SPC34_ASHGO
ID SPC34_ASHGO Reviewed; 290 AA.
AC Q758R8;
DT 21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 85.
DE RecName: Full=DASH complex subunit SPC34;
DE AltName: Full=Outer kinetochore protein SPC34;
GN Name=SPC34; OrderedLocusNames=AEL315W;
OS Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS (Yeast) (Eremothecium gossypii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX NCBI_TaxID=284811;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX PubMed=15001715; DOI=10.1126/science.1095781;
RA Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA Gaffney T.D., Philippsen P.;
RT "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT cerevisiae genome.";
RL Science 304:304-307(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX PubMed=23749448; DOI=10.1534/g3.112.002881;
RA Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT loci, numerous translocations, lack of transposons, and distinct gene
RT duplications.";
RL G3 (Bethesda) 3:1225-1239(2013).
CC -!- FUNCTION: Component of the DASH complex, a microtubule-binding
CC subcomplex of the outer kinetochore that is essential for proper
CC chromosome segregation. The DASH complex mediates the formation and
CC maintenance of bipolar kinetochore-microtubule attachments by forming
CC closed rings around spindle microtubules and establishing interactions
CC with proteins from the central kinetochore (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: The DASH complex oligomerizes to form rings that encircle the
CC microtubules. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm, cytoskeleton,
CC spindle {ECO:0000250}. Chromosome, centromere, kinetochore
CC {ECO:0000250}. Note=Associates with the mitotic spindle and the
CC kinetochore. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DASH complex SPC34 family. {ECO:0000305}.
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DR EMBL; AE016818; AAS52369.1; -; Genomic_DNA.
DR RefSeq; NP_984545.1; NM_209898.1.
DR AlphaFoldDB; Q758R8; -.
DR SMR; Q758R8; -.
DR STRING; 33169.AAS52369; -.
DR EnsemblFungi; AAS52369; AAS52369; AGOS_AEL315W.
DR GeneID; 4620717; -.
DR KEGG; ago:AGOS_AEL315W; -.
DR eggNOG; ENOG502QSS0; Eukaryota.
DR HOGENOM; CLU_970457_0_0_1; -.
DR InParanoid; Q758R8; -.
DR OMA; LIRDCNP; -.
DR Proteomes; UP000000591; Chromosome V.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0042729; C:DASH complex; IEA:EnsemblFungi.
DR GO; GO:0005876; C:spindle microtubule; IEA:InterPro.
DR GO; GO:0051010; F:microtubule plus-end binding; IEA:EnsemblFungi.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:1990758; P:mitotic sister chromatid biorientation; IEA:EnsemblFungi.
DR GO; GO:0051987; P:positive regulation of attachment of spindle microtubules to kinetochore; IEA:EnsemblFungi.
DR GO; GO:0031116; P:positive regulation of microtubule polymerization; IEA:EnsemblFungi.
DR InterPro; IPR013966; Spc34.
DR Pfam; PF08657; DASH_Spc34; 1.
PE 3: Inferred from homology;
KW Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition;
KW Coiled coil; Cytoplasm; Cytoskeleton; Kinetochore; Microtubule; Mitosis;
KW Nucleus; Reference proteome.
FT CHAIN 1..290
FT /note="DASH complex subunit SPC34"
FT /id="PRO_0000211550"
FT REGION 238..262
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 220..290
FT /evidence="ECO:0000255"
SQ SEQUENCE 290 AA; 33394 MW; 09533353E3E0706D CRC64;
MSESLSYCLD QIKESADSIA TLYFKPPGIF QNALVHNSKT SYAEIITRLI RDADPVDEMS
LYMTDKGGKL RRKDGKQGVY DHLTERAATL KRARLLGAPE ETPIVHVPKE FYLKQHDIIT
KKKQRQNRDF LFDEFSKESG GMFEVLIKKF AGDQQVRNLL LALQNGSVIT GEDASSRRKT
MFVEDFSVEL ILRLLREIVT QWPLNEHRTK YEHLLQTYHD ISDATNKLRA QIADQEHRLQ
SQDALSEEPD ADDDDVAHPT SGVSYLIQQE LDEIDALERE LEQLRRDSRT