ID   SPE2C_STRPU             Reviewed;         151 AA.
AC   P04111;
DT   01-NOV-1986, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 2.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=Calcium-binding protein SPEC 2C;
GN   Name=SPEC2C;
OS   Strongylocentrotus purpuratus (Purple sea urchin).
OC   Eukaryota; Metazoa; Echinodermata; Eleutherozoa; Echinozoa; Echinoidea;
OC   Euechinoidea; Echinacea; Camarodonta; Echinidea; Strongylocentrotidae;
OC   Strongylocentrotus.
OX   NCBI_TaxID=7668;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6697391; DOI=10.1016/0092-8674(84)90346-5;
RA   Carpenter C.D., Bruskin A.M., Hardin P.E., Keast M.J., Anstrom J.A.,
RA   Tyner A.L., Brandhorst B.P., Klein W.H.;
RT   "Novel proteins belonging to the troponin C superfamily are encoded by a
RT   set of mRNAs in sea urchin embryos.";
RL   Cell 36:663-671(1984).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3172223; DOI=10.1016/0022-2836(88)90275-6;
RA   Hardin P.E., Angerer L.M., Hardin S.H., Angerer R.C., Klein W.H.;
RT   "Spec2 genes of Strongylocentrotus purpuratus. Structure and differential
RT   expression in embryonic aboral ectoderm cells.";
RL   J. Mol. Biol. 202:417-431(1988).
CC   -!- FUNCTION: Calcium-binding protein involved in larval development and
CC       metamorphosis. Likely to function as calcium buffers mediating the
CC       transport of calcium from the sea water to the blastocoel where calcium
CC       is required for skeleton formation.
CC   -!- TISSUE SPECIFICITY: Found in cell lineages giving rise to the aboral
CC       ectoderm, a squamous epithelium covering the surface of the late stage
CC       embryo and larva.
CC   -!- DEVELOPMENTAL STAGE: Accumulate in embryos and larvae, but not in
CC       adults.
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DR   EMBL; X12772; CAA31260.2; -; Genomic_DNA.
DR   EMBL; X14534; CAA31260.2; JOINED; Genomic_DNA.
DR   EMBL; X14535; CAA31260.2; JOINED; Genomic_DNA.
DR   EMBL; X14536; CAA31260.2; JOINED; Genomic_DNA.
DR   EMBL; X14537; CAA31260.2; JOINED; Genomic_DNA.
DR   EMBL; X14538; CAA31260.2; JOINED; Genomic_DNA.
DR   PIR; S01771; MCUR2C.
DR   RefSeq; NP_999769.1; NM_214604.1.
DR   AlphaFoldDB; P04111; -.
DR   SMR; P04111; -.
DR   STRING; 7668.SPU_007449-tr; -.
DR   EnsemblMetazoa; NM_214604; NP_999769; GeneID_373447.
DR   GeneID; 373447; -.
DR   KEGG; spu:373447; -.
DR   CTD; 373447; -.
DR   eggNOG; KOG0027; Eukaryota.
DR   HOGENOM; CLU_2609113_0_0_1; -.
DR   InParanoid; P04111; -.
DR   OMA; CRCEFTE; -.
DR   OrthoDB; 979297at2759; -.
DR   PhylomeDB; P04111; -.
DR   Proteomes; UP000007110; Unassembled WGS sequence.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   CDD; cd00051; EFh; 2.
DR   InterPro; IPR011992; EF-hand-dom_pair.
DR   InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR   InterPro; IPR002048; EF_hand_dom.
DR   Pfam; PF13499; EF-hand_7; 2.
DR   SMART; SM00054; EFh; 4.
DR   SUPFAM; SSF47473; SSF47473; 1.
DR   PROSITE; PS00018; EF_HAND_1; 2.
DR   PROSITE; PS50222; EF_HAND_2; 4.
PE   2: Evidence at transcript level;
KW   Calcium; Metal-binding; Reference proteome; Repeat.
FT   CHAIN           1..151
FT                   /note="Calcium-binding protein SPEC 2C"
FT                   /id="PRO_0000073640"
FT   DOMAIN          10..45
FT                   /note="EF-hand 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          46..78
FT                   /note="EF-hand 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          81..116
FT                   /note="EF-hand 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          118..151
FT                   /note="EF-hand 4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         23
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         25
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         27
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         29
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         34
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         59
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000305"
FT   BINDING         61
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000305"
FT   BINDING         63
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000305"
FT   BINDING         70
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000305"
FT   BINDING         94
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         96
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         98
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         100
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         105
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         131
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="4"
FT                   /evidence="ECO:0000305"
FT   BINDING         135
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="4"
FT                   /evidence="ECO:0000305"
FT   BINDING         137
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="4"
FT                   /evidence="ECO:0000305"
FT   BINDING         142
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="4"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        45
FT                   /note="E -> D (in Ref. 1)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        77
FT                   /note="E -> L (in Ref. 1)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        137
FT                   /note="E -> K (in Ref. 1)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        140..141
FT                   /note="RA -> VQ (in Ref. 1)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        148
FT                   /note="Q -> E (in Ref. 1)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   151 AA;  16991 MW;  0ACAAC12A3E3E1B9 CRC64;
     MAVQLFFTEE QRKVFKSSFK SIDADGDGKI TPEELKAAFK SIEIELTQEK IDEMMSMVDK
     DGSRPVDFSE ILMKKAEQMR GKGAQYFKAF DALDTDKSGS LSPEELRTAL SACTDPPMTK
     EEIDAIIKKA DGNNDGEIRR AEFVRMIQSS Y