SPC25_SCHPO
ID SPC25_SCHPO Reviewed; 238 AA.
AC Q10430;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Kinetochore protein spc25;
GN Name=spc25; ORFNames=SPCC188.04c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP IDENTIFICATION IN THE NDC80 COMPLEX, AND IDENTIFICATION IN THE NMS COMPLEX.
RX PubMed=16079914; DOI=10.1038/sj.emboj.7600762;
RA Liu X., McLeod I., Anderson S., Yates J.R. III, He X.;
RT "Molecular analysis of kinetochore architecture in fission yeast.";
RL EMBO J. 24:2919-2930(2005).
RN [3]
RP FUNCTION OF THE NDC80 COMPLEX, INTERACTION WITH NDC80 AND SPC24, AND
RP SUBCELLULAR LOCATION.
RX PubMed=15728720; DOI=10.1091/mbc.e04-11-0996;
RA Asakawa H., Hayashi A., Haraguchi T., Hiraoka Y.;
RT "Dissociation of the Nuf2-Ndc80 complex releases centromeres from the
RT spindle-pole body during meiotic prophase in fission yeast.";
RL Mol. Biol. Cell 16:2325-2338(2005).
RN [4]
RP IDENTIFICATION IN THE NMS COMPLEX.
RX PubMed=17035632; DOI=10.1091/mbc.e06-05-0388;
RA Hayashi A., Asakawa H., Haraguchi T., Hiraoka Y.;
RT "Reconstruction of the kinetochore during meiosis in fission yeast
RT Schizosaccharomyces pombe.";
RL Mol. Biol. Cell 17:5173-5184(2006).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Acts as a component of the NMS (Ndc80-MIND-Spc7) super
CC complex which has a role in kinetochore function during late meiotic
CC prophase and throughout the mitotic cell cycle. Acts as a component of
CC the essential kinetochore-associated NDC80 complex, which is required
CC for chromosome segregation and spindle checkpoint activity.
CC {ECO:0000269|PubMed:15728720}.
CC -!- SUBUNIT: Component of the NDC80 complex, which consists of ndc80, nuf2,
CC spc24 and spc25. Can self-associate. Component of the NMS super complex
CC which consists of mis12, mis13, mis14, ndc80, nnf1, nuf2, sos7, spc7,
CC spc24 and spc25. {ECO:0000269|PubMed:16079914,
CC ECO:0000269|PubMed:17035632}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15728720}.
CC Chromosome, centromere, kinetochore {ECO:0000269|PubMed:15728720,
CC ECO:0000269|PubMed:16823372}. Note=Associated with kinetochores.
CC {ECO:0000250|UniProtKB:P40014}.
CC -!- SIMILARITY: Belongs to the SPC25 family. {ECO:0000305}.
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DR EMBL; CU329672; CAB41224.1; -; Genomic_DNA.
DR PIR; T41184; T41184.
DR RefSeq; NP_588208.1; NM_001023198.2.
DR AlphaFoldDB; Q10430; -.
DR SMR; Q10430; -.
DR BioGRID; 275771; 5.
DR ComplexPortal; CPX-549; Ndc80 complex.
DR IntAct; Q10430; 2.
DR STRING; 4896.SPCC188.04c.1; -.
DR MaxQB; Q10430; -.
DR PaxDb; Q10430; -.
DR PRIDE; Q10430; -.
DR EnsemblFungi; SPCC188.04c.1; SPCC188.04c.1:pep; SPCC188.04c.
DR GeneID; 2539200; -.
DR KEGG; spo:SPCC188.04c; -.
DR PomBase; SPCC188.04c; spc25.
DR VEuPathDB; FungiDB:SPCC188.04c; -.
DR eggNOG; KOG4657; Eukaryota.
DR HOGENOM; CLU_065188_0_0_1; -.
DR InParanoid; Q10430; -.
DR OMA; HEDQRMK; -.
DR PhylomeDB; Q10430; -.
DR PRO; PR:Q10430; -.
DR Proteomes; UP000002485; Chromosome III.
DR GO; GO:0000776; C:kinetochore; IDA:PomBase.
DR GO; GO:0031262; C:Ndc80 complex; IDA:PomBase.
DR GO; GO:0031617; C:NMS complex; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; IC:PomBase.
DR GO; GO:0008608; P:attachment of spindle microtubules to kinetochore; IC:ComplexPortal.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IC:ComplexPortal.
DR GO; GO:0007094; P:mitotic spindle assembly checkpoint signaling; IC:ComplexPortal.
DR GO; GO:0051455; P:monopolar spindle attachment to meiosis I kinetochore; IC:PomBase.
DR InterPro; IPR045143; Spc25.
DR InterPro; IPR013255; Spc25_C.
DR PANTHER; PTHR14281; PTHR14281; 1.
DR Pfam; PF08234; Spindle_Spc25; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Coiled coil;
KW Kinetochore; Meiosis; Mitosis; Nucleus; Reference proteome.
FT CHAIN 1..238
FT /note="Kinetochore protein spc25"
FT /id="PRO_0000116881"
FT COILED 28..141
FT /evidence="ECO:0000255"
SQ SEQUENCE 238 AA; 28811 MW; 7351F2E8CDFC2659 CRC64;
MSLANFPTIE LDYDSLKSKI SNFNSIFDRF LQEERKKLLN NKNEYLRQLS EINEAQKKAE
KSLEQTEARK QNFTELLEKE HEEQAITEQE IFSFQEKLDA MLKRKQKLSE ELDHYRAIIS
SKRELRAQEM EAKRKQDSYN NPELKFWEDY LGLKMEGVHD EVIRFIFTNI DEKDWNKQFS
FQINLAERDY KVVHCHPPLP HVDDLVNKVN RTRDFYQFLK DMRKGFRELH RKDLSQLI
