SPEF2_RAT
ID SPEF2_RAT Reviewed; 1801 AA.
AC Q9R095; Q4V8M1;
DT 21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT 21-AUG-2007, sequence version 2.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Sperm flagellar protein 2;
DE AltName: Full=Protein KPL2;
GN Name=Spef2; Synonyms=Kpl2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=10100999; DOI=10.1165/ajrcmb.20.4.3496;
RA Ostrowski L.E., Andrews K., Potdar P., Matsuura H., Jetten A.,
RA Nettesheim P.;
RT "Cloning and characterization of KPL2, a novel gene induced during
RT ciliogenesis of tracheal epithelial cells.";
RL Am. J. Respir. Cell Mol. Biol. 20:675-683(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RX PubMed=15057822; DOI=10.1038/nature02426;
RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA Mockrin S., Collins F.S.;
RT "Genome sequence of the Brown Norway rat yields insights into mammalian
RT evolution.";
RL Nature 428:493-521(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Required for correct axoneme development in spermatozoa.
CC Important for normal development of the manchette and sperm head
CC morphology. Essential for male fertility. Plays a role in localization
CC of the intraflagellar transport protein IFT20 to the manchette,
CC suggesting function as an adapter for dynein-mediated protein transport
CC during spermatogenesis. Also plays a role in bone growth where it seems
CC to be required for normal osteoblast differentiation.
CC {ECO:0000250|UniProtKB:Q8C9J3}.
CC -!- SUBUNIT: Interacts (via C-terminus) with IFT20. Interacts with DYNC1I2.
CC {ECO:0000250|UniProtKB:Q8C9J3}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum
CC {ECO:0000250|UniProtKB:Q9C093}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q8C9J3}. Golgi apparatus
CC {ECO:0000250|UniProtKB:Q8C9J3}. Note=Shows dynamic localization in
CC developing spermatozoa. Localizes to the manchette in step 10-12
CC elongating spermatids. Detected in the basal body and neck area of step
CC 13-14 spermatids. Localizes to the midpiece of the sperm tail in step
CC 15-16 spermatids. During the epididymal transport of spermatozoa,
CC expression in the sperm tail reduces and becomes concentrated at the
CC distal part of the midpiece. Detected in the Golgi apparatus of late
CC spermatocytes and round spermatids. Detected in the cytoplasm of
CC Sertoli cells. {ECO:0000250|UniProtKB:Q8C9J3}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9R095-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9R095-2; Sequence=VSP_027530;
CC Name=3;
CC IsoId=Q9R095-3; Sequence=VSP_027531, VSP_027532;
CC -!- TISSUE SPECIFICITY: Predominantly expressed in ciliated tissues such as
CC lung, trachea, testis, brain, and at lower levels in kidney and spleen.
CC {ECO:0000269|PubMed:10100999}.
CC -!- DEVELOPMENTAL STAGE: Expressed during the process of ciliated cell
CC differentiation. In seminiferous tubules of the testis, its expression
CC is stage-specific and is highest in spermatocytes and round spermatids.
CC {ECO:0000269|PubMed:10100999}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF102129; AAD56310.1; -; mRNA.
DR EMBL; AABR03012106; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC097318; AAH97318.1; -; mRNA.
DR RefSeq; NP_072142.1; NM_022620.1. [Q9R095-2]
DR RefSeq; XP_017446581.1; XM_017591092.1. [Q9R095-3]
DR AlphaFoldDB; Q9R095; -.
DR STRING; 10116.ENSRNOP00000056749; -.
DR jPOST; Q9R095; -.
DR PaxDb; Q9R095; -.
DR PRIDE; Q9R095; -.
DR GeneID; 64555; -.
DR KEGG; rno:64555; -.
DR CTD; 79925; -.
DR RGD; 620450; Spef2.
DR eggNOG; ENOG502QR7Y; Eukaryota.
DR InParanoid; Q9R095; -.
DR OrthoDB; 81320at2759; -.
DR PhylomeDB; Q9R095; -.
DR PRO; PR:Q9R095; -.
DR Proteomes; UP000002494; Chromosome 2.
DR Bgee; ENSRNOG00000058275; Expressed in testis and 4 other tissues.
DR ExpressionAtlas; Q9R095; baseline.
DR GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR GO; GO:0005576; C:extracellular region; IEA:GOC.
DR GO; GO:0005794; C:Golgi apparatus; ISO:RGD.
DR GO; GO:0002177; C:manchette; ISO:RGD.
DR GO; GO:0036126; C:sperm flagellum; ISS:UniProtKB.
DR GO; GO:0097225; C:sperm midpiece; ISO:RGD.
DR GO; GO:0048854; P:brain morphogenesis; ISO:RGD.
DR GO; GO:0090660; P:cerebrospinal fluid circulation; ISO:RGD.
DR GO; GO:0003351; P:epithelial cilium movement involved in extracellular fluid movement; ISO:RGD.
DR GO; GO:0044458; P:motile cilium assembly; ISO:RGD.
DR GO; GO:0120197; P:mucociliary clearance; ISO:RGD.
DR GO; GO:0060541; P:respiratory system development; ISO:RGD.
DR GO; GO:0048705; P:skeletal system morphogenesis; ISO:RGD.
DR GO; GO:0007288; P:sperm axoneme assembly; ISS:UniProtKB.
DR GO; GO:0007283; P:spermatogenesis; ISO:RGD.
DR Gene3D; 1.10.418.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR001715; CH-domain.
DR InterPro; IPR010441; CH_2.
DR InterPro; IPR036872; CH_dom_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF06294; CH_2; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS50021; CH; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell projection; Cilium; Coiled coil; Cytoplasm;
KW Differentiation; Flagellum; Golgi apparatus; Reference proteome;
KW Spermatogenesis.
FT CHAIN 1..1801
FT /note="Sperm flagellar protein 2"
FT /id="PRO_0000299032"
FT DOMAIN 1..105
FT /note="Calponin-homology (CH)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00044"
FT REGION 632..659
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 883..949
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1233..1304
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1305..1657
FT /note="Interaction with IFT20"
FT /evidence="ECO:0000250|UniProtKB:Q9C093"
FT REGION 1651..1695
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1781..1801
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 176..260
FT /evidence="ECO:0000255"
FT COILED 321..395
FT /evidence="ECO:0000255"
FT COILED 722..748
FT /evidence="ECO:0000255"
FT COILED 869..895
FT /evidence="ECO:0000255"
FT COILED 1051..1077
FT /evidence="ECO:0000255"
FT COILED 1252..1286
FT /evidence="ECO:0000255"
FT COILED 1665..1695
FT /evidence="ECO:0000255"
FT COMPBIAS 1233..1300
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1665..1694
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1784..1801
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 139..195
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:10100999"
FT /id="VSP_027530"
FT VAR_SEQ 509..514
FT /note="NMTGEW -> VQTNME (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_027531"
FT VAR_SEQ 515..1801
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_027532"
SQ SEQUENCE 1801 AA; 208159 MW; 38DC458176781AAE CRC64;
MSEILCQWLN QELKVSRTVS PKSFAKTFSN GYLIGEVLFK FELQSDFSEF SESRVSTAKL
NNFSRLQPTL HLLGVQFDQN VAQSIITEKP GAATKLLYQL YIALQKKKKT GLTGLEIQTM
QPQTNQRLQA LKSEAFRERL KNLIPRQTDF NLMRVTCRFQ EKCKQMKEDL ARMHFEKFEK
IQKLEEEQRH FNIEKQRLNR RRQNEIMAKI QAAIIQIPKP EPNRTLKAIE AQKLMKKKKE
AEDVANEIKK FEALIKKDLQ TRESISKTSL DTIDQTAAEL LNTYSDDDYI KKIQKRLEED
AFAREQREKR RRRLLMDQLI AHEAQEEAYR EEQLIHRLMR QSQQERRIAV QLMHVRHEKE
VLWQNRIFRE KQFEERRLKD FQDALDREAA LTKQAKIDFA EQTLRAKEIH EKIAMERAQQ
RYEKHYGICA EILDQILDLC TKVADYRLLT NNLIPHKMMR DWKELFFSGN PIYEQTSHTH
GQTESTEDHH VELNKRNLLD SKDYEDYKNM TGEWALPEDM ANSSPPSNNN ILGHVLLRLI
ENSYPSEKEV ADTELPSFAV KGCLLGKTLS GKTTILKALQ NDIPIQVLSI DTLVQEAIQA
FNDGEKSSKA PSMHEDVKGD PEVDQKEMII PQDKNELTDT QVPGEAAPQK EGTKSSDFEQ
FRPSNSFLSL SMRAQLGAKS ELMLRRGKSV PDVLLVNILV NAIKKIPVNQ SWVLDGFPMT
VNQAKLLEEA LTGYKRKSLQ LKKKKAQMPT LALSPTTSKE VPFLPSAFDF AILLDISDNS
SLARINDIIA KEISHEISHE NVSGPTITSS EEKSEDRNLR DHIQHRIVGF LDNWPLLEEW
FTEPRNILKK VNAEIDEAFL CQKIKEIFAT EVSNKKIKVE KKLEEKETEK KSAVSPTEPP
TPTPPLASEA EKEKEVHQAK PSGKGKTQSV SPKGKAQGGK ISVKKSPIDS TEVSPTLIAM
PTTKPGSEEW VYVTEPIPEE LPSFLVPYWE LIEKTYISHI KTVLRQLRES QHTVLSYLYE
TRTSFQEFLR RPDHKQDFVS QWQADFNSVP EDLWEDEETK AELHQRVNDL RDRLWDICEA
RKEEAEQERL DIINESWLQD YIGITMNHFF SLMQAEVNRF QDTKRLLQDY YRAMESRIPL
EDNKKFTRIP LVQLDGKEIS EHQLRIPLVP RISSSPENVS MRPKVKALLK GRFDPPLESL
EANFEGDEKI VLDTWQQASL AISQMVAAEV HQRLAEEEKE QPQLDPKEKS PQSGTNKKAK
KEKEQAKKEK EQAKKEKEQA KKEKEPPKKK MAEKKGKGKS SPVVEVSPVT ITPEEMAEME
RKNELKLKIK EEHLAALQTE EQATQFRLEL IKSKALSILE DLVTKVIEVY RFMEKWLGKR
YLNEMASIQK LTEVARYHIE TATKIQNEIY LSQEDFYING DIKVFPDPPP PTRPPPVEKE
ENGTLTIEQL DNLRDQFLDM APKGIIGNKA FTDILLDLVT LNLGTNNLSS SWMHLTQLDL
QEITSLLTVN TEFVDWRKFL MVAAMPWPIP LEEELLETLQ RFKATDEAQT GTITFEQYKQ
AGLWFSGDED IKIPENPLEP LPFNRQEHLI EFFFRLFADS EKDPPQLDYT QMLLYFACHP
DTLEGVYRAL SVAVGTHIFR QVETPMLTAE KTSISSVSPN EEFPEAEENS TREELKEEKD
ERDQKEEEIP ENANTEKISM ETLLKVFGGG NEVLDANRFA SYLKTENIYS ENLIKTFQDL
GAKNLEPIEV NILLKHPYIQ DLITNYVDYK IPDIKMILQR SEHAQGSDGE RSPSRLTDEK
K