SPC34_SCHPO
ID SPC34_SCHPO Reviewed; 164 AA.
AC O14285;
DT 21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 25-MAY-2022, entry version 114.
DE RecName: Full=DASH complex subunit spc34;
DE AltName: Full=Outer kinetochore protein spc34;
GN Name=spc34; ORFNames=SPAC8C9.17c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=16079914; DOI=10.1038/sj.emboj.7600762;
RA Liu X., McLeod I., Anderson S., Yates J.R. III, He X.;
RT "Molecular analysis of kinetochore architecture in fission yeast.";
RL EMBO J. 24:2919-2930(2005).
CC -!- FUNCTION: Component of the DASH complex, a microtubule-binding
CC subcomplex of the outer kinetochore that is essential for proper
CC chromosome segregation. The DASH complex mediates the formation and
CC maintenance of bipolar kinetochore-microtubule attachments by forming
CC closed rings around spindle microtubules and establishing interactions
CC with proteins from the central kinetochore.
CC {ECO:0000269|PubMed:16079914}.
CC -!- SUBUNIT: The DASH complex oligomerizes to form rings that encircle the
CC microtubules. {ECO:0000269|PubMed:16079914}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16079914}. Cytoplasm,
CC cytoskeleton, spindle {ECO:0000269|PubMed:16079914}. Chromosome,
CC centromere, kinetochore {ECO:0000269|PubMed:16079914}. Note=Associates
CC with the mitotic spindle and the kinetochore. Kinetochore association
CC occurs only during mitosis.
CC -!- SIMILARITY: Belongs to the DASH complex SPC34 family. {ECO:0000305}.
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DR EMBL; CU329670; CAB16304.1; -; Genomic_DNA.
DR PIR; T39153; T39153.
DR RefSeq; NP_594287.1; NM_001019710.2.
DR AlphaFoldDB; O14285; -.
DR SMR; O14285; -.
DR BioGRID; 278578; 188.
DR IntAct; O14285; 1.
DR STRING; 4896.SPAC8C9.17c.1; -.
DR MaxQB; O14285; -.
DR PaxDb; O14285; -.
DR EnsemblFungi; SPAC8C9.17c.1; SPAC8C9.17c.1:pep; SPAC8C9.17c.
DR GeneID; 2542102; -.
DR KEGG; spo:SPAC8C9.17c; -.
DR PomBase; SPAC8C9.17c; spc34.
DR VEuPathDB; FungiDB:SPAC8C9.17c; -.
DR HOGENOM; CLU_1620021_0_0_1; -.
DR InParanoid; O14285; -.
DR OMA; MQGGREY; -.
DR PRO; PR:O14285; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0000779; C:condensed chromosome, centromeric region; IDA:PomBase.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0042729; C:DASH complex; IDA:PomBase.
DR GO; GO:0000776; C:kinetochore; IDA:PomBase.
DR GO; GO:0072686; C:mitotic spindle; HDA:PomBase.
DR GO; GO:0044732; C:mitotic spindle pole body; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0005876; C:spindle microtubule; IEA:InterPro.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:1990758; P:mitotic sister chromatid biorientation; TAS:PomBase.
DR GO; GO:0051455; P:monopolar spindle attachment to meiosis I kinetochore; IC:PomBase.
DR InterPro; IPR013966; Spc34.
DR Pfam; PF08657; DASH_Spc34; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition;
KW Coiled coil; Cytoplasm; Cytoskeleton; Kinetochore; Microtubule; Mitosis;
KW Nucleus; Reference proteome.
FT CHAIN 1..164
FT /note="DASH complex subunit spc34"
FT /id="PRO_0000211553"
FT COILED 104..164
FT /evidence="ECO:0000255"
SQ SEQUENCE 164 AA; 19011 MW; 852BDE9C8896E701 CRC64;
MSDLLNYLQS IAATSEKLLE PENPNAARFT DAVLHTHAIT DLIRDTQKEE LIAAEFKSLP
KDWSERLASE NPADYVACIE ELLDIYPMQG GREYLETLVE KYNLHMSGIE NLENVLLEQK
EQLQQLEKRQ TDQVSARENI LQRETSEIQR LEREIEKVKQ LIQS
