ID   SPC25_RAT               Reviewed;         226 AA.
AC   Q5M856;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=Kinetochore protein Spc25;
GN   Name=Spc25; Synonyms=Spbc25;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Thymus;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC       NDC80 complex, which is required for chromosome segregation and spindle
CC       checkpoint activity. Required for kinetochore integrity and the
CC       organization of stable microtubule binding sites in the outer plate of
CC       the kinetochore. The NDC80 complex synergistically enhances the
CC       affinity of the SKA1 complex for microtubules and may allow the NDC80
CC       complex to track depolymerizing microtubules.
CC       {ECO:0000250|UniProtKB:Q9HBM1}.
CC   -!- SUBUNIT: Component of the NDC80 complex, which consists of NDC80/HEC1,
CC       CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two
CC       subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each
CC       subcomplex is formed by parallel interactions through the coiled-coil
CC       domains of individual subunits. Formation of a tetrameric complex is
CC       mediated by interactions between the C-terminal regions of both
CC       subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions
CC       of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80
CC       complex has an elongated rod-like structure with globular domains at
CC       either end (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9HBM1}.
CC       Chromosome, centromere, kinetochore {ECO:0000250|UniProtKB:Q9HBM1}.
CC       Note=Localizes to kinetochores from late prophase to anaphase.
CC       Localizes specifically to the outer plate of the kinetochore.
CC       {ECO:0000250|UniProtKB:Q9HBM1}.
CC   -!- SIMILARITY: Belongs to the SPC25 family. {ECO:0000305}.
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DR   EMBL; BC088213; AAH88213.1; -; mRNA.
DR   RefSeq; NP_001009654.1; NM_001009654.1.
DR   RefSeq; XP_006234350.1; XM_006234288.3.
DR   AlphaFoldDB; Q5M856; -.
DR   SMR; Q5M856; -.
DR   STRING; 10116.ENSRNOP00000008837; -.
DR   jPOST; Q5M856; -.
DR   PaxDb; Q5M856; -.
DR   PRIDE; Q5M856; -.
DR   Ensembl; ENSRNOT00000008837; ENSRNOP00000008837; ENSRNOG00000006731.
DR   GeneID; 295661; -.
DR   KEGG; rno:295661; -.
DR   UCSC; RGD:1307811; rat.
DR   CTD; 57405; -.
DR   RGD; 1307811; Spc25.
DR   eggNOG; KOG4657; Eukaryota.
DR   GeneTree; ENSGT00390000002220; -.
DR   HOGENOM; CLU_102420_0_0_1; -.
DR   InParanoid; Q5M856; -.
DR   OMA; QGDYEVT; -.
DR   OrthoDB; 1460234at2759; -.
DR   PhylomeDB; Q5M856; -.
DR   TreeFam; TF332941; -.
DR   Reactome; R-RNO-141444; Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
DR   Reactome; R-RNO-2467813; Separation of Sister Chromatids.
DR   Reactome; R-RNO-2500257; Resolution of Sister Chromatid Cohesion.
DR   Reactome; R-RNO-5663220; RHO GTPases Activate Formins.
DR   Reactome; R-RNO-68877; Mitotic Prometaphase.
DR   Reactome; R-RNO-9648025; EML4 and NUDC in mitotic spindle formation.
DR   PRO; PR:Q5M856; -.
DR   Proteomes; UP000002494; Chromosome 3.
DR   Bgee; ENSRNOG00000006731; Expressed in thymus and 20 other tissues.
DR   Genevisible; Q5M856; RN.
DR   GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR   GO; GO:0000776; C:kinetochore; ISS:UniProtKB.
DR   GO; GO:0031262; C:Ndc80 complex; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0008608; P:attachment of spindle microtubules to kinetochore; ISO:RGD.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0007059; P:chromosome segregation; ISS:UniProtKB.
DR   GO; GO:0007052; P:mitotic spindle organization; ISS:UniProtKB.
DR   InterPro; IPR045143; Spc25.
DR   InterPro; IPR013255; Spc25_C.
DR   PANTHER; PTHR14281; PTHR14281; 1.
DR   Pfam; PF08234; Spindle_Spc25; 1.
PE   2: Evidence at transcript level;
KW   Cell cycle; Cell division; Centromere; Chromosome; Coiled coil;
KW   Kinetochore; Mitosis; Nucleus; Phosphoprotein; Reference proteome.
FT   CHAIN           1..226
FT                   /note="Kinetochore protein Spc25"
FT                   /id="PRO_0000249567"
FT   REGION          1..146
FT                   /note="Interaction with the N-terminus of SPBC24"
FT                   /evidence="ECO:0000250"
FT   REGION          1..58
FT                   /note="Interaction with the NDC80-NUF2 subcomplex"
FT                   /evidence="ECO:0000250"
FT   REGION          147..226
FT                   /note="Interaction with the C-terminus of SPBC24"
FT                   /evidence="ECO:0000250"
FT   COILED          89..147
FT                   /evidence="ECO:0000255"
FT   MOD_RES         12
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9HBM1"
SQ   SEQUENCE   226 AA;  26189 MW;  DB87BD63C8390A2A CRC64;
     MGEDELAAFE KSINEFGDKF RYRLSDNRSQ VLGLKDAFKD SIRALSEKLS LKLKEEERMA
     EMILEYKNQL CKQNKLIQEK KDNVLKMIAE VKGKGQEVEV LTANIQDLKE EYARKKETIS
     TANKANEERL KRLQKSADLY RDRLGLEIRK IPGDKLQFIF TSIDPKNPES PFMFSLSINE
     AKEYEVSDCS PHLECLAEFQ EKVRKTNNFS AFLANVRKAF IAKVHN