SPE2D_STRPU
ID SPE2D_STRPU Reviewed; 141 AA.
AC P15844;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1990, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Calcium-binding protein SPEC 2D;
GN Name=SPEC2D;
OS Strongylocentrotus purpuratus (Purple sea urchin).
OC Eukaryota; Metazoa; Echinodermata; Eleutherozoa; Echinozoa; Echinoidea;
OC Euechinoidea; Echinacea; Camarodonta; Echinidea; Strongylocentrotidae;
OC Strongylocentrotus.
OX NCBI_TaxID=7668;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Embryo;
RX PubMed=3116272; DOI=10.1007/bf02101754;
RA Hardin P.E., Klein W.H.;
RT "Unusual sequence conservation in the 5' and 3' untranslated regions of the
RT sea urchin spec mRNAs.";
RL J. Mol. Evol. 25:126-133(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3172223; DOI=10.1016/0022-2836(88)90275-6;
RA Hardin P.E., Angerer L.M., Hardin S.H., Angerer R.C., Klein W.H.;
RT "Spec2 genes of Strongylocentrotus purpuratus. Structure and differential
RT expression in embryonic aboral ectoderm cells.";
RL J. Mol. Biol. 202:417-431(1988).
CC -!- FUNCTION: Calcium-binding protein involved in larval development and
CC metamorphosis. Likely to function as calcium buffers mediating the
CC transport of calcium from the sea water to the blastocoel where calcium
CC is required for skeleton formation.
CC -!- TISSUE SPECIFICITY: Found in cell lineages giving rise to the aboral
CC ectoderm, a squamous epithelium covering the surface of the late stage
CC embryo and larva.
CC -!- DEVELOPMENTAL STAGE: Accumulate in embryos and larvae, but not in
CC adults.
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DR EMBL; M32447; AAA30074.1; -; mRNA.
DR EMBL; X12773; CAA31261.2; -; Genomic_DNA.
DR EMBL; X14539; CAA31261.2; JOINED; Genomic_DNA.
DR EMBL; X14540; CAA31261.2; JOINED; Genomic_DNA.
DR EMBL; X14541; CAA31261.2; JOINED; Genomic_DNA.
DR EMBL; X14542; CAA31261.2; JOINED; Genomic_DNA.
DR EMBL; X14543; CAA31261.2; JOINED; Genomic_DNA.
DR PIR; S01772; S01772.
DR AlphaFoldDB; P15844; -.
DR SMR; P15844; -.
DR STRING; 7668.SPU_003175-tr; -.
DR eggNOG; KOG0027; Eukaryota.
DR HOGENOM; CLU_438277_0_0_1; -.
DR InParanoid; P15844; -.
DR Proteomes; UP000007110; Unassembled WGS sequence.
DR GO; GO:0005509; F:calcium ion binding; IBA:GO_Central.
DR GO; GO:0030234; F:enzyme regulator activity; IBA:GO_Central.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR002048; EF_hand_dom.
DR SMART; SM00054; EFh; 3.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS50222; EF_HAND_2; 3.
PE 2: Evidence at transcript level;
KW Calcium; Metal-binding; Reference proteome; Repeat.
FT CHAIN 1..141
FT /note="Calcium-binding protein SPEC 2D"
FT /id="PRO_0000073642"
FT DOMAIN 10..42
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 43..72
FT /note="EF-hand 2"
FT /evidence="ECO:0000305"
FT DOMAIN 73..107
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 108..141
FT /note="EF-hand 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 23
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000305"
FT BINDING 25
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000305"
FT BINDING 27
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000305"
FT BINDING 29
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000305"
FT BINDING 84
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000305"
FT BINDING 86
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000305"
FT BINDING 90
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000305"
FT BINDING 95
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000305"
FT BINDING 121
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000305"
FT BINDING 125
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000305"
FT BINDING 127
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000305"
FT BINDING 132
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000305"
FT CONFLICT 26
FT /note="N -> S (in Ref. 1; AAA30074)"
FT /evidence="ECO:0000305"
FT CONFLICT 57
FT /note="V -> D (in Ref. 1; AAA30074)"
FT /evidence="ECO:0000305"
FT CONFLICT 66
FT /note="Q -> E (in Ref. 1; AAA30074)"
FT /evidence="ECO:0000305"
FT CONFLICT 101
FT /note="M -> V (in Ref. 1; AAA30074)"
FT /evidence="ECO:0000305"
FT CONFLICT 123
FT /note="P -> T (in Ref. 1; AAA30074)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 141 AA; 16268 MW; 43DF140420EA9ACA CRC64;
MAANLLFSED QIKEYKTKFD AFDRNNDGNF PTMFLGNAMK SVGHVLTAAE LENSRRVRKG
TTTFPQFLAM ILDKKCRKVF KAMDKDDKDK LLSADEVRQA MLSFDRQITE DKIKEMIEKA
DFPNDGKCSL EEFVKMVMNF C
