2] {ECO:0000312|EMBL:EDW97166.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tai18E2 / Tucson 14021-0261.01 {ECO:0000312|EMBL:EDW97166.1};
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC       Ndc80 complex, which is required for chromosome segregation and spindle
CC       checkpoint activity during meiosis and mitosis. Required for
CC       kinetochore integrity and the organization of stable microtubule
CC       binding sites in the outer plate of the kinetochore. Participates in
CC       SAC signaling that responds specifically to disruptions in spindle
CC       microtubule dynamics. The NDC80 complex synergistically enhances the
CC       affinity of the SKA1 complex for microtubules and may allow the NDC80
CC       complex to track depolymerizing microtubules.
CC       {ECO:0000250|UniProtKB:Q9HBM1, ECO:0000250|UniProtKB:Q9V3V7}.
CC   -!- SUBUNIT: Component of the Ndc80 complex, which is composed of Ndc80,
CC       Nuf2 and Spc25. {ECO:0000250|UniProtKB:Q9V3V7}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9V3V7}.
CC       Chromosome, centromere, kinetochore {ECO:0000250|UniProtKB:Q9V3V7}.
CC   -!- SIMILARITY: Belongs to the SPC25 family. {ECO:0000255}.
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DR   EMBL; AY714309; AAU15003.1; -; Genomic_DNA.
DR   EMBL; CM000160; EDW97166.1; -; Genomic_DNA.
DR   RefSeq; XP_002097454.1; XM_002097418.2.
DR   AlphaFoldDB; B4PPG0; -.
DR   SMR; B4PPG0; -.
DR   STRING; 7245.FBpp0271235; -.
DR   EnsemblMetazoa; FBtr0272743; FBpp0271235; FBgn0082377.
DR   GeneID; 6536885; -.
DR   KEGG; dya:Dyak_GE26225; -.
DR   eggNOG; ENOG502RVT8; Eukaryota.
DR   HOGENOM; CLU_1246541_0_0_1; -.
DR   OMA; SNELMEC; -.
DR   OrthoDB; 1350883at2759; -.
DR   PhylomeDB; B4PPG0; -.
DR   Proteomes; UP000002282; Chromosome 3R.
DR   GO; GO:0031262; C:Ndc80 complex; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0051311; P:meiotic metaphase plate congression; ISS:UniProtKB.
DR   GO; GO:0000212; P:meiotic spindle organization; ISS:UniProtKB.
DR   GO; GO:0007080; P:mitotic metaphase plate congression; ISS:UniProtKB.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Centromere; Chromosome; Coiled coil;
KW   Kinetochore; Meiosis; Mitosis; Nucleus.
FT   CHAIN           1..223
FT                   /note="Kinetochore protein Spc25"
FT                   /id="PRO_0000392429"
FT   COILED          51..116
FT                   /evidence="ECO:0000255"
FT   CONFLICT        79
FT                   /note="E -> D (in Ref. 1; AAU15003)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        92..94
FT                   /note="LAM -> SVV (in Ref. 1; AAU15003)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        200
FT                   /note="P -> S (in Ref. 1; AAU15003)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   223 AA;  25629 MW;  22268E7C3116D4C0 CRC64;
     MAIIMAESSY ERRVKALYEK QIRMEALEGK FIKKVYKFNS NLLDVKEAVL RHQRKVGKLQ
     KVVMERREEL EKRVSFMEEL AQELEATKLR NLAMKDQIKQ RKMLARQRKN EIMERIHTLS
     KTTGTYVNHE ALPARVKGVT VLRGDKRDQL IPFDLNATDA EGLDSLCQHL ESLNVDVSQW
     QQLVSLAMDV AMEARAPITP PKEAANCKSI IEIDLTSPTS HQA