ID   SPD2_TRICX              Reviewed;         627 AA.
AC   P46804;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 61.
DE   RecName: Full=Spidroin-2;
DE   AltName: Full=Dragline silk fibroin 2;
DE   Flags: Fragment;
OS   Trichonephila clavipes (Golden silk orbweaver) (Nephila clavipes).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Araneoidea; Nephilidae; Trichonephila.
OX   NCBI_TaxID=2585209;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=1527052; DOI=10.1016/s0021-9258(18)41777-2;
RA   Hinman M.B., Lewis R.V.;
RT   "Isolation of a clone encoding a second dragline silk fibroin. Nephila
RT   clavipes dragline silk is a two-protein fiber.";
RL   J. Biol. Chem. 267:19320-19324(1992).
CC   -!- FUNCTION: Spiders' major ampullate silk possesses unique
CC       characteristics of strength and elasticity. Fibroin consists of
CC       pseudocrystalline regions of antiparallel beta-sheet interspersed with
CC       elastic amorphous segments.
CC   -!- SUBUNIT: Major subunit, with spidroin 1, of the dragline silk.
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space.
CC   -!- DOMAIN: Highly repetitive protein characterized by regions of
CC       polyalanine and glycine-rich repeating units.
CC   -!- SIMILARITY: Belongs to the silk fibroin family. {ECO:0000305}.
CC   -!- WEB RESOURCE: Name=Protein Spotlight; Note=The tiptoe of an airbus
CC       - Issue 24 of July 2002;
CC       URL="https://web.expasy.org/spotlight/back_issues/024";
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DR   EMBL; M92913; AAA29381.1; -; mRNA.
DR   PIR; A44112; A44112.
DR   AlphaFoldDB; P46804; -.
DR   SMR; P46804; -.
DR   GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR   Gene3D; 1.10.10.1350; -; 1.
DR   InterPro; IPR021001; Spidroin_C.
DR   InterPro; IPR038542; Spidroin_C_sf.
DR   Pfam; PF11260; Spidroin_MaSp; 1.
PE   2: Evidence at transcript level;
KW   Repeat; Secreted; Silk protein.
FT   CHAIN           <1..627
FT                   /note="Spidroin-2"
FT                   /id="PRO_0000221447"
FT   REPEAT          1..36
FT                   /note="1"
FT   REPEAT          37..79
FT                   /note="2"
FT   REPEAT          80..121
FT                   /note="3"
FT   REPEAT          122..172
FT                   /note="4"
FT   REPEAT          173..213
FT                   /note="5"
FT   REPEAT          214..252
FT                   /note="6"
FT   REPEAT          253..283
FT                   /note="7"
FT   REPEAT          284..317
FT                   /note="8"
FT   REPEAT          318..359
FT                   /note="9"
FT   REPEAT          360..391
FT                   /note="10"
FT   REPEAT          392..428
FT                   /note="11"
FT   REPEAT          429..464
FT                   /note="12"
FT   REPEAT          465..488
FT                   /note="13"
FT   REPEAT          489..515
FT                   /note="14"
FT   REPEAT          516..530
FT                   /note="15"
FT   REGION          1..530
FT                   /note="15 X approximate tandem repeats"
FT   REGION          1..508
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   NON_TER         1
SQ   SEQUENCE   627 AA;  54184 MW;  CB9B63779B2C594B CRC64;
     PGGYGPGQQG PGGYGPGQQG PSGPGSAAAA AAAAAAGPGG YGPGQQGPGG YGPGQQGPGR
     YGPGQQGPSG PGSAAAAAAG SGQQGPGGYG PRQQGPGGYG QGQQGPSGPG SAAAASAAAS
     AESGQQGPGG YGPGQQGPGG YGPGQQGPGG YGPGQQGPSG PGSAAAAAAA ASGPGQQGPG
     GYGPGQQGPG GYGPGQQGPS GPGSAAAAAA AASGPGQQGP GGYGPGQQGP GGYGPGQQGL
     SGPGSAAAAA AAGPGQQGPG GYGPGQQGPS GPGSAAAAAA AAAGPGGYGP GQQGPGGYGP
     GQQGPSGAGS AAAAAAAGPG QQGLGGYGPG QQGPGGYGPG QQGPGGYGPG SASAAAAAAG
     PGQQGPGGYG PGQQGPSGPG SASAAAAAAA AGPGGYGPGQ QGPGGYAPGQ QGPSGPGSAS
     AAAAAAAAGP GGYGPGQQGP GGYAPGQQGP SGPGSAAAAA AAAAGPGGYG PAQQGPSGPG
     IAASAASAGP GGYGPAQQGP AGYGPGSAVA ASAGAGSAGY GPGSQASAAA SRLASPDSGA
     RVASAVSNLV SSGPTSSAAL SSVISNAVSQ IGASNPGLSG CDVLIQALLE IVSACVTILS
     SSSIGQVNYG AASQFAQVVG QSVLSAF