ID   SPC34_CANGA             Reviewed;         307 AA.
AC   Q6FLI2;
DT   21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=DASH complex subunit SPC34;
DE   AltName: Full=Outer kinetochore protein SPC34;
GN   Name=SPC34; OrderedLocusNames=CAGL0L03223g;
OS   Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL
OS   Y-65) (Yeast) (Torulopsis glabrata).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Nakaseomyces;
OC   Nakaseomyces/Candida clade.
OX   NCBI_TaxID=284593;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Component of the DASH complex, a microtubule-binding
CC       subcomplex of the outer kinetochore that is essential for proper
CC       chromosome segregation. The DASH complex mediates the formation and
CC       maintenance of bipolar kinetochore-microtubule attachments by forming
CC       closed rings around spindle microtubules and establishing interactions
CC       with proteins from the central kinetochore (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: The DASH complex oligomerizes to form rings that encircle the
CC       microtubules. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm, cytoskeleton,
CC       spindle {ECO:0000250}. Chromosome, centromere, kinetochore
CC       {ECO:0000250}. Note=Associates with the mitotic spindle and the
CC       kinetochore. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DASH complex SPC34 family. {ECO:0000305}.
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DR   EMBL; CR380958; CAG61882.1; -; Genomic_DNA.
DR   RefSeq; XP_448912.1; XM_448912.1.
DR   AlphaFoldDB; Q6FLI2; -.
DR   SMR; Q6FLI2; -.
DR   STRING; 5478.XP_448912.1; -.
DR   EnsemblFungi; CAG61882; CAG61882; CAGL0L03223g.
DR   GeneID; 2890851; -.
DR   KEGG; cgr:CAGL0L03223g; -.
DR   CGD; CAL0135020; CAGL0L03223g.
DR   VEuPathDB; FungiDB:CAGL0L03223g; -.
DR   eggNOG; ENOG502QSS0; Eukaryota.
DR   HOGENOM; CLU_970457_0_0_1; -.
DR   InParanoid; Q6FLI2; -.
DR   OMA; LIRDCNP; -.
DR   Proteomes; UP000002428; Chromosome L.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0042729; C:DASH complex; IEA:EnsemblFungi.
DR   GO; GO:0005876; C:spindle microtubule; IEA:InterPro.
DR   GO; GO:0051010; F:microtubule plus-end binding; IEA:EnsemblFungi.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:1990758; P:mitotic sister chromatid biorientation; IEA:EnsemblFungi.
DR   GO; GO:0051987; P:positive regulation of attachment of spindle microtubules to kinetochore; IEA:EnsemblFungi.
DR   GO; GO:0031116; P:positive regulation of microtubule polymerization; IEA:EnsemblFungi.
DR   InterPro; IPR013966; Spc34.
DR   Pfam; PF08657; DASH_Spc34; 1.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition;
KW   Coiled coil; Cytoplasm; Cytoskeleton; Kinetochore; Microtubule; Mitosis;
KW   Nucleus; Reference proteome.
FT   CHAIN           1..307
FT                   /note="DASH complex subunit SPC34"
FT                   /id="PRO_0000211551"
FT   COILED          234..307
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   307 AA;  35524 MW;  08388E7BED523222 CRC64;
     MGESMNSCLE EINGCLDSIT SLYFKPPGIF HNAMIRISKD GKETHTLNDK IKNLIKGCDP
     KEETTLFQLD PVTKSLRRKD GRQALFDYLD KRDLNMKRNR RLGHKDEKPV IHVPNEFYIR
     EHDEELLRDS QRTKRFKAAG HGNDIDSNNT NNTTTALITE LFNDDVGLMA TMRKKFKEND
     EIKNLLMALQ KGSVILDEKE GISTNNRRRT LFVEDFSTES ILKLLDAVLE MFPTEEYKKA
     AEQYHDVFQE LNEESEKLLA EIQLQKDEIE GRLKIESSKD STTGVQKLIE KERQKIAELE
     SQLRISN