SPE1A_STRPU
ID SPE1A_STRPU Reviewed; 152 AA.
AC P04109;
DT 01-NOV-1986, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 3.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Calcium-binding protein SPEC 1A;
GN Name=SPEC1;
OS Strongylocentrotus purpuratus (Purple sea urchin).
OC Eukaryota; Metazoa; Echinodermata; Eleutherozoa; Echinozoa; Echinoidea;
OC Euechinoidea; Echinacea; Camarodonta; Echinidea; Strongylocentrotidae;
OC Strongylocentrotus.
OX NCBI_TaxID=7668;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2935638; DOI=10.1016/0022-2836(85)90101-9;
RA Hardin S.H., Carpenter C.D., Hardin P.E., Bruskin A.M., Klein W.H.;
RT "Structure of the Spec1 gene encoding a major calcium-binding protein in
RT the embryonic ectoderm of the sea urchin, Strongylocentrotus purpuratus.";
RL J. Mol. Biol. 186:243-255(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Embryo;
RX PubMed=3116272; DOI=10.1007/bf02101754;
RA Hardin P.E., Klein W.H.;
RT "Unusual sequence conservation in the 5' and 3' untranslated regions of the
RT sea urchin spec mRNAs.";
RL J. Mol. Evol. 25:126-133(1987).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 67-152.
RX PubMed=6697391; DOI=10.1016/0092-8674(84)90346-5;
RA Carpenter C.D., Bruskin A.M., Hardin P.E., Keast M.J., Anstrom J.A.,
RA Tyner A.L., Brandhorst B.P., Klein W.H.;
RT "Novel proteins belonging to the troponin C superfamily are encoded by a
RT set of mRNAs in sea urchin embryos.";
RL Cell 36:663-671(1984).
CC -!- FUNCTION: Calcium-binding protein involved in larval development and
CC metamorphosis. Likely to function as calcium buffers mediating the
CC transport of calcium from the sea water to the blastocoel where calcium
CC is required for skeleton formation.
CC -!- TISSUE SPECIFICITY: Found in cell lineages giving rise to the aboral
CC ectoderm, a squamous epithelium covering the surface of the late stage
CC embryo and larva.
CC -!- DEVELOPMENTAL STAGE: Accumulate in embryos and larvae, but not in
CC adults.
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DR EMBL; X03287; CAA27036.1; -; Genomic_DNA.
DR EMBL; X03288; CAA27036.1; JOINED; Genomic_DNA.
DR EMBL; X03289; CAA27036.1; JOINED; Genomic_DNA.
DR EMBL; X03290; CAA27036.1; JOINED; Genomic_DNA.
DR EMBL; X03291; CAA27036.1; JOINED; Genomic_DNA.
DR EMBL; X03292; CAA27036.1; JOINED; Genomic_DNA.
DR EMBL; M32446; AAA30073.1; -; mRNA.
DR EMBL; K01649; AAA30076.1; -; mRNA.
DR PIR; S03249; MCUR1C.
DR RefSeq; NP_999768.1; NM_214603.1.
DR AlphaFoldDB; P04109; -.
DR SMR; P04109; -.
DR STRING; 7668.SPU_007452-tr; -.
DR EnsemblMetazoa; NM_214603; NP_999768; GeneID_373443.
DR GeneID; 373443; -.
DR KEGG; spu:373443; -.
DR CTD; 373443; -.
DR eggNOG; KOG0027; Eukaryota.
DR HOGENOM; CLU_061288_2_1_1; -.
DR InParanoid; P04109; -.
DR OrthoDB; 618058at33208; -.
DR PhylomeDB; P04109; -.
DR Proteomes; UP000007110; Unassembled WGS sequence.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR CDD; cd00051; EFh; 2.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13499; EF-hand_7; 2.
DR SMART; SM00054; EFh; 4.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 4.
DR PROSITE; PS50222; EF_HAND_2; 4.
PE 2: Evidence at transcript level;
KW Calcium; Metal-binding; Reference proteome; Repeat.
FT CHAIN 1..152
FT /note="Calcium-binding protein SPEC 1A"
FT /id="PRO_0000073638"
FT DOMAIN 10..45
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 46..81
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 84..119
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 120..152
FT /note="EF-hand 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT REGION 95..121
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 23
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 25
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 27
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 29
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 34
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 59
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 61
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 63
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 65
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 70
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 97
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 99
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 101
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 103
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 108
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 133
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 135
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 137
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 139
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 144
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT CONFLICT 132
FT /note="A -> P (in Ref. 2; AAA30073)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 152 AA; 17466 MW; B7C68B9A68F261B2 CRC64;
MAAQLLFTDE EVTEFKRRFK NKDTDKSKSI TAEELGEFFK STGKSYTDKQ IDKMISDVDT
DESGTIDFSE MLMGIAEQMV KWTWKEEHYT KAFDDMDKDG NGSLSPQELR EALSASKPPM
KRKKIKAIIQ KADANKDGKI DREEFMKLIK SC
