ID   SPD1_TRICX              Reviewed;         748 AA.
AC   P19837;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   05-OCT-2010, sequence version 3.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Spidroin-1;
DE   AltName: Full=Dragline silk fibroin 1;
DE   Flags: Fragment;
OS   Trichonephila clavipes (Golden silk orbweaver) (Nephila clavipes).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Araneoidea; Nephilidae; Trichonephila.
OX   NCBI_TaxID=2585209;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RX   PubMed=2402494; DOI=10.1073/pnas.87.18.7120;
RA   Xu M., Lewis R.V.;
RT   "Structure of a protein superfiber: spider dragline silk.";
RL   Proc. Natl. Acad. Sci. U.S.A. 87:7120-7124(1990).
RN   [2]
RP   SEQUENCE REVISION TO C-TERMINUS.
RA   Xu M., Lewis R.V.;
RL   Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE OF 653-747.
RX   PubMed=8120021; DOI=10.1016/s0021-9258(17)37425-2;
RA   Beckwitt R., Arcidiacono S.;
RT   "Sequence conservation in the C-terminal region of spider silk proteins
RT   (Spidroin) from Nephila clavipes (Tetragnathidae) and Araneus bicentenarius
RT   (Araneidae).";
RL   J. Biol. Chem. 269:6661-6663(1994).
CC   -!- FUNCTION: Spiders' major ampullate silk possesses unique
CC       characteristics of strength and elasticity. Fibroin consists of
CC       pseudocrystalline regions of antiparallel beta-sheet interspersed with
CC       elastic amorphous segments.
CC   -!- SUBUNIT: Major subunit, with spidroin 2, of the dragline silk.
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space.
CC   -!- DOMAIN: Highly repetitive protein characterized by regions of
CC       polyalanine and glycine-rich repeating units.
CC   -!- SIMILARITY: Belongs to the silk fibroin family. {ECO:0000305}.
CC   -!- WEB RESOURCE: Name=Protein Spotlight; Note=The tiptoe of an airbus
CC       - Issue 24 of July 2002;
CC       URL="https://web.expasy.org/spotlight/back_issues/024";
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DR   EMBL; M37137; AAA29380.2; -; mRNA.
DR   EMBL; U03848; AAB60212.1; -; Unassigned_DNA.
DR   PIR; A36068; A36068.
DR   AlphaFoldDB; P19837; -.
DR   PCDDB; P19837; -.
DR   SMR; P19837; -.
DR   PRIDE; P19837; -.
DR   GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR   Gene3D; 1.10.10.1350; -; 1.
DR   InterPro; IPR021001; Spidroin_C.
DR   InterPro; IPR038542; Spidroin_C_sf.
DR   Pfam; PF11260; Spidroin_MaSp; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Repeat; Secreted; Silk protein.
FT   CHAIN           <1..748
FT                   /note="Spidroin-1"
FT                   /id="PRO_0000221446"
FT   REPEAT          1..25
FT                   /note="1"
FT   REPEAT          26..38
FT                   /note="2"
FT   REPEAT          39..66
FT                   /note="3"
FT   REPEAT          67..96
FT                   /note="4"
FT   REPEAT          97..130
FT                   /note="5"
FT   REPEAT          131..158
FT                   /note="6"
FT   REPEAT          159..191
FT                   /note="7"
FT   REPEAT          192..204
FT                   /note="8"
FT   REPEAT          205..235
FT                   /note="9"
FT   REPEAT          236..262
FT                   /note="10"
FT   REPEAT          263..292
FT                   /note="11"
FT   REPEAT          293..305
FT                   /note="12"
FT   REPEAT          306..333
FT                   /note="13"
FT   REPEAT          334..360
FT                   /note="14"
FT   REPEAT          361..394
FT                   /note="15"
FT   REPEAT          395..424
FT                   /note="16"
FT   REPEAT          425..458
FT                   /note="17"
FT   REPEAT          459..485
FT                   /note="18"
FT   REPEAT          486..512
FT                   /note="19"
FT   REPEAT          513..525
FT                   /note="20"
FT   REPEAT          526..555
FT                   /note="21"
FT   REPEAT          556..582
FT                   /note="22"
FT   REPEAT          583..612
FT                   /note="23"
FT   REPEAT          613..642
FT                   /note="24"
FT   REPEAT          643..655
FT                   /note="25"
FT   REGION          1..655
FT                   /note="25 X approximate tandem repeats"
FT   CONFLICT        662
FT                   /note="V -> L (in Ref. 1; AAA29380)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        672
FT                   /note="S -> T (in Ref. 1; AAA29380)"
FT                   /evidence="ECO:0000305"
FT   NON_TER         1
SQ   SEQUENCE   748 AA;  60585 MW;  70F50E44B0D649E0 CRC64;
     QGAGAAAAAA GGAGQGGYGG LGGQGAGQGG YGGLGGQGAG QGAGAAAAAA AGGAGQGGYG
     GLGSQGAGRG GQGAGAAAAA AGGAGQGGYG GLGSQGAGRG GLGGQGAGAA AAAAAGGAGQ
     GGYGGLGNQG AGRGGQGAAA AAAGGAGQGG YGGLGSQGAG RGGLGGQGAG AAAAAAGGAG
     QGGYGGLGGQ GAGQGGYGGL GSQGAGRGGL GGQGAGAAAA AAAGGAGQGG LGGQGAGQGA
     GASAAAAGGA GQGGYGGLGS QGAGRGGEGA GAAAAAAGGA GQGGYGGLGG QGAGQGGYGG
     LGSQGAGRGG LGGQGAGAAA AGGAGQGGLG GQGAGQGAGA AAAAAGGAGQ GGYGGLGSQG
     AGRGGLGGQG AGAVAAAAAG GAGQGGYGGL GSQGAGRGGQ GAGAAAAAAG GAGQRGYGGL
     GNQGAGRGGL GGQGAGAAAA AAAGGAGQGG YGGLGNQGAG RGGQGAAAAA GGAGQGGYGG
     LGSQGAGRGG QGAGAAAAAA VGAGQEGIRG QGAGQGGYGG LGSQGSGRGG LGGQGAGAAA
     AAAGGAGQGG LGGQGAGQGA GAAAAAAGGV RQGGYGGLGS QGAGRGGQGA GAAAAAAGGA
     GQGGYGGLGG QGVGRGGLGG QGAGAAAAGG AGQGGYGGVG SGASAASAAA SRLSSPQASS
     RVSSAVSNLV ASGPTNSAAL SSTISNVVSQ IGASNPGLSG CDVLIQALLE VVSALIQILG
     SSSIGQVNYG SAGQATQIVG QSVYQALG