SPCS3_RAT
ID SPCS3_RAT Reviewed; 180 AA.
AC Q568Z4;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Signal peptidase complex subunit 3;
DE AltName: Full=Microsomal signal peptidase 22/23 kDa subunit;
DE Short=SPC22/23;
DE Short=SPase 22/23 kDa subunit;
GN Name=Spcs3;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Essential component of the signal peptidase complex (SPC)
CC which catalyzes the cleavage of N-terminal signal sequences from
CC nascent proteins as they are translocated into the lumen of the
CC endoplasmic reticulum (By similarity). Essential for the SPC catalytic
CC activity, possibly by stabilizing and positioning the active center of
CC the complex close to the lumenal surface (By similarity).
CC {ECO:0000250|UniProtKB:P61009, ECO:0000250|UniProtKB:Q12133}.
CC -!- SUBUNIT: Component of the signal peptidase complex paralog A (SPC-A)
CC composed of a catalytic subunit SEC11A and three accessory subunits
CC SPCS1, SPCS2 and SPCS3. Component of the signal peptidase complex
CC paralog C (SPC-C) composed of a catalytic subunit SEC11C and three
CC accessory subunits SPCS1, SPCS2 and SPCS3. Within the complex,
CC interacts with SEC11A or SEC11C and SPCS1. The complex induces a local
CC thinning of the ER membrane which is used to measure the length of the
CC signal peptide (SP) h-region of protein substrates. This ensures the
CC selectivity of the complex towards h-regions shorter than 18-20 amino
CC acids. {ECO:0000250|UniProtKB:P61009}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:P61008}; Single-pass type II membrane protein
CC {ECO:0000250|UniProtKB:P61008}.
CC -!- SIMILARITY: Belongs to the SPCS3 family. {ECO:0000305}.
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DR EMBL; BC092634; AAH92634.1; -; mRNA.
DR RefSeq; NP_001019438.1; NM_001024267.2.
DR RefSeq; XP_006227529.1; XM_006227467.2.
DR RefSeq; XP_006257345.1; XM_006257283.3.
DR AlphaFoldDB; Q568Z4; -.
DR SMR; Q568Z4; -.
DR IntAct; Q568Z4; 2.
DR STRING; 10116.ENSRNOP00000064651; -.
DR GlyGen; Q568Z4; 1 site.
DR PaxDb; Q568Z4; -.
DR PRIDE; Q568Z4; -.
DR Ensembl; ENSRNOT00000070802; ENSRNOP00000066197; ENSRNOG00000049355.
DR Ensembl; ENSRNOT00000075521; ENSRNOP00000064651; ENSRNOG00000046858.
DR GeneID; 100360464; -.
DR GeneID; 317409; -.
DR KEGG; rno:100360464; -.
DR KEGG; rno:317409; -.
DR UCSC; RGD:1563536; rat.
DR CTD; 76976; -.
DR RGD; 1563536; MGC109340.
DR eggNOG; KOG3372; Eukaryota.
DR GeneTree; ENSGT00390000009223; -.
DR HOGENOM; CLU_068714_1_0_1; -.
DR InParanoid; Q568Z4; -.
DR OMA; TLCLFNY; -.
DR OrthoDB; 1514162at2759; -.
DR PhylomeDB; Q568Z4; -.
DR TreeFam; TF300185; -.
DR PRO; PR:Q568Z4; -.
DR Proteomes; UP000002494; Chromosome X.
DR Bgee; ENSRNOG00000046858; Expressed in cerebellum and 17 other tissues.
DR Genevisible; Q568Z4; RN.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005787; C:signal peptidase complex; IBA:GO_Central.
DR GO; GO:0045047; P:protein targeting to ER; IBA:GO_Central.
DR GO; GO:0006465; P:signal peptide processing; IBA:GO_Central.
DR InterPro; IPR007653; SPC3.
DR PANTHER; PTHR12804; PTHR12804; 1.
DR Pfam; PF04573; SPC22; 1.
DR PIRSF; PIRSF016089; SPC22; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Glycoprotein; Membrane; Reference proteome;
KW Signal-anchor; Transmembrane; Transmembrane helix.
FT CHAIN 1..180
FT /note="Signal peptidase complex subunit 3"
FT /id="PRO_0000218940"
FT TOPO_DOM 1..11
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:P61008"
FT TRANSMEM 12..32
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 33..180
FT /note="Lumenal"
FT /evidence="ECO:0000250|UniProtKB:P61008"
FT CARBOHYD 141
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 180 AA; 20160 MW; 0E82F043A44DAAEE CRC64;
MNTLLSRANS LFAFTLSVMA ALTLGCILTT AFKDRSAPVR LHVSRILLKK VEDFTGPRKK
SDLGFITFHI SADLEKTFDW NVKQLFLYLS AEYSTKSNAV NQVVLWDKIL LRGENPKLNL
KDVKSKYFFF DDGHGLKGNR NVTLTLSWQV IPIAGILPLV TGSGRVSVPF PDSYEIATTF
