ID   SPE26_CAEEL             Reviewed;         570 AA.
AC   Q10579;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 139.
DE   RecName: Full=Spermatocyte protein spe-26;
DE   AltName: Full=Defective spermatogenesis protein 26;
GN   Name=spe-26; ORFNames=R10H10.2;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, AND
RP   MUTAGENESIS OF SER-360 AND GLY-446.
RC   STRAIN=Bristol N2;
RX   PubMed=7744249; DOI=10.1101/gad.9.9.1074;
RA   Varkey J.P., Muhlrad P.J., Minniti A.N., Do B., Ward S.;
RT   "The Caenorhabditis elegans spe-26 gene is necessary to form spermatids and
RT   encodes a protein similar to the actin-associated proteins kelch and
RT   scruin.";
RL   Genes Dev. 9:1074-1086(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
CC   -!- FUNCTION: May play a role in the spermatocyte cytoskeleton, possibly
CC       interacting with actin. {ECO:0000269|PubMed:7744249}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
CC   -!- TISSUE SPECIFICITY: Testis, in both spermatogonial cells and
CC       spermatocytes. {ECO:0000269|PubMed:7744249}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U20142; AAB60235.1; -; Genomic_DNA.
DR   EMBL; Z70686; CAA94610.1; -; Genomic_DNA.
DR   PIR; T24152; T24152.
DR   RefSeq; NP_501919.1; NM_069518.3.
DR   AlphaFoldDB; Q10579; -.
DR   SMR; Q10579; -.
DR   BioGRID; 43031; 2.
DR   DIP; DIP-24461N; -.
DR   STRING; 6239.R10H10.2; -.
DR   PaxDb; Q10579; -.
DR   EnsemblMetazoa; R10H10.2.1; R10H10.2.1; WBGene00004972.
DR   GeneID; 177929; -.
DR   KEGG; cel:CELE_R10H10.2; -.
DR   UCSC; R10H10.2; c. elegans.
DR   CTD; 177929; -.
DR   WormBase; R10H10.2; CE06293; WBGene00004972; spe-26.
DR   eggNOG; KOG1072; Eukaryota.
DR   GeneTree; ENSGT00940000163715; -.
DR   HOGENOM; CLU_471929_0_0_1; -.
DR   InParanoid; Q10579; -.
DR   OMA; RIEWFDP; -.
DR   OrthoDB; 569226at2759; -.
DR   PhylomeDB; Q10579; -.
DR   PRO; PR:Q10579; -.
DR   Proteomes; UP000001940; Chromosome IV.
DR   Bgee; WBGene00004972; Expressed in germ cell and 6 other tissues.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR   GO; GO:0003779; F:actin binding; IEA:UniProtKB-KW.
DR   Gene3D; 2.120.10.80; -; 2.
DR   InterPro; IPR011705; BACK.
DR   InterPro; IPR015915; Kelch-typ_b-propeller.
DR   InterPro; IPR006652; Kelch_1.
DR   Pfam; PF07707; BACK; 1.
DR   Pfam; PF01344; Kelch_1; 4.
DR   SMART; SM00875; BACK; 1.
DR   SMART; SM00612; Kelch; 5.
DR   SUPFAM; SSF117281; SSF117281; 1.
PE   1: Evidence at protein level;
KW   Actin-binding; Cytoplasm; Cytoskeleton; Kelch repeat; Reference proteome;
KW   Repeat.
FT   CHAIN           1..570
FT                   /note="Spermatocyte protein spe-26"
FT                   /id="PRO_0000119145"
FT   REPEAT          244..291
FT                   /note="Kelch 1"
FT   REPEAT          293..338
FT                   /note="Kelch 2"
FT   REPEAT          341..393
FT                   /note="Kelch 3"
FT   REPEAT          395..440
FT                   /note="Kelch 4"
FT   REPEAT          442..487
FT                   /note="Kelch 5"
FT   REPEAT          489..535
FT                   /note="Kelch 6"
FT   MUTAGEN         360
FT                   /note="S->N: In IT112ts; causes sterility at 25 degrees
FT                   Celsius."
FT                   /evidence="ECO:0000269|PubMed:7744249"
FT   MUTAGEN         446
FT                   /note="G->E: In HC140ts; causes sterility at 25 degrees
FT                   Celsius."
FT                   /evidence="ECO:0000269|PubMed:7744249"
SQ   SEQUENCE   570 AA;  65552 MW;  B2092B2B79380DB7 CRC64;
     MRFCNSKLTS IDNRCDVAII SKDEERFEVK LVESEIAREL NRLGALSPRS MQKNILVPFT
     TQTVKYLLGL EKVSDSTDEM GWFGIEKSLE EIAGFGQHCF PKILEMKNTI CDHILSTLSD
     VNCFLLHKKF REFDCQNHAH KTLEYILYNL ARMVVVDKRV DVEFYRLPVE EVKHLLSSEE
     VNVEQETQII EVINQWIAAD FENRDKFRPM LMSTVRFLAL DQQIAKSLSQ FHPSLKPARR
     TRDVLIIIGG WLHRQACDRI EWFDPENNCW KVSQQKLPTT LAYHGSAIVD GILYLFGGST
     GQRTRCETWK LSTETWQWDR CNNMMEPRNY ISNSSVVYDG RIYVFGGQNF REITRTAVRS
     RTGEVFDPKT NKWTETASLS DMRSDCAAEV FENQIYVSGG FNGDMILASV EVYNPIGNVF
     SRTVDLPYPI TGHCLLNHGN QLLIVGGFDG AERQNKIWMW HRTGEWQQRP EKLIYGRSTS
     AACSYKGWLF SVAGYTEKVE ATCEILLPEP NASRFSFIPD VPRAKSALNV LVAPNWRNFL
     ERRGTINEQS MEMDDDYEDD AGASYMSINN