SPE44_CAEEL
ID SPE44_CAEEL Reviewed; 424 AA.
AC Q18171;
DT 07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Transcription regulator spe-44 {ECO:0000305};
DE AltName: Full=Spermatogenesis defective 44 {ECO:0000303|PubMed:22570621, ECO:0000312|WormBase:C25G4.4};
GN Name=spe-44 {ECO:0000312|WormBase:C25G4.4};
GN ORFNames=C25G4.4 {ECO:0000312|WormBase:C25G4.4};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=22570621; DOI=10.1371/journal.pgen.1002678;
RA Kulkarni M., Shakes D.C., Guevel K., Smith H.E.;
RT "SPE-44 implements sperm cell fate.";
RL PLoS Genet. 8:e1002678-e1002678(2012).
CC -!- FUNCTION: Transcription factor which controls spermatogenesis and sperm
CC cell fate by regulation of sperm gene expression.
CC {ECO:0000269|PubMed:22570621}.
CC -!- INTERACTION:
CC Q18171; Q9U9Y8: lit-1; NbExp=3; IntAct=EBI-320157, EBI-318513;
CC -!- SUBCELLULAR LOCATION: Chromosome {ECO:0000269|PubMed:22570621}. Nucleus
CC {ECO:0000255|PROSITE-ProRule:PRU00185, ECO:0000269|PubMed:22570621}.
CC Note=Localized along the length of most but not all of the autosomal
CC pachytene chromosomes; not detected on X chromosome.
CC {ECO:0000269|PubMed:22570621}.
CC -!- DEVELOPMENTAL STAGE: Expressed in larval stage L3 male and
CC hermaphrodite germlines, coinciding with sperm fate specification (at
CC protein level) (PubMed:22570621). Expression persists throughout
CC adulthood in males, but is absent in adult hermaphrodites that are
CC undergoing oogenesis (at protein level) (PubMed:22570621).
CC {ECO:0000269|PubMed:22570621}.
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DR EMBL; BX284604; CAA94573.1; -; Genomic_DNA.
DR PIR; T19470; T19470.
DR RefSeq; NP_502379.1; NM_069978.4.
DR AlphaFoldDB; Q18171; -.
DR SMR; Q18171; -.
DR DIP; DIP-27457N; -.
DR IntAct; Q18171; 40.
DR STRING; 6239.C25G4.4; -.
DR PaxDb; Q18171; -.
DR EnsemblMetazoa; C25G4.4.1; C25G4.4.1; WBGene00007732.
DR GeneID; 182915; -.
DR KEGG; cel:CELE_C25G4.4; -.
DR UCSC; C25G4.4; c. elegans.
DR CTD; 182915; -.
DR WormBase; C25G4.4; CE05305; WBGene00007732; spe-44.
DR eggNOG; KOG4333; Eukaryota.
DR GeneTree; ENSGT00410000025596; -.
DR HOGENOM; CLU_584263_0_0_1; -.
DR InParanoid; Q18171; -.
DR OMA; DWKASIR; -.
DR OrthoDB; 692814at2759; -.
DR PhylomeDB; Q18171; -.
DR SignaLink; Q18171; -.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00007732; Expressed in germ line (C elegans) and 5 other tissues.
DR GO; GO:0030849; C:autosome; IDA:WormBase.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:WormBase.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:WormBase.
DR GO; GO:0007283; P:spermatogenesis; IMP:WormBase.
DR Gene3D; 3.10.390.10; -; 1.
DR InterPro; IPR024830; GMEB1/2.
DR InterPro; IPR010919; SAND-like_dom_sf.
DR InterPro; IPR000770; SAND_dom.
DR PANTHER; PTHR10417; PTHR10417; 1.
DR Pfam; PF01342; SAND; 1.
DR SMART; SM00258; SAND; 1.
DR SUPFAM; SSF63763; SSF63763; 1.
DR PROSITE; PS50864; SAND; 1.
PE 1: Evidence at protein level;
KW Chromosome; DNA-binding; Metal-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation; Zinc.
FT CHAIN 1..424
FT /note="Transcription regulator spe-44"
FT /id="PRO_0000452250"
FT DOMAIN 65..150
FT /note="SAND"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00185"
FT REGION 178..228
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 371..394
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 178..213
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 371..389
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 424 AA; 47729 MW; F4DA739A3CF3D3C0 CRC64;
MFGGDVSAAS VLPSHPISGS CDGFQAEGSD HHHSTMTPFQ ESQRNVYQIL YGHPANDDFP
RPITPLQITI PEGDASPTVP VSCGVVNGKM HLNLFMCPGI HQPCIEVGND LLSPKQFTIR
GDKERQKDWK ASIRVGRSSL RTHMEAMTID FYEHMNRCSG KCQSRNYVNA PSEEVLQARK
SKRTSEAGQL KYEIENEMAG KEADNDDNRK SAKKARGRPR GSVNKPRQMV KMEPQDDRFF
EEFFIDAPPL QSMSSNEEPT SSNKESNECT PFNDILNCLQ NDPMNFWSQM QQTGVIGHFC
DDIIVSAINL KQSVMDNPVT PTTANMLTRT AFALGIPPVV VHRVQSIERN AYQQRKHDEM
FNDIQSTLAE EHSVKYQPRT SSSSQESLHT AREFTEEKVE ELIDVCKYDD YPESECLPGP
SHIQ
