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SPD2A_MOUSE
ID   SPD2A_MOUSE             Reviewed;        1124 AA.
AC   O89032; E9QKJ2; Q148Q8;
DT   20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   27-JUL-2011, sequence version 2.
DT   03-AUG-2022, entry version 162.
DE   RecName: Full=SH3 and PX domain-containing protein 2A;
DE   AltName: Full=Five SH3 domain-containing protein;
DE   AltName: Full=SH3 multiple domains protein 1;
DE   AltName: Full=Tyrosine kinase substrate with five SH3 domains;
GN   Name=Sh3pxd2a; Synonyms=Fish, Sh3md1, Tks5;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING (ISOFORM 3),
RP   PTM, AND TISSUE SPECIFICITY.
RX   PubMed=9687503; DOI=10.1093/emboj/17.15.4346;
RA   Lock P., Abram C.L., Gibson T., Courtneidge S.A.;
RT   "A new method for isolating tyrosine kinase substrates used to identify
RT   fish, an SH3 and PX domain-containing protein, and Src substrate.";
RL   EMBO J. 17:4346-4357(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-812, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Liver;
RX   PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA   Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT   "Large-scale phosphorylation analysis of mouse liver.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN   [5]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=18417249; DOI=10.1016/j.ejcb.2008.02.008;
RA   Blouw B., Seals D.F., Pass I., Diaz B., Courtneidge S.A.;
RT   "A role for the podosome/invadopodia scaffold protein Tks5 in tumor growth
RT   in vivo.";
RL   Eur. J. Cell Biol. 87:555-567(2008).
RN   [6]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND INTERACTION WITH CYBA.
RX   PubMed=19755709; DOI=10.1126/scisignal.2000368;
RA   Diaz B., Shani G., Pass I., Anderson D., Quintavalle M., Courtneidge S.A.;
RT   "Tks5-dependent, nox-mediated generation of reactive oxygen species is
RT   necessary for invadopodia formation.";
RL   Sci. Signal. 2:RA53-RA53(2009).
RN   [7]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-256; SER-546; SER-643;
RP   SER-764; SER-766; SER-812; THR-822; SER-993; SER-1007; SER-1008 AND
RP   SER-1029, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC   and Spleen;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Adapter protein involved in invadopodia and podosome
CC       formation, extracellular matrix degradation and invasiveness of some
CC       cancer cells. Binds matrix metalloproteinases (ADAMs), NADPH oxidases
CC       (NOXs) and phosphoinositides. Acts as an organizer protein that allows
CC       NOX1- or NOX3-dependent reactive oxygen species (ROS) generation and
CC       ROS localization. In association with ADAM12, mediates the neurotoxic
CC       effect of amyloid-beta peptide (By similarity). {ECO:0000250,
CC       ECO:0000269|PubMed:18417249, ECO:0000269|PubMed:19755709}.
CC   -!- SUBUNIT: Interacts with ADAM12, ADAM15 and ADAM19 (By similarity).
CC       Interacts with NOXO1 (By similarity). Interacts (via SH3 domains) with
CC       NOXA1; the interaction is direct (By similarity). Interacts (via N-
CC       terminus) with CYBA. Interacts with FASLG (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Cell projection, podosome
CC       {ECO:0000250}. Note=Cytoplasmic in normal cells and localizes to
CC       podosomes in Src-transformed cells. {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC         Comment=Additional isoforms seem to exist.;
CC       Name=1;
CC         IsoId=O89032-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=O89032-2; Sequence=VSP_023315;
CC       Name=3;
CC         IsoId=O89032-3; Sequence=VSP_023314, VSP_023315;
CC   -!- TISSUE SPECIFICITY: Widely expressed. Not found in the spleen and
CC       testis. {ECO:0000269|PubMed:9687503}.
CC   -!- DOMAIN: The PX domain is required for podosome localization because of
CC       its ability to bind phosphatidylinositol 3-phosphate (PtdIns(3)P) and
CC       phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) and, to a lesser
CC       extent, phosphatidylinositol 4-phosphate (PtdIns(4)P),
CC       phosphatidylinositol 5-phosphate (PtdIns(5)P), and phosphatidylinositol
CC       3,5-bisphosphate (PtdIns(3,5)P2). Binds to the third intramolecular SH3
CC       domain (By similarity). {ECO:0000250}.
CC   -!- DOMAIN: The fifth SH3 domain mediates binding with ADAM12, ADAM15 and
CC       ADAM19. {ECO:0000250}.
CC   -!- PTM: Tyrosine phosphorylated by SRC. Phosphorylation plays a regulatory
CC       role in the protein localization. The intramolecular interaction of the
CC       PX domain with the third SH3 domain maintains the protein in the
CC       cytoplasm and phosphorylation disrupts this interaction, resulting in
CC       the redistribution of the protein from cytoplasm to the perimembrane
CC       region. Phosphorylated on serine upon DNA damage, probably by ATM or
CC       ATR (By similarity). {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: Shows significant decrease in total cellular
CC       reactive oxygen species (ROS) and in podosome formation.
CC       {ECO:0000269|PubMed:18417249, ECO:0000269|PubMed:19755709}.
CC   -!- SIMILARITY: Belongs to the SH3PXD2 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAI18023.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AJ007012; CAA07416.1; -; mRNA.
DR   EMBL; AC132268; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC132288; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC118022; AAI18023.1; ALT_FRAME; mRNA.
DR   CCDS; CCDS29891.1; -. [O89032-1]
DR   CCDS; CCDS50464.1; -. [O89032-2]
DR   RefSeq; NP_001158189.1; NM_001164717.1. [O89032-2]
DR   RefSeq; NP_032044.2; NM_008018.4. [O89032-1]
DR   AlphaFoldDB; O89032; -.
DR   SMR; O89032; -.
DR   BioGRID; 199678; 5.
DR   IntAct; O89032; 2.
DR   MINT; O89032; -.
DR   STRING; 10090.ENSMUSP00000080325; -.
DR   iPTMnet; O89032; -.
DR   PhosphoSitePlus; O89032; -.
DR   EPD; O89032; -.
DR   jPOST; O89032; -.
DR   MaxQB; O89032; -.
DR   PaxDb; O89032; -.
DR   PeptideAtlas; O89032; -.
DR   PRIDE; O89032; -.
DR   ProteomicsDB; 261123; -. [O89032-1]
DR   ProteomicsDB; 261124; -. [O89032-2]
DR   ProteomicsDB; 261125; -. [O89032-3]
DR   Antibodypedia; 46067; 180 antibodies from 32 providers.
DR   DNASU; 14218; -.
DR   Ensembl; ENSMUST00000081619; ENSMUSP00000080325; ENSMUSG00000053617. [O89032-1]
DR   Ensembl; ENSMUST00000111800; ENSMUSP00000107430; ENSMUSG00000053617. [O89032-2]
DR   GeneID; 14218; -.
DR   KEGG; mmu:14218; -.
DR   UCSC; uc008huy.2; mouse. [O89032-1]
DR   UCSC; uc008hva.2; mouse. [O89032-2]
DR   CTD; 9644; -.
DR   MGI; MGI:1298393; Sh3pxd2a.
DR   VEuPathDB; HostDB:ENSMUSG00000053617; -.
DR   eggNOG; KOG0905; Eukaryota.
DR   GeneTree; ENSGT00940000157732; -.
DR   HOGENOM; CLU_013051_0_0_1; -.
DR   InParanoid; O89032; -.
DR   OMA; TKEECIY; -.
DR   OrthoDB; 1183210at2759; -.
DR   PhylomeDB; O89032; -.
DR   TreeFam; TF329347; -.
DR   Reactome; R-MMU-8941237; Invadopodia formation.
DR   Reactome; R-MMU-9013148; CDC42 GTPase cycle.
DR   BioGRID-ORCS; 14218; 1 hit in 71 CRISPR screens.
DR   ChiTaRS; Sh3pxd2a; mouse.
DR   PRO; PR:O89032; -.
DR   Proteomes; UP000000589; Chromosome 19.
DR   RNAct; O89032; protein.
DR   Bgee; ENSMUSG00000053617; Expressed in molar tooth and 217 other tissues.
DR   ExpressionAtlas; O89032; baseline and differential.
DR   Genevisible; O89032; MM.
DR   GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR   GO; GO:0042995; C:cell projection; IEA:UniProtKB-KW.
DR   GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR   GO; GO:0002102; C:podosome; IDA:UniProtKB.
DR   GO; GO:0035091; F:phosphatidylinositol binding; IMP:UniProtKB.
DR   GO; GO:0043325; F:phosphatidylinositol-3,4-bisphosphate binding; IMP:UniProtKB.
DR   GO; GO:0032266; F:phosphatidylinositol-3-phosphate binding; IMP:UniProtKB.
DR   GO; GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; IMP:UniProtKB.
DR   GO; GO:0070273; F:phosphatidylinositol-4-phosphate binding; IMP:UniProtKB.
DR   GO; GO:0010314; F:phosphatidylinositol-5-phosphate binding; IMP:UniProtKB.
DR   GO; GO:0002020; F:protease binding; IPI:UniProtKB.
DR   GO; GO:0016176; F:superoxide-generating NADPH oxidase activator activity; IBA:GO_Central.
DR   GO; GO:0001701; P:in utero embryonic development; IMP:MGI.
DR   GO; GO:0072675; P:osteoclast fusion; ISO:MGI.
DR   GO; GO:0072593; P:reactive oxygen species metabolic process; IMP:UniProtKB.
DR   GO; GO:0042554; P:superoxide anion generation; IBA:GO_Central.
DR   GO; GO:0006801; P:superoxide metabolic process; ISO:MGI.
DR   CDD; cd06888; PX_FISH; 1.
DR   CDD; cd12074; SH3_Tks5_1; 1.
DR   CDD; cd12077; SH3_Tks5_2; 1.
DR   CDD; cd12079; SH3_Tks5_3; 1.
DR   CDD; cd12019; SH3_Tks5_4; 1.
DR   CDD; cd12020; SH3_Tks5_5; 1.
DR   Gene3D; 3.30.1520.10; -; 1.
DR   InterPro; IPR001683; PX_dom.
DR   InterPro; IPR036871; PX_dom_sf.
DR   InterPro; IPR036028; SH3-like_dom_sf.
DR   InterPro; IPR001452; SH3_domain.
DR   InterPro; IPR037961; SH3PXD2_PX.
DR   InterPro; IPR034917; SH3PXD2A.
DR   InterPro; IPR035450; SH3PXD2A_SH3_1.
DR   InterPro; IPR035452; SH3PXD2A_SH3_2.
DR   InterPro; IPR035449; SH3PXD2A_SH3_3.
DR   InterPro; IPR035453; SH3PXD2A_SH3_4.
DR   InterPro; IPR035454; SH3PXD2A_SH3_5.
DR   PANTHER; PTHR15706:SF22; PTHR15706:SF22; 1.
DR   Pfam; PF00787; PX; 1.
DR   Pfam; PF00018; SH3_1; 4.
DR   SMART; SM00312; PX; 1.
DR   SMART; SM00326; SH3; 5.
DR   SUPFAM; SSF50044; SSF50044; 5.
DR   SUPFAM; SSF64268; SSF64268; 1.
DR   PROSITE; PS50195; PX; 1.
DR   PROSITE; PS50002; SH3; 5.
PE   1: Evidence at protein level;
KW   Alternative splicing; Cell junction; Cell projection; Coiled coil;
KW   Cytoplasm; Phosphoprotein; Reference proteome; Repeat; SH3 domain.
FT   CHAIN           1..1124
FT                   /note="SH3 and PX domain-containing protein 2A"
FT                   /id="PRO_0000278489"
FT   DOMAIN          4..128
FT                   /note="PX"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00147"
FT   DOMAIN          166..225
FT                   /note="SH3 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00192"
FT   DOMAIN          266..325
FT                   /note="SH3 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00192"
FT   DOMAIN          447..506
FT                   /note="SH3 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00192"
FT   DOMAIN          833..892
FT                   /note="SH3 4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00192"
FT   DOMAIN          1063..1124
FT                   /note="SH3 5"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00192"
FT   REGION          414..443
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          504..672
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          692..830
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          886..952
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1020..1050
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          907..937
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        571..585
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        631..671
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        692..716
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        720..737
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        759..780
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        890..905
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        906..922
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         256
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         405
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q5TCZ1"
FT   MOD_RES         420
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q5TCZ1"
FT   MOD_RES         546
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         566
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q5TCZ1"
FT   MOD_RES         592
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q5TCZ1"
FT   MOD_RES         643
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         728
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:Q5TCZ1"
FT   MOD_RES         764
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         766
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         812
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:17242355,
FT                   ECO:0007744|PubMed:21183079"
FT   MOD_RES         822
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         993
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         1007
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         1008
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         1029
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   VAR_SEQ         143..157
FT                   /note="Missing (in isoform 3)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_023314"
FT   VAR_SEQ         240..267
FT                   /note="Missing (in isoform 2 and isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_023315"
FT   CONFLICT        867
FT                   /note="E -> V (in Ref. 1; CAA07416)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1124 AA;  124201 MW;  2A06118D1CE7C567 CRC64;
     MLAYCVQDAT VVDVEKRRSP SKHYVYIINV TWSDSTSQTI YRRYSKFFDL QMQLLDKFPI
     EGGQKDPKQR IIPFLPGKIL FRRSHIRDVA VKRLKPIDEY CRALVRLPPH ISQCDEVFRF
     FEARPEDVNP PKEDYGSSKR KSVWLSSWAE SPKKDVTGAD TNAEPMILEQ YVVVSNYKKQ
     ENSELSLQAG EVVDVIEKNE SGWWFVSTSE EQGWVPATYL EAQNGTRDDS DINTSKTGEV
     SKRRKAHLRR LDRRWTLGGM VNRQHSREEK YVTVQPYTSQ SKDEIGFEKG VTVEVIRKNL
     EGWWYIRYLG KEGWAPASYL KKAKDDLPTR KKNLAGPVEI IGNIMEISNL LNKKASGDKE
     APAEGEGSEA PITKKEISLP ILCNASNGSA LAIPERTTSK LAQGSPAVAR IAPQRAQISS
     PNLRTRPPPR RESSLGFQLP KPPEPPSVEV EYYTIAEFQS CISDGISFRG GQKAEVIDKN
     SGGWWYVQIG EKEGWAPASY IDKRKKPNLS RRTSTLTRPK VPPPAPPSKP KEAEENPVGA
     CESQGSPLKV KYEEPEYDVP AFGFDSEPEM NEEPSGDRGS GDKHPAQPRR ISPASSLQRA
     HFKVGESSED VALEEETIYE NEGFRPYTED TLSARGSSGD SDSPGSSSLS LAVKNSPKSD
     SPKSSSLLKL KAEKNAQAEL GKNQSNISFS SSVTISTTCS SSSSSSSLSK NNGDLKPRSA
     SDAGIRDTPK VGTKKDPDVK AGLASCARAK PSVRPKPVLN RAESQSQEKM DISSLRRQLR
     PTGQLRGGLK GSRSEDSELP PQMASEGSRR GSADIIPLTA TTPPCVPKKE WEGQGATYVT
     CSAYQKVQDS EISFPEGAEV HVLEKAESGW WYVRFGELEG WAPSHYLVAE ENQQPDTASK
     EGDTGKSSQN EGKSDSLEKI EKRVQALNTV NQSKRATPPI PSKPPGGFGK TSGTVAVKMR
     NGVRQVAVRP QSVFVSPPPK DNNLSCALRR NESLTATDSL RGVRRNSSFS TARSAAAEAK
     GRLAERAASQ GSESPLLPTQ RKGIPVSPVR PKPIEKSQFI HNNLKDVYIS IADYEGDEET
     AGFQEGVSME VLEKNPNGWW YCQILDEVKP FKGWVPSNYL EKKN
 
 
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