ID   SPC25_XENTR             Reviewed;         228 AA.
AC   Q6DF39;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Kinetochore protein Spc25;
GN   Name=spc25; Synonyms=spbc25; ORFNames=TEgg030h02.1;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Egg;
RG   Sanger Xenopus tropicalis EST/cDNA project;
RL   Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=F6;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC       NDC80 complex, which is required for chromosome segregation and spindle
CC       checkpoint activity. Required for kinetochore integrity and the
CC       organization of stable microtubule binding sites in the outer plate of
CC       the kinetochore. The NDC80 complex synergistically enhances the
CC       affinity of the SKA1 complex for microtubules and may allow the NDC80
CC       complex to track depolymerizing microtubules.
CC       {ECO:0000250|UniProtKB:Q9HBM1}.
CC   -!- SUBUNIT: Component of the NDC80 complex, which is composed of ndc80,
CC       cdca1, spbc24 and spbc25. The NDC80 complex interacts with mis12 and
CC       zwint (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9HBM1}.
CC       Chromosome, centromere, kinetochore {ECO:0000250|UniProtKB:Q9HBM1}.
CC       Note=Localizes to kinetochores from late prophase to anaphase.
CC       Localizes specifically to the outer plate of the kinetochore.
CC       {ECO:0000250|UniProtKB:Q9HBM1}.
CC   -!- SIMILARITY: Belongs to the SPC25 family. {ECO:0000305}.
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DR   EMBL; CR761196; CAJ81876.1; -; mRNA.
DR   EMBL; BC076904; AAH76904.1; -; mRNA.
DR   RefSeq; NP_001005041.1; NM_001005041.1.
DR   RefSeq; XP_012823697.1; XM_012968243.2.
DR   RefSeq; XP_012823698.1; XM_012968244.2.
DR   RefSeq; XP_012823699.1; XM_012968245.2.
DR   RefSeq; XP_012823700.1; XM_012968246.2.
DR   AlphaFoldDB; Q6DF39; -.
DR   SMR; Q6DF39; -.
DR   STRING; 8364.ENSXETP00000022695; -.
DR   PaxDb; Q6DF39; -.
DR   DNASU; 448571; -.
DR   GeneID; 448571; -.
DR   KEGG; xtr:448571; -.
DR   CTD; 57405; -.
DR   Xenbase; XB-GENE-950628; spc25.
DR   eggNOG; KOG4657; Eukaryota.
DR   HOGENOM; CLU_102420_0_0_1; -.
DR   InParanoid; Q6DF39; -.
DR   OMA; HEYTDEA; -.
DR   OrthoDB; 1460234at2759; -.
DR   TreeFam; TF332941; -.
DR   Reactome; R-XTR-141444; Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
DR   Reactome; R-XTR-2467813; Separation of Sister Chromatids.
DR   Reactome; R-XTR-2500257; Resolution of Sister Chromatid Cohesion.
DR   Reactome; R-XTR-5663220; RHO GTPases Activate Formins.
DR   Reactome; R-XTR-9648025; EML4 and NUDC in mitotic spindle formation.
DR   Proteomes; UP000008143; Chromosome 8.
DR   Proteomes; UP000790000; Unplaced.
DR   Bgee; ENSXETG00000010306; Expressed in ovary and 12 other tissues.
DR   ExpressionAtlas; Q6DF39; baseline.
DR   GO; GO:0000776; C:kinetochore; ISS:UniProtKB.
DR   GO; GO:0031262; C:Ndc80 complex; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0007059; P:chromosome segregation; ISS:UniProtKB.
DR   GO; GO:0007052; P:mitotic spindle organization; ISS:UniProtKB.
DR   InterPro; IPR045143; Spc25.
DR   InterPro; IPR013255; Spc25_C.
DR   PANTHER; PTHR14281; PTHR14281; 1.
DR   Pfam; PF08234; Spindle_Spc25; 1.
PE   2: Evidence at transcript level;
KW   Cell cycle; Cell division; Centromere; Chromosome; Coiled coil;
KW   Kinetochore; Mitosis; Nucleus; Reference proteome.
FT   CHAIN           1..228
FT                   /note="Kinetochore protein Spc25"
FT                   /id="PRO_0000249569"
FT   COILED          61..144
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   228 AA;  26858 MW;  5923C453F44CCC6D CRC64;
     MSALKMDEEQ SLNVYMQEFR TRFINRSSEE VTSQALKESY KDSAQTDAWF KKYRDGELMI
     EKVLEFRNEI TLQNKRIEEK QKNILQEAAK QGEHEQIKEE LTERIQRLRE ELNKKREVAL
     ANRKANKERI KELQKSATLF RERLGLEIRK LRGDKLQFVF RCINPKDLDQ PYSCIISLNA
     EGEYEVTGCD PPLECIAEFQ EKVRETRNFS ALLANLRKSF TALGSQVK