ID   SPDE_NICSY              Reviewed;         314 AA.
AC   O48660;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Spermidine synthase;
DE            EC=2.5.1.16;
DE   AltName: Full=Putrescine aminopropyltransferase;
DE            Short=Aminopropyltransferase;
OS   Nicotiana sylvestris (Wood tobacco) (South American tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4096;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Leaf;
RX   PubMed=9517003; DOI=10.1093/oxfordjournals.pcp.a029291;
RA   Hashimoto T., Tamaki K., Suzuki K., Yamada Y.;
RT   "Molecular cloning of plant spermidine synthases.";
RL   Plant Cell Physiol. 39:73-79(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=putrescine + S-adenosyl 3-(methylsulfanyl)propylamine = H(+) +
CC         S-methyl-5'-thioadenosine + spermidine; Xref=Rhea:RHEA:12721,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17509, ChEBI:CHEBI:57443,
CC         ChEBI:CHEBI:57834, ChEBI:CHEBI:326268; EC=2.5.1.16;
CC   -!- PATHWAY: Amine and polyamine biosynthesis; spermidine biosynthesis;
CC       spermidine from putrescine: step 1/1.
CC   -!- TISSUE SPECIFICITY: Root, stem and leaf.
CC   -!- SIMILARITY: Belongs to the spermidine/spermine synthase family.
CC       {ECO:0000305}.
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DR   EMBL; AB006692; BAA24535.1; -; mRNA.
DR   PIR; T15045; T15045.
DR   RefSeq; NP_001289514.1; NM_001302585.1.
DR   AlphaFoldDB; O48660; -.
DR   SMR; O48660; -.
DR   STRING; 4096.XP_009777991.1; -.
DR   GeneID; 104227438; -.
DR   eggNOG; KOG1562; Eukaryota.
DR   UniPathway; UPA00248; UER00314.
DR   Proteomes; UP000189701; Unplaced.
DR   GO; GO:0004766; F:spermidine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008295; P:spermidine biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 2.30.140.10; -; 1.
DR   Gene3D; 3.40.50.150; -; 1.
DR   HAMAP; MF_00198; Spermidine_synth; 1.
DR   InterPro; IPR030374; PABS.
DR   InterPro; IPR030373; PABS_CS.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR001045; Spermi_synthase.
DR   InterPro; IPR030668; Spermi_synthase_euk.
DR   InterPro; IPR035246; Spermidine_synt_N.
DR   InterPro; IPR037163; Spermidine_synt_N_sf.
DR   PANTHER; PTHR11558; PTHR11558; 1.
DR   Pfam; PF17284; Spermine_synt_N; 1.
DR   PIRSF; PIRSF000502; Spermidine_synth; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   TIGRFAMs; TIGR00417; speE; 1.
DR   PROSITE; PS01330; PABS_1; 1.
DR   PROSITE; PS51006; PABS_2; 1.
PE   2: Evidence at transcript level;
KW   Polyamine biosynthesis; Reference proteome; Spermidine biosynthesis;
KW   Transferase.
FT   CHAIN           1..314
FT                   /note="Spermidine synthase"
FT                   /id="PRO_0000156456"
FT   DOMAIN          23..260
FT                   /note="PABS"
FT   ACT_SITE        179
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         54
FT                   /ligand="S-adenosyl 3-(methylsulfanyl)propylamine"
FT                   /ligand_id="ChEBI:CHEBI:57443"
FT                   /evidence="ECO:0000250"
FT   BINDING         84
FT                   /ligand="putrescine"
FT                   /ligand_id="ChEBI:CHEBI:326268"
FT                   /evidence="ECO:0000250"
FT   BINDING         85
FT                   /ligand="S-adenosyl 3-(methylsulfanyl)propylamine"
FT                   /ligand_id="ChEBI:CHEBI:57443"
FT                   /evidence="ECO:0000250"
FT   BINDING         109
FT                   /ligand="S-adenosyl 3-(methylsulfanyl)propylamine"
FT                   /ligand_id="ChEBI:CHEBI:57443"
FT                   /evidence="ECO:0000250"
FT   BINDING         129
FT                   /ligand="S-adenosyl 3-(methylsulfanyl)propylamine"
FT                   /ligand_id="ChEBI:CHEBI:57443"
FT                   /evidence="ECO:0000250"
FT   BINDING         160..161
FT                   /ligand="S-adenosyl 3-(methylsulfanyl)propylamine"
FT                   /ligand_id="ChEBI:CHEBI:57443"
FT                   /evidence="ECO:0000250"
FT   BINDING         179..182
FT                   /ligand="putrescine"
FT                   /ligand_id="ChEBI:CHEBI:326268"
FT                   /evidence="ECO:0000250"
FT   BINDING         179
FT                   /ligand="S-adenosyl 3-(methylsulfanyl)propylamine"
FT                   /ligand_id="ChEBI:CHEBI:57443"
FT                   /evidence="ECO:0000250"
FT   BINDING         248
FT                   /ligand="putrescine"
FT                   /ligand_id="ChEBI:CHEBI:326268"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   314 AA;  34512 MW;  6C7707D92F61E869 CRC64;
     MEAANHNNNG CTNESPYISS VLPGWFSEIS PLWPGEAHSL KVEKILFQGK SDYQNVMVFQ
     SSTYGKVLVL DGVIQLTERD ECAYQEMIAH LPLCSIPNPK KVLVIGGGDG GVLREVSRHS
     SVEQIDICEI DKMVVEVSKQ FFPDVAVGYE DPRVNLHIGD GVAFLKNVAA GTYDAVIVDS
     SDPIGPAQEL FEKPFFESIA RALRPGGVVS TQAESIWLHM HIIEEIVANC RQIFKGSVNY
     AWTTVPTYPS GMIGFMLCST EGPAVDFKNP INPIDDDASH NKTLGPMKFY NSELHKASFC
     LPSFAKRVIE SKGK