SPDYC_HUMAN
ID SPDYC_HUMAN Reviewed; 293 AA.
AC Q5MJ68; B2RP32; B7ZMH7;
DT 02-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Speedy protein C;
DE AltName: Full=Rapid inducer of G2/M progression in oocytes C;
DE Short=RINGO C;
DE Short=hSpy/Ringo C;
GN Name=SPDYC {ECO:0000312|HGNC:HGNC:32681};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAW30396.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH CDK1 AND CDK2, AND
RP TISSUE SPECIFICITY.
RC TISSUE=Testis {ECO:0000312|EMBL:AAW30396.1};
RX PubMed=15611625; DOI=10.4161/cc.4.1.1347;
RA Cheng A., Xiong W., Ferrell J.E. Jr., Solomon M.J.;
RT "Identification and comparative analysis of multiple mammalian Speedy/Ringo
RT proteins.";
RL Cell Cycle 4:155-165(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, INTERACTION WITH AURKB, AND SUBCELLULAR LOCATION.
RX PubMed=20605920; DOI=10.1242/jcs.059964;
RA Mouron S., de Carcer G., Seco E., Fernandez-Miranda G., Malumbres M.,
RA Nebreda A.R.;
RT "RINGO C is required to sustain the spindle-assembly checkpoint.";
RL J. Cell Sci. 123:2586-2595(2010).
CC -!- FUNCTION: Promotes progression through the cell cycle via binding and
CC activation of CDK1 and CDK2. Involved in the spindle-assembly
CC checkpoint. Required for recruitment of MAD2L1, BUBR1 and BUB1 to
CC kinetochores. Required for the correct localization of the active form
CC of Aurora B in prometaphase. {ECO:0000269|PubMed:15611625,
CC ECO:0000269|PubMed:20605920}.
CC -!- SUBUNIT: Interacts with CDK1 and CDK2. Interacts with AURKB.
CC {ECO:0000269|PubMed:15611625, ECO:0000269|PubMed:20605920}.
CC -!- INTERACTION:
CC Q5MJ68; P56211: ARPP19; NbExp=3; IntAct=EBI-12162209, EBI-5773880;
CC Q5MJ68; Q9Y324: FCF1; NbExp=3; IntAct=EBI-12162209, EBI-5455734;
CC Q5MJ68; Q9Y250: LZTS1; NbExp=3; IntAct=EBI-12162209, EBI-1216080;
CC Q5MJ68; P26367: PAX6; NbExp=3; IntAct=EBI-12162209, EBI-747278;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:20605920}.
CC Note=Colocalizes with tubulin gamma during interphase. During mitosis,
CC enriched at the spindle poles and the midbody during telophase.
CC -!- TISSUE SPECIFICITY: Expressed in a variety of tissues including bone
CC marrow, kidney, small intestine, liver, placenta and testis.
CC {ECO:0000269|PubMed:15611625}.
CC -!- DOMAIN: The C-terminus is required for CDK2-activation, but not CDK2-
CC binding. {ECO:0000250|UniProtKB:Q5IBH7}.
CC -!- SIMILARITY: Belongs to the Speedy/Ringo family. {ECO:0000305}.
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DR EMBL; AY820305; AAW30396.1; -; mRNA.
DR EMBL; BC137244; AAI37245.1; -; mRNA.
DR EMBL; BC137246; AAI37247.1; -; mRNA.
DR EMBL; BC144548; AAI44549.1; -; mRNA.
DR RefSeq; NP_001008778.1; NM_001008778.1.
DR AlphaFoldDB; Q5MJ68; -.
DR SMR; Q5MJ68; -.
DR BioGRID; 132439; 47.
DR IntAct; Q5MJ68; 4.
DR STRING; 9606.ENSP00000366390; -.
DR iPTMnet; Q5MJ68; -.
DR PhosphoSitePlus; Q5MJ68; -.
DR BioMuta; SPDYC; -.
DR DMDM; 74706914; -.
DR PaxDb; Q5MJ68; -.
DR PRIDE; Q5MJ68; -.
DR ProteomicsDB; 63584; -.
DR Antibodypedia; 50287; 29 antibodies from 11 providers.
DR DNASU; 387778; -.
DR Ensembl; ENST00000377185.2; ENSP00000366390.2; ENSG00000204710.2.
DR GeneID; 387778; -.
DR KEGG; hsa:387778; -.
DR MANE-Select; ENST00000377185.3; ENSP00000366390.3; NM_001008778.3; NP_001008778.2.
DR UCSC; uc010rnz.3; human.
DR CTD; 387778; -.
DR DisGeNET; 387778; -.
DR GeneCards; SPDYC; -.
DR HGNC; HGNC:32681; SPDYC.
DR HPA; ENSG00000204710; Group enriched (liver, testis).
DR MIM; 614030; gene.
DR neXtProt; NX_Q5MJ68; -.
DR OpenTargets; ENSG00000204710; -.
DR PharmGKB; PA144596271; -.
DR VEuPathDB; HostDB:ENSG00000204710; -.
DR eggNOG; ENOG502SPHM; Eukaryota.
DR GeneTree; ENSGT00940000154524; -.
DR HOGENOM; CLU_070353_2_1_1; -.
DR InParanoid; Q5MJ68; -.
DR OMA; MAKEPTH; -.
DR OrthoDB; 1480540at2759; -.
DR PhylomeDB; Q5MJ68; -.
DR TreeFam; TF329827; -.
DR PathwayCommons; Q5MJ68; -.
DR SignaLink; Q5MJ68; -.
DR BioGRID-ORCS; 387778; 21 hits in 1080 CRISPR screens.
DR GenomeRNAi; 387778; -.
DR Pharos; Q5MJ68; Tdark.
DR PRO; PR:Q5MJ68; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; Q5MJ68; protein.
DR Bgee; ENSG00000204710; Expressed in right lobe of liver and 35 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0019901; F:protein kinase binding; IPI:UniProtKB.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0045737; P:positive regulation of cyclin-dependent protein serine/threonine kinase activity; IEA:InterPro.
DR InterPro; IPR020984; Speedy.
DR Pfam; PF11357; Spy1; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cytoplasm; Reference proteome.
FT CHAIN 1..293
FT /note="Speedy protein C"
FT /id="PRO_0000234116"
FT REGION 56..188
FT /note="Speedy/Ringo box; Required for CDK-binding"
FT /evidence="ECO:0000250|UniProtKB:Q5IBH7"
SQ SEQUENCE 293 AA; 33166 MW; DA3031A3CBD3A1DC CRC64;
MLWAIPELGS PCPISISYEM SDSQDPTTSP VVTTQVELGG CSRQGGGNGF LRFRQHQEVQ
AFLSLLEDSF VQEFLSKDPC FQISDKYLLA MVLVYFQRAH LKLSEYTHSS LFLALYLAND
MEEDLEGPKC EIFPWALGKD WCLRVGKFLH QRDKLWARMG FRAVVSRQCC EEVMAKEPFH
WAWTRDRRPH HGGVQRVCPQ VPVRLPRGPG LSPPHCSPCG LPQHCSSHLL KPVSSKCPSL
TSECHRPPSQ NYLSRVKNAW GGDFLIVLPP QMQLEPGTYS LRIFPKPPAR PGH
