ID   SPDYA_RAT               Reviewed;         312 AA.
AC   Q8R496; Q68G34;
DT   02-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Speedy protein A;
DE   AltName: Full=GS4;
DE   AltName: Full=Rapid inducer of G2/M progression in oocytes A;
DE            Short=RINGO A;
DE   AltName: Full=Speedy-1;
DE            Short=Spy1;
GN   Name=Spdya {ECO:0000250|UniProtKB:Q5MJ70};
GN   Synonyms=Spdy1 {ECO:0000312|RGD:621730};
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1] {ECO:0000312|EMBL:AAM09568.1}
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Testis {ECO:0000312|EMBL:AAM09568.1};
RA   Zhao L., Jia M.;
RT   "Gene related to spermatogenesis.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Regulates the G1/S phase transition of the cell cycle by
CC       binding and activating CDK1 and CDK2. Contributes to CDK2 activation
CC       without promoting CDK2 phosphorylation, by inducing a conformation
CC       change of the CDK2 T-loop that obstructs the substrate-binding cleft
CC       prior to kinase activation. Interferes with CDKN1B-mediated inhibition
CC       of CDK2. Mediates cell survival during the DNA damage process through
CC       activation of CDK2. {ECO:0000250|UniProtKB:Q5IBH7,
CC       ECO:0000250|UniProtKB:Q5MJ70}.
CC   -!- SUBUNIT: Interacts with CDK1 (By similarity). Interacts with CDK2. May
CC       interact with CDKN1B/KIP1. Identified in a complex with CDK2 and
CC       CDKN1B/KIP1, where it interacts primarily with CDK2 (By similarity).
CC       {ECO:0000250|UniProtKB:Q5IBH7, ECO:0000250|UniProtKB:Q5MJ70}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q5MJ70}.
CC   -!- DOMAIN: The C-terminus is required for CDK2-activation, but not CDK2-
CC       binding. {ECO:0000250|UniProtKB:Q5IBH7}.
CC   -!- SIMILARITY: Belongs to the Speedy/Ringo family. {ECO:0000305}.
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DR   EMBL; AF492385; AAM09568.1; -; mRNA.
DR   EMBL; BC078729; AAH78729.1; -; mRNA.
DR   RefSeq; NP_620210.1; NM_138855.1.
DR   RefSeq; XP_006239830.1; XM_006239768.3.
DR   AlphaFoldDB; Q8R496; -.
DR   SMR; Q8R496; -.
DR   STRING; 10116.ENSRNOP00000006073; -.
DR   PaxDb; Q8R496; -.
DR   PRIDE; Q8R496; -.
DR   Ensembl; ENSRNOT00000006073; ENSRNOP00000006073; ENSRNOG00000004534.
DR   GeneID; 192209; -.
DR   KEGG; rno:192209; -.
DR   UCSC; RGD:621730; rat.
DR   CTD; 245711; -.
DR   RGD; 621730; Spdya.
DR   eggNOG; KOG3938; Eukaryota.
DR   GeneTree; ENSGT00940000154524; -.
DR   InParanoid; Q8R496; -.
DR   OMA; VCQTPPT; -.
DR   OrthoDB; 1480540at2759; -.
DR   PhylomeDB; Q8R496; -.
DR   TreeFam; TF329827; -.
DR   PRO; PR:Q8R496; -.
DR   Proteomes; UP000002494; Chromosome 6.
DR   Bgee; ENSRNOG00000004534; Expressed in testis and 12 other tissues.
DR   Genevisible; Q8R496; RN.
DR   GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0030295; F:protein kinase activator activity; ISO:RGD.
DR   GO; GO:0019901; F:protein kinase binding; ISS:UniProtKB.
DR   GO; GO:0006974; P:cellular response to DNA damage stimulus; ISO:RGD.
DR   GO; GO:0000082; P:G1/S transition of mitotic cell cycle; ISS:UniProtKB.
DR   GO; GO:0007140; P:male meiotic nuclear division; ISS:UniProtKB.
DR   GO; GO:0008284; P:positive regulation of cell population proliferation; ISO:RGD.
DR   GO; GO:0045737; P:positive regulation of cyclin-dependent protein serine/threonine kinase activity; ISS:UniProtKB.
DR   GO; GO:0045860; P:positive regulation of protein kinase activity; ISO:RGD.
DR   InterPro; IPR020984; Speedy.
DR   Pfam; PF11357; Spy1; 1.
PE   2: Evidence at transcript level;
KW   Cell cycle; Developmental protein; DNA damage; Nucleus; Phosphoprotein;
KW   Reference proteome.
FT   CHAIN           1..312
FT                   /note="Speedy protein A"
FT                   /id="PRO_0000234114"
FT   REGION          67..199
FT                   /note="Speedy/Ringo box; Required for CDK-binding"
FT                   /evidence="ECO:0000250|UniProtKB:Q5IBH7"
FT   MOD_RES         221
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q5IBH7"
FT   MOD_RES         223
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:Q5IBH7"
FT   CONFLICT        283
FT                   /note="Missing (in Ref. 2; AAM09568)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   312 AA;  36223 MW;  7ACAAB7EF1CAA81E CRC64;
     MRHNQMCCET PPTVTVHVKS GSNRSHQTRK PVSLKRPILK DSWQASEKNA HNNKPKRPRG
     PCLIIQRQEM TAFFKLFDDD LIQDFLWMDC CCKIADKYLL AMTFVYFKRA KFTISEHTRI
     NFFIALYLAN TVEEDEEEAK YEIFPWALGK NWRKLFPNFL KLRDQLWDRI DYRAIVSRRC
     CEEVMAIAPS HYIWQRERSV HHSGAARNYN RDEVHLPRGP SATPVDCSLC GKKGRYVRLG
     LSSSSSSSSD IVELTGKRSQ ELHNSLSMDM IGDPSQANTY SQVANDHQSK KENETNFVKK
     TKSMGWFAES EE