ID   SPC34_KLULA             Reviewed;         275 AA.
AC   Q6CJ49;
DT   21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=DASH complex subunit SPC34;
DE   AltName: Full=Outer kinetochore protein SPC34;
GN   Name=SPC34; OrderedLocusNames=KLLA0F21428g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Component of the DASH complex, a microtubule-binding
CC       subcomplex of the outer kinetochore that is essential for proper
CC       chromosome segregation. The DASH complex mediates the formation and
CC       maintenance of bipolar kinetochore-microtubule attachments by forming
CC       closed rings around spindle microtubules and establishing interactions
CC       with proteins from the central kinetochore (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: The DASH complex oligomerizes to form rings that encircle the
CC       microtubules. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm, cytoskeleton,
CC       spindle {ECO:0000250}. Chromosome, centromere, kinetochore
CC       {ECO:0000250}. Note=Associates with the mitotic spindle and the
CC       kinetochore. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DASH complex SPC34 family. {ECO:0000305}.
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DR   EMBL; CR382126; CAG98748.1; -; Genomic_DNA.
DR   RefSeq; XP_456040.1; XM_456040.1.
DR   AlphaFoldDB; Q6CJ49; -.
DR   SMR; Q6CJ49; -.
DR   STRING; 28985.XP_456040.1; -.
DR   EnsemblFungi; CAG98748; CAG98748; KLLA0_F21428g.
DR   GeneID; 2895447; -.
DR   KEGG; kla:KLLA0_F21428g; -.
DR   eggNOG; ENOG502QSS0; Eukaryota.
DR   HOGENOM; CLU_970457_0_0_1; -.
DR   InParanoid; Q6CJ49; -.
DR   OMA; LIRDCNP; -.
DR   Proteomes; UP000000598; Chromosome F.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0042729; C:DASH complex; IEA:EnsemblFungi.
DR   GO; GO:0005876; C:spindle microtubule; IEA:InterPro.
DR   GO; GO:0051010; F:microtubule plus-end binding; IEA:EnsemblFungi.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:1990758; P:mitotic sister chromatid biorientation; IEA:EnsemblFungi.
DR   GO; GO:0051987; P:positive regulation of attachment of spindle microtubules to kinetochore; IEA:EnsemblFungi.
DR   GO; GO:0031116; P:positive regulation of microtubule polymerization; IEA:EnsemblFungi.
DR   InterPro; IPR013966; Spc34.
DR   Pfam; PF08657; DASH_Spc34; 1.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition;
KW   Coiled coil; Cytoplasm; Cytoskeleton; Kinetochore; Microtubule; Mitosis;
KW   Nucleus; Reference proteome.
FT   CHAIN           1..275
FT                   /note="DASH complex subunit SPC34"
FT                   /id="PRO_0000211552"
FT   COILED          203..275
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   275 AA;  31796 MW;  AA9C45436F40AE64 CRC64;
     MSGSLDYCLD QISKSAESIS TLYFKPPGIF RNAIVPGNSK VYSDLIVKLI RDGDSIEEMS
     LYSTDNDGNM KRKDGKVGIY DHLLEREATL KRNRALCLPD PRPITYIPKG FYLSQNDHVI
     RKKQKPARDF IFEGSNSDEL GIYDVLLKKF SKDEQIKQFL HALRNGSVIT GEDVSRRKTL
     FVEDFPISII LSVFREIIDQ WPLTEYKEKF EKLMQIYNGL QADINELQKK VEIQELDFQH
     EALPEKTSIS SLIDKEEKEI QKLEKQLDLL EGRNK