SPDEF_MOUSE
ID SPDEF_MOUSE Reviewed; 325 AA.
AC Q9WTP3;
DT 21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=SAM pointed domain-containing Ets transcription factor;
DE AltName: Full=Prostate epithelium-specific Ets transcription factor;
DE Short=Prostate-specific Ets;
DE AltName: Full=Prostate-derived Ets factor;
GN Name=Spdef; Synonyms=Pdef, Pse;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=C57BL/6J; TISSUE=Colon;
RX PubMed=10675039; DOI=10.1016/s0378-1119(99)00484-9;
RA Yamada N., Tamai Y., Miyamoto H., Nozaki M.;
RT "Cloning and expression of the mouse Pse gene encoding a novel Ets family
RT member.";
RL Gene 241:267-274(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Colon;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: May function as an androgen-independent transactivator of the
CC prostate-specific antigen (PSA) promoter. Binds to 5'-GGAT-3' DNA
CC sequences. May play a role in the regulation of the prostate gland
CC and/or prostate cancer development. Acts as a transcriptional activator
CC for SERPINB5 promoter (By similarity). {ECO:0000250,
CC ECO:0000269|PubMed:10675039}.
CC -!- SUBUNIT: Interacts with the DNA-binding domain of the androgen
CC receptor. Interacts with NKX3-1 (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in the accessory glands of sex organs
CC including the prostate, seminal vesicle, coagulating gland in males,
CC the oviduct in females, and in intestines. Expression is epithelial-
CC specific. {ECO:0000269|PubMed:10675039}.
CC -!- SIMILARITY: Belongs to the ETS family. {ECO:0000305}.
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DR EMBL; AB019436; BAA77329.1; -; mRNA.
DR EMBL; AK078863; BAC37426.1; -; mRNA.
DR EMBL; BC012648; AAH12648.1; -; mRNA.
DR CCDS; CCDS28568.1; -.
DR RefSeq; NP_038919.1; NM_013891.4.
DR RefSeq; XP_006524442.1; XM_006524379.3.
DR RefSeq; XP_006524443.1; XM_006524380.3.
DR RefSeq; XP_006524444.1; XM_006524381.3.
DR RefSeq; XP_017172988.1; XM_017317499.1.
DR AlphaFoldDB; Q9WTP3; -.
DR SMR; Q9WTP3; -.
DR BioGRID; 205953; 2.
DR IntAct; Q9WTP3; 1.
DR STRING; 10090.ENSMUSP00000110520; -.
DR PhosphoSitePlus; Q9WTP3; -.
DR PaxDb; Q9WTP3; -.
DR PRIDE; Q9WTP3; -.
DR ProteomicsDB; 261127; -.
DR Antibodypedia; 15077; 232 antibodies from 30 providers.
DR DNASU; 30051; -.
DR Ensembl; ENSMUST00000025054; ENSMUSP00000025054; ENSMUSG00000024215.
DR Ensembl; ENSMUST00000167489; ENSMUSP00000127056; ENSMUSG00000024215.
DR GeneID; 30051; -.
DR KEGG; mmu:30051; -.
DR UCSC; uc008bpo.2; mouse.
DR CTD; 25803; -.
DR MGI; MGI:1353422; Spdef.
DR VEuPathDB; HostDB:ENSMUSG00000024215; -.
DR eggNOG; KOG3805; Eukaryota.
DR GeneTree; ENSGT00940000157549; -.
DR HOGENOM; CLU_877059_0_0_1; -.
DR InParanoid; Q9WTP3; -.
DR OMA; TWVAKGP; -.
DR OrthoDB; 908495at2759; -.
DR PhylomeDB; Q9WTP3; -.
DR TreeFam; TF318679; -.
DR BioGRID-ORCS; 30051; 3 hits in 76 CRISPR screens.
DR PRO; PR:Q9WTP3; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q9WTP3; protein.
DR Bgee; ENSMUSG00000024215; Expressed in crypt of Lieberkuhn of small intestine and 78 other tissues.
DR ExpressionAtlas; Q9WTP3; baseline and differential.
DR Genevisible; Q9WTP3; MM.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISO:MGI.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0045165; P:cell fate commitment; IMP:MGI.
DR GO; GO:0072148; P:epithelial cell fate commitment; IMP:MGI.
DR GO; GO:0002068; P:glandular epithelial cell development; IMP:MGI.
DR GO; GO:0060576; P:intestinal epithelial cell development; IMP:MGI.
DR GO; GO:0060480; P:lung goblet cell differentiation; IDA:MGI.
DR GO; GO:0010454; P:negative regulation of cell fate commitment; IMP:MGI.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:0043065; P:positive regulation of apoptotic process; ISO:MGI.
DR GO; GO:0010455; P:positive regulation of cell fate commitment; IMP:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IDA:MGI.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.10.150.50; -; 1.
DR InterPro; IPR000418; Ets_dom.
DR InterPro; IPR046328; ETS_fam.
DR InterPro; IPR003118; Pointed_dom.
DR InterPro; IPR013761; SAM/pointed_sf.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR11849; PTHR11849; 1.
DR Pfam; PF00178; Ets; 1.
DR Pfam; PF02198; SAM_PNT; 1.
DR PRINTS; PR00454; ETSDOMAIN.
DR SMART; SM00413; ETS; 1.
DR SMART; SM00251; SAM_PNT; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF47769; SSF47769; 1.
DR PROSITE; PS00345; ETS_DOMAIN_1; 1.
DR PROSITE; PS00346; ETS_DOMAIN_2; 1.
DR PROSITE; PS50061; ETS_DOMAIN_3; 1.
DR PROSITE; PS51433; PNT; 1.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..325
FT /note="SAM pointed domain-containing Ets transcription
FT factor"
FT /id="PRO_0000223959"
FT DOMAIN 119..203
FT /note="PNT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00762"
FT DNA_BIND 239..322
FT /note="ETS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00237"
FT REGION 27..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 79..100
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 325 AA; 36356 MW; B55CF6CEC4501125 CRC64;
MGSASPGLSN VSPGCLLLFP DVAPRTGTEK AASGAMGPEK QEWSPSPPAT PEQGLSAFYL
SYFNMYPDDS SWVAKVPEAR AGEDHPEEPE QCPVIDSQAS GSTLDEHSLE QVQSMVVGEV
LKDIETACKL LNITADPGDW SPGNVQKWLL WTEHQYRLPP AGKAFQELGG KELCAMSEEQ
FRQRAPLGGD VLHAHLDIWK SAAWMKERTS PGTLHYCAST SEEGWTDGEV DSSCSGQPIH
LWQFLKELLL KPHSYGRFIR WLNKEKGIFK IEDSAQVARL WGVRKNRPAM NYDKLSRSIR
QYYKKGIIRK PDISQRLVYQ FVHPV
