SPCS1_ARATH
ID SPCS1_ARATH Reviewed; 92 AA.
AC Q944J0; Q7G9Y5;
DT 31-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Signal peptidase complex subunit 1;
DE AltName: Full=Microsomal signal peptidase 12 kDa subunit;
DE Short=SPase 12 kDa subunit;
GN OrderedLocusNames=At2g22425; ORFNames=F14M13.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Component of the signal peptidase complex (SPC) which
CC catalyzes the cleavage of N-terminal signal sequences from nascent
CC proteins as they are translocated into the lumen of the endoplasmic
CC reticulum (By similarity). Dispensable for SPC enzymatic activity (By
CC similarity). {ECO:0000250|UniProtKB:P46965,
CC ECO:0000250|UniProtKB:Q9Y6A9}.
CC -!- SUBUNIT: Component of the signal peptidase complex (SPC) composed of a
CC catalytic subunit SEC11 and three accessory subunits SPCS1, SPCS2 and
CC SPCS3. The complex induces a local thinning of the ER membrane which is
CC used to measure the length of the signal peptide (SP) h-region of
CC protein substrates. This ensures the selectivity of the complex towards
CC h-regions shorter than 18-20 amino acids.
CC {ECO:0000250|UniProtKB:Q9Y6A9}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:P83362}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:P83362}.
CC -!- SIMILARITY: Belongs to the SPCS1 family. {ECO:0000305}.
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DR EMBL; AC006592; AAM15302.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07302.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07303.1; -; Genomic_DNA.
DR EMBL; AF428383; AAL16151.1; -; mRNA.
DR EMBL; AY143801; AAN28740.1; -; mRNA.
DR EMBL; AY087762; AAM65298.1; -; mRNA.
DR RefSeq; NP_001077942.1; NM_001084473.2.
DR RefSeq; NP_565535.1; NM_127807.4.
DR AlphaFoldDB; Q944J0; -.
DR SMR; Q944J0; -.
DR BioGRID; 2127; 172.
DR IntAct; Q944J0; 172.
DR STRING; 3702.AT2G22425.1; -.
DR PaxDb; Q944J0; -.
DR PRIDE; Q944J0; -.
DR ProteomicsDB; 232632; -.
DR EnsemblPlants; AT2G22425.1; AT2G22425.1; AT2G22425.
DR EnsemblPlants; AT2G22425.2; AT2G22425.2; AT2G22425.
DR GeneID; 816774; -.
DR Gramene; AT2G22425.1; AT2G22425.1; AT2G22425.
DR Gramene; AT2G22425.2; AT2G22425.2; AT2G22425.
DR KEGG; ath:AT2G22425; -.
DR Araport; AT2G22425; -.
DR TAIR; locus:505006266; AT2G22425.
DR eggNOG; KOG4112; Eukaryota.
DR HOGENOM; CLU_134505_0_1_1; -.
DR InParanoid; Q944J0; -.
DR OMA; FRAMMLI; -.
DR PhylomeDB; Q944J0; -.
DR PRO; PR:Q944J0; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q944J0; baseline and differential.
DR Genevisible; Q944J0; AT.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0005787; C:signal peptidase complex; IBA:GO_Central.
DR GO; GO:0045047; P:protein targeting to ER; IBA:GO_Central.
DR GO; GO:0006465; P:signal peptide processing; IBA:GO_Central.
DR InterPro; IPR009542; Spc1/SPCS1.
DR PANTHER; PTHR13202; PTHR13202; 1.
DR Pfam; PF06645; SPC12; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..92
FT /note="Signal peptidase complex subunit 1"
FT /id="PRO_0000215160"
FT TOPO_DOM 1..12
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:P83362"
FT TRANSMEM 13..30
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 31..36
FT /note="Lumenal"
FT /evidence="ECO:0000250|UniProtKB:P83362"
FT TRANSMEM 37..59
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 60..92
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:P83362"
FT REGION 73..92
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 92 AA; 10373 MW; E626DBFBA6D03587 CRC64;
MDWQGQKLVE QLMQILLVIS GVVAVVVGYT TESFRTMMLI YAGGVVLTTL VTVPNWPFYN
LHPLKWLDPS EAEKHPKPEV VSVASKKKFS KK
