SPC7_SCHPO
ID SPC7_SCHPO Reviewed; 1364 AA.
AC O59757;
DT 12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Kinetochore protein spc7;
DE AltName: Full=NMS complex subunit spc7;
GN Name=spc7; ORFNames=SPCC1020.02;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP IDENTIFICATION IN THE NMS COMPLEX.
RX PubMed=16079914; DOI=10.1038/sj.emboj.7600762;
RA Liu X., McLeod I., Anderson S., Yates J.R. III, He X.;
RT "Molecular analysis of kinetochore architecture in fission yeast.";
RL EMBO J. 24:2919-2930(2005).
RN [3]
RP IDENTIFICATION IN THE NMS COMPLEX.
RX PubMed=17035632; DOI=10.1091/mbc.e06-05-0388;
RA Hayashi A., Asakawa H., Haraguchi T., Hiraoka Y.;
RT "Reconstruction of the kinetochore during meiosis in fission yeast
RT Schizosaccharomyces pombe.";
RL Mol. Biol. Cell 17:5173-5184(2006).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [5]
RP INTERACTION WITH SOS7.
RX PubMed=22711988; DOI=10.1128/mcb.00212-12;
RA Jakopec V., Topolski B., Fleig U.;
RT "Sos7, an essential component of the conserved Schizosaccharomyces pombe
RT Ndc80-MIND-Spc7 complex, identifies a new family of fungal kinetochore
RT proteins.";
RL Mol. Cell. Biol. 32:3308-3320(2012).
CC -!- FUNCTION: Acts as a component of the NMS (Ndc80-MIND-Spc7) super
CC complex which has a role in kinetochore function during late meiotic
CC prophase and throughout the mitotic cell cycle.
CC -!- SUBUNIT: Component of the NMS super complex which consists of mis12,
CC mis13, mis14, ndc80, nnf1, nuf2, sos7, spc7, spc24 and spc25. Interacts
CC (via C-terminus) directly with sos7 (via C-terminus).
CC {ECO:0000269|PubMed:16079914, ECO:0000269|PubMed:17035632,
CC ECO:0000269|PubMed:22711988}.
CC -!- INTERACTION:
CC O59757; Q10113: mal3; NbExp=2; IntAct=EBI-1002205, EBI-1002268;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16823372}.
CC Chromosome, centromere, kinetochore {ECO:0000269|PubMed:16823372}.
CC Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole
CC body {ECO:0000269|PubMed:16823372}.
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DR EMBL; CU329672; CAA18990.1; -; Genomic_DNA.
DR PIR; T40839; T40839.
DR RefSeq; NP_587958.1; NM_001022949.2.
DR AlphaFoldDB; O59757; -.
DR SMR; O59757; -.
DR BioGRID; 275727; 47.
DR DIP; DIP-35340N; -.
DR IntAct; O59757; 5.
DR STRING; 4896.SPCC1020.02.1; -.
DR iPTMnet; O59757; -.
DR PaxDb; O59757; -.
DR PRIDE; O59757; -.
DR EnsemblFungi; SPCC1020.02.1; SPCC1020.02.1:pep; SPCC1020.02.
DR GeneID; 2539155; -.
DR KEGG; spo:SPCC1020.02; -.
DR PomBase; SPCC1020.02; spc7.
DR VEuPathDB; FungiDB:SPCC1020.02; -.
DR eggNOG; ENOG502S20P; Eukaryota.
DR HOGENOM; CLU_256785_0_0_1; -.
DR InParanoid; O59757; -.
DR OMA; RSACHEL; -.
DR PhylomeDB; O59757; -.
DR PRO; PR:O59757; -.
DR Proteomes; UP000002485; Chromosome III.
DR GO; GO:0032153; C:cell division site; HDA:PomBase.
DR GO; GO:0000779; C:condensed chromosome, centromeric region; IDA:PomBase.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0000939; C:inner kinetochore; IDA:PomBase.
DR GO; GO:0000776; C:kinetochore; IDA:PomBase.
DR GO; GO:0031617; C:NMS complex; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0005816; C:spindle pole body; IEA:UniProtKB-SubCell.
DR GO; GO:0140483; F:kinetochore adaptor activity; IPI:PomBase.
DR GO; GO:0051010; F:microtubule plus-end binding; TAS:PomBase.
DR GO; GO:0051315; P:attachment of mitotic spindle microtubules to kinetochore; IMP:PomBase.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:1902426; P:deactivation of mitotic spindle assembly checkpoint; IMP:PomBase.
DR GO; GO:1990813; P:meiotic centromeric cohesion protection; IMP:PomBase.
DR GO; GO:1990758; P:mitotic sister chromatid biorientation; IMP:PomBase.
DR GO; GO:0007094; P:mitotic spindle assembly checkpoint signaling; IMP:PomBase.
DR GO; GO:0034501; P:protein localization to kinetochore; IBA:GO_Central.
DR InterPro; IPR033338; Spc105/Spc7.
DR InterPro; IPR013253; Spc7_domain.
DR PANTHER; PTHR28260; PTHR28260; 1.
DR Pfam; PF08317; Spc7; 1.
DR SMART; SM00787; Spc7; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Coiled coil; Cytoplasm;
KW Cytoskeleton; Kinetochore; Meiosis; Mitosis; Nucleus; Reference proteome.
FT CHAIN 1..1364
FT /note="Kinetochore protein spc7"
FT /id="PRO_0000290644"
FT REGION 1..36
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 124..190
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 202..223
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 289..334
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 456..503
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 564..643
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 697..837
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 1071..1160
FT /evidence="ECO:0000255"
FT COMPBIAS 1..17
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 124..138
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 139..190
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 289..316
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 317..334
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 456..497
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 567..581
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 582..621
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 734..771
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 801..823
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1364 AA; 153593 MW; 829312F81FFB7502 CRC64;
MPTSPRRNSI ATTDNVIGRN KSRKRPHSLG GPGALQELKE HTNPAKGILK SYSSFSVDPA
FTGDEDFNDI HTQINNTVIG ISSNVMAASK RLQMEDLQQL SRETSRKSLN RRVSFASHAR
VRWYPKDHQS DSEKSTSNHS TPERTFASDA KNHSPKGPTT TSFSRNETQS SPHSHSASII
SDGSDMDIAS PIRSTESDMV SEALNAGHPP PSLYPENDDL SIQNPTKALP EAEKALDVHD
ATREQVNDRE ETNMDLTIQF QEADSFLSHS ESIKGLSSSE QGTVYSLKAS HDPSNQTQLS
SPNKSSSPTS IEISDFSKNN ENHDQSENKE EEEDMMLTRP IEIPQHFSPI ARPLTSQEAI
VDMDITSNNI NLSPVSHFSN GLDLQNLEEA PMNLTRPINA NPHLTNHSPN DLTNGEEEMD
TTSAFNIENS HLTLLSPIRP SSRSMEEQIM DLTQPISSTN APTHLNEDDL NQFTSNISSS
SKPRKDNNKT ANSSKPIPDS EDFMDITRPF NILSPSKEAL SEEQPMELTS TVFPCENSTS
HLEVEEAAMD ETVAFQIRGN NVELPSADKE NAEREEIPSY SDKSENFNTT SFTNHERSPN
GNNNLKFSKD PNSSSPSRHV VATPTDKLGT RKRRLRYSTS SFDQSTLRRN RLATIRNARK
SISTLNDREL LPVNFFEKKV NSGLYKSVER SENYRLGATP LTAEKPFTTE KPLSSLPEEV
SRQPTDDKGE QVSNADVDSG LSKTERLTIQ QTNEIKHVPT NTTSSVKLPQ QPSNEDEKER
ITTADYADST SLERLESQEP NRNELVQVGS SNAGNTTSVG MNEHEKSPVK LSKGVSNVDT
SLGASTINTN ILNQDSGPNE EIPVGNEPEF DTMPTLPNVE PISLSDFLKM TGIEFLDNLT
IAKRRETLLP NAEENKKCSI QELLESFYIQ FPLLELYKFS CQQLQDYIAE GKDFVTKIEE
ETLKENPLLF YEYRKASSDM RVLMDSQFLM MKTFARLQAK GDWYEWREGL MQGIKHELNL
NLTGMQRSLT HLMDVANVIH PYAQEIQERY NGSITTVQTL KKQKEFANQY DSTLLAQAQE
KLEKLKVEVE RRRRLLSEKE ERRKELAIKI EQVTNSCSDL ELRTNAEQDF YAKNQDFEFD
EIKRYEEQLL NLKNELGWTI VSLTAGGIKL ATNNTALSPY SAEVTVEILR QNFQVNVDIA
CKFPNESNAC SSNVLEHVAS SFSKWHSKVF SRNLRLLKKY LNDVSICWEQ IVYLVQDFQR
LWYHWPFLSV ENDDKSIIIN VELYLRSVSS KVKVVFGLPI DTIYQTTEVG KFYASTSVAV
KQMYAESEGD SYVSEVLNTL SEVVHCTSTY ALSSACLTVW NKYS
