ID   SPC25_XENLA             Reviewed;         228 AA.
AC   Q6SKR5; Q6DDZ6;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   19-SEP-2006, sequence version 2.
DT   03-AUG-2022, entry version 59.
DE   RecName: Full=Kinetochore protein Spc25;
DE            Short=xSpc25;
GN   Name=spc25; Synonyms=spbc25;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY,
RP   IDENTIFICATION IN THE NDC80 COMPLEX, AND SUBCELLULAR LOCATION.
RX   PubMed=14738735; DOI=10.1016/j.cub.2003.12.058;
RA   McCleland M.L., Kallio M.J., Barrett-Wilt G.A., Kestner C.A.,
RA   Shabanowitz J., Hunt D.F., Gorbsky G.J., Stukenberg P.T.;
RT   "The vertebrate Ndc80 complex contains Spc24 and Spc25 homologs, which are
RT   required to establish and maintain kinetochore-microtubule attachment.";
RL   Curr. Biol. 14:131-137(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   CHARACTERIZATION OF THE NDC80 COMPLEX, AND INTERACTION OF THE NDC80 COMPLEX
RP   WITH MIS12 AND ZWINT.
RX   PubMed=16079178; DOI=10.1091/mbc.e05-03-0239;
RA   Emanuele M.J., McCleland M.L., Satinover D.L., Stukenberg P.T.;
RT   "Measuring the stoichiometry and physical interactions between components
RT   elucidates the architecture of the vertebrate kinetochore.";
RL   Mol. Biol. Cell 16:4882-4892(2005).
CC   -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC       NDC80 complex, which is required for chromosome segregation and spindle
CC       checkpoint activity (PubMed:14738735). Required for kinetochore
CC       integrity and the organization of stable microtubule binding sites in
CC       the outer plate of the kinetochore (PubMed:14738735). The NDC80 complex
CC       synergistically enhances the affinity of the SKA1 complex for
CC       microtubules and may allow the NDC80 complex to track depolymerizing
CC       microtubules. {ECO:0000250|UniProtKB:Q9HBM1,
CC       ECO:0000269|PubMed:14738735}.
CC   -!- SUBUNIT: Component of the NDC80 complex, which is composed of ndc80,
CC       cdca1, spbc24 and spbc25. The NDC80 complex interacts with mis12 and
CC       zwint. {ECO:0000269|PubMed:14738735, ECO:0000269|PubMed:16079178}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14738735}.
CC       Chromosome, centromere, kinetochore {ECO:0000269|PubMed:14738735}.
CC       Note=Localizes to kinetochores from late prophase to anaphase.
CC       {ECO:0000250|UniProtKB:Q9HBM1}.
CC   -!- SIMILARITY: Belongs to the SPC25 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAH77354.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AY456386; AAR88650.1; -; mRNA.
DR   EMBL; BC077354; AAH77354.1; ALT_INIT; mRNA.
DR   RefSeq; NP_001093334.2; NM_001099864.1.
DR   AlphaFoldDB; Q6SKR5; -.
DR   SMR; Q6SKR5; -.
DR   IntAct; Q6SKR5; 3.
DR   MaxQB; Q6SKR5; -.
DR   GeneID; 100101271; -.
DR   KEGG; xla:100101271; -.
DR   CTD; 100101271; -.
DR   Xenbase; XB-GENE-950634; spc25.L.
DR   OMA; QGDYEVT; -.
DR   OrthoDB; 1460234at2759; -.
DR   Proteomes; UP000186698; Chromosome 8L.
DR   Bgee; 100101271; Expressed in egg cell and 17 other tissues.
DR   GO; GO:0000776; C:kinetochore; ISS:UniProtKB.
DR   GO; GO:0031262; C:Ndc80 complex; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0007059; P:chromosome segregation; ISS:UniProtKB.
DR   GO; GO:0007052; P:mitotic spindle organization; ISS:UniProtKB.
DR   InterPro; IPR045143; Spc25.
DR   InterPro; IPR013255; Spc25_C.
DR   PANTHER; PTHR14281; PTHR14281; 1.
DR   Pfam; PF08234; Spindle_Spc25; 1.
PE   1: Evidence at protein level;
KW   Cell cycle; Cell division; Centromere; Chromosome; Coiled coil;
KW   Kinetochore; Mitosis; Nucleus; Reference proteome.
FT   CHAIN           1..228
FT                   /note="Kinetochore protein Spc25"
FT                   /id="PRO_0000249568"
FT   COILED          61..144
FT                   /evidence="ECO:0000255"
FT   CONFLICT        226
FT                   /note="Q -> K (in Ref. 1; AAR88650)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   228 AA;  26704 MW;  8B4916508876C344 CRC64;
     MSALKMDDEQ SLNVYMQEFR TRFINKSSDE MTSQALKESY KESAATDAWF KKYRDGELMI
     EKVLEFRNEI TLQNKRIEEK QKNILQEAAK QDDHEQLKGE LTERIQRLRE ELNQKREVAL
     ANRKANKERI KELQKSATLF KERLGLEIRK LRGDKLQFVF RCINPKDLDQ PYSCIISLNA
     EGDYEVAGCD PPLECIAEFQ EKIRETRNFS ALLANLRKSF TALGSQVK