SPC25_YEAST
ID SPC25_YEAST Reviewed; 221 AA.
AC P40014; D3DLR6;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 165.
DE RecName: Full=Kinetochore protein SPC25;
GN Name=SPC25; OrderedLocusNames=YER018C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169868;
RA Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA Botstein D., Davis R.W.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL Nature 387:78-81(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 67-221.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7754704; DOI=10.1002/yea.320100903;
RA Schnall R., Mannhaupt G., Stucka R., Tauer R., Ehnle S., Schwarzlose C.,
RA Vetter I., Feldmann H.;
RT "Identification of a set of yeast genes coding for a novel family of
RT putative ATPases with high similarity to constituents of the 26S protease
RT complex.";
RL Yeast 10:1141-1155(1994).
RN [4]
RP SUBCELLULAR LOCATION, AND INTERACTION WITH TID3 AND NUF2.
RX PubMed=11179222; DOI=10.1093/emboj/20.4.777;
RA Janke C., Ortiz J., Lechner J., Shevchenko A., Shevchenko A., Magiera M.M.,
RA Schramm C., Schiebel E.;
RT "The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and
RT Nuf2p at the kinetochore and are important for kinetochore clustering and
RT checkpoint control.";
RL EMBO J. 20:777-791(2001).
RN [5]
RP INTERACTION WITH NDC80 AND SPC24.
RX PubMed=11952896; DOI=10.1046/j.1365-2958.2002.02844.x;
RA Le Masson I., Saveanu C., Chevalier A., Namane A., Gobin R.,
RA Fromont-Racine M., Jacquier A., Mann C.;
RT "Spc24 interacts with Mps2 and is required for chromosome segregation, but
RT is not implicated in spindle pole body duplication.";
RL Mol. Microbiol. 43:1431-1443(2002).
RN [6]
RP FUNCTION OF THE NDC80 COMPLEX, SUBCELLULAR LOCATION, AND IDENTIFICATION IN
RP THE NDC80 COMPLEX.
RX PubMed=11266451; DOI=10.1083/jcb.152.2.349;
RA Wigge P.A., Kilmartin J.V.;
RT "The Ndc80p complex from Saccharomyces cerevisiae contains conserved
RT centromere components and has a function in chromosome segregation.";
RL J. Cell Biol. 152:349-360(2001).
RN [7]
RP FUNCTION OF THE NDC80 COMPLEX.
RX PubMed=12514103; DOI=10.1101/gad.1040903;
RA McCleland M.L., Gardner R.D., Kallio M.J., Daum J.R., Gorbsky G.J.,
RA Burke D.J., Stukenberg P.T.;
RT "The highly conserved Ndc80 complex is required for kinetochore assembly,
RT chromosome congression, and spindle checkpoint activity.";
RL Genes Dev. 17:101-114(2003).
RN [8]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [9]
RP IDENTIFICATION IN THE NDC80 COMPLEX.
RX PubMed=15371542; DOI=10.1091/mbc.e04-06-0443;
RA Kerres A., Vietmeier-Decker C., Ortiz J., Karig I., Beuter C., Hegemann J.,
RA Lechner J., Fleig U.;
RT "The fission yeast kinetochore component Spc7 associates with the EB1
RT family member Mal3 and is required for kinetochore-spindle association.";
RL Mol. Biol. Cell 15:5255-5267(2004).
RN [10]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [11]
RP 3D-STRUCTURE MODELING OF THE NDC80 COMPLEX.
RX PubMed=15809444; DOI=10.1073/pnas.0501168102;
RA Wei R.R., Sorger P.K., Harrison S.C.;
RT "Molecular organization of the Ndc80 complex, an essential kinetochore
RT component.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:5363-5367(2005).
CC -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC NDC80 complex, which is involved in chromosome segregation and spindle
CC checkpoint activity. {ECO:0000269|PubMed:11266451,
CC ECO:0000269|PubMed:12514103}.
CC -!- SUBUNIT: Component of the NDC80 complex, which consists of TID3/NDC80,
CC NUF2, SPC24 and SPC25. The NDC80 complex is formed by two subcomplexes,
CC TID3/NDC80-NUF2 and SPC24-SPC25, which are joined end-to-end through
CC their coiled-coil domains. It has a rod-like structure with a length of
CC 570 Angstroms and globular domains at either end. The TID3/NDC80-NUF2
CC globular domains are probably directed to microtubules, the SPC24-SPC25
CC globular domains to the centromere. {ECO:0000269|PubMed:11266451,
CC ECO:0000269|PubMed:15371542}.
CC -!- INTERACTION:
CC P40014; P40460: NDC80; NbExp=13; IntAct=EBI-22458, EBI-25247;
CC P40014; P33895: NUF2; NbExp=6; IntAct=EBI-22458, EBI-12377;
CC P40014; Q04477: SPC24; NbExp=23; IntAct=EBI-22458, EBI-27228;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11179222}.
CC Chromosome, centromere, kinetochore {ECO:0000269|PubMed:11179222,
CC ECO:0000269|PubMed:11266451}. Note=Associated with kinetochores.
CC {ECO:0000269|PubMed:11179222}.
CC -!- MISCELLANEOUS: Present with 3280 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the SPC25 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U18778; AAB64551.1; -; Genomic_DNA.
DR EMBL; X81066; CAA56952.1; -; Genomic_DNA.
DR EMBL; BK006939; DAA07670.1; -; Genomic_DNA.
DR PIR; S50476; S50476.
DR RefSeq; NP_010934.3; NM_001178909.3.
DR PDB; 2FTX; X-ray; 1.90 A; A=133-221.
DR PDB; 2FV4; NMR; -; A=128-221.
DR PDB; 4GEQ; X-ray; 2.01 A; A/C=133-221.
DR PDB; 5T6J; X-ray; 1.75 A; B=133-221.
DR PDB; 5TCS; X-ray; 2.83 A; D=1-31, D=138-221.
DR PDB; 5TD8; X-ray; 7.53 A; D=1-221.
DR PDB; 7KDF; X-ray; 2.72 A; D=137-221.
DR PDBsum; 2FTX; -.
DR PDBsum; 2FV4; -.
DR PDBsum; 4GEQ; -.
DR PDBsum; 5T6J; -.
DR PDBsum; 5TCS; -.
DR PDBsum; 5TD8; -.
DR PDBsum; 7KDF; -.
DR AlphaFoldDB; P40014; -.
DR SMR; P40014; -.
DR BioGRID; 36751; 182.
DR ComplexPortal; CPX-548; NDC80 complex.
DR DIP; DIP-2990N; -.
DR IntAct; P40014; 13.
DR MINT; P40014; -.
DR STRING; 4932.YER018C; -.
DR iPTMnet; P40014; -.
DR MaxQB; P40014; -.
DR PaxDb; P40014; -.
DR PRIDE; P40014; -.
DR EnsemblFungi; YER018C_mRNA; YER018C; YER018C.
DR GeneID; 856738; -.
DR KEGG; sce:YER018C; -.
DR SGD; S000000820; SPC25.
DR VEuPathDB; FungiDB:YER018C; -.
DR eggNOG; KOG4657; Eukaryota.
DR HOGENOM; CLU_085127_1_0_1; -.
DR InParanoid; P40014; -.
DR OMA; TTRAQWR; -.
DR BioCyc; YEAST:G3O-30203-MON; -.
DR EvolutionaryTrace; P40014; -.
DR PRO; PR:P40014; -.
DR Proteomes; UP000002311; Chromosome V.
DR RNAct; P40014; protein.
DR GO; GO:0000776; C:kinetochore; IDA:SGD.
DR GO; GO:0031262; C:Ndc80 complex; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0098653; P:centromere clustering; IMP:SGD.
DR GO; GO:0007059; P:chromosome segregation; IMP:SGD.
DR InterPro; IPR038066; Spc24_Fungi_globular_sf.
DR SUPFAM; SSF143026; SSF143026; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Cell cycle; Cell division; Centromere;
KW Chromosome; Coiled coil; Kinetochore; Mitosis; Nucleus; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22814378"
FT CHAIN 2..221
FT /note="Kinetochore protein SPC25"
FT /id="PRO_0000202622"
FT REGION 76..101
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 9..112
FT /evidence="ECO:0000255"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT HELIX 133..145
FT /evidence="ECO:0007829|PDB:5T6J"
FT STRAND 147..151
FT /evidence="ECO:0007829|PDB:5T6J"
FT STRAND 153..155
FT /evidence="ECO:0007829|PDB:5T6J"
FT STRAND 158..164
FT /evidence="ECO:0007829|PDB:5T6J"
FT STRAND 169..173
FT /evidence="ECO:0007829|PDB:5T6J"
FT STRAND 175..178
FT /evidence="ECO:0007829|PDB:5T6J"
FT STRAND 180..186
FT /evidence="ECO:0007829|PDB:5T6J"
FT HELIX 190..201
FT /evidence="ECO:0007829|PDB:5T6J"
FT HELIX 206..220
FT /evidence="ECO:0007829|PDB:5T6J"
SQ SEQUENCE 221 AA; 25244 MW; 1BED6CCFF330A5D8 CRC64;
MASIDAFSDL ERRMDGFQKD VAQVLARQQN HARQQLQQFQ AEMRQLHNQH QHLIDELQRL
ATQRTALQQQ IHAAQQATNT TREQWRSYHE RESELSRRQS TLAAQSRELD SLLQQRGKEC
VQLRARWAAQ SGNDAAEVAL YERLLQLRVL PGASDVHDVR FVFGDDSRCW IEVAMHGDHV
IGNSHPALDP KSRATLEHVL TVQGDLAAFL VVARDMLLAS L
