ID   SPEFL_HAEIN             Reviewed;          64 AA.
AC   P44021;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 58.
DE   RecName: Full=Leader peptide SpeFL {ECO:0000255|HAMAP-Rule:MF_00851};
DE   AltName: Full=Arrest peptide SpeFL {ECO:0000255|HAMAP-Rule:MF_00851};
GN   Name=speFL {ECO:0000255|HAMAP-Rule:MF_00851}; OrderedLocusNames=HI_0592;
OS   Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=71421;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   PubMed=7542800; DOI=10.1126/science.7542800;
RA   Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA   Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA   McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA   Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA   Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA   Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA   Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA   Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT   "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT   Rd.";
RL   Science 269:496-512(1995).
CC   -!- FUNCTION: A small protein (arrest peptide) encoded upstream of
CC       inducible ornithine carboxylase gene (speF) that controls expression of
CC       downstream genes (usually speF and potE) by transcriptional and
CC       translational attenuation. {ECO:0000255|HAMAP-Rule:MF_00851}.
CC   -!- SUBUNIT: Binds ornithine in stalled 70S ribosomes, blocking the upper
CC       two-thirds of the exit tunnel. Contacts 23S rRNA and ribosomal proteins
CC       L4 and L22. {ECO:0000255|HAMAP-Rule:MF_00851}.
CC   -!- INDUCTION: Induced by ornithine, repressed by putrescine. Part of the
CC       speFL-speF-potE operon. {ECO:0000255|HAMAP-Rule:MF_00851}.
CC   -!- SIMILARITY: Belongs to the speF operon leader peptide family.
CC       {ECO:0000255|HAMAP-Rule:MF_00851}.
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DR   EMBL; L42023; AAC22259.1; -; Genomic_DNA.
DR   PIR; C64010; C64010.
DR   AlphaFoldDB; P44021; -.
DR   STRING; 71421.HI_0592; -.
DR   EnsemblBacteria; AAC22259; AAC22259; HI_0592.
DR   KEGG; hin:HI_0592; -.
DR   HOGENOM; CLU_2861483_0_0_6; -.
DR   Proteomes; UP000000579; Chromosome.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006448; P:regulation of translational elongation; IEA:UniProtKB-UniRule.
DR   GO; GO:0031556; P:transcriptional attenuation by ribosome; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00851; Leader_SpeFL; 1.
DR   InterPro; IPR021237; SpeFL.
DR   Pfam; PF10940; DUF2618; 1.
PE   3: Inferred from homology;
KW   Leader peptide; Reference proteome; RNA-binding; rRNA-binding;
KW   Transcription; Transcription regulation; Translation regulation.
FT   CHAIN           1..64
FT                   /note="Leader peptide SpeFL"
FT                   /id="PRO_0000077937"
FT   MOTIF           32..38
FT                   /note="Ornithine recognition loop"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00851"
FT   BINDING         35
FT                   /ligand="L-ornithine"
FT                   /ligand_id="ChEBI:CHEBI:46911"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00851"
SQ   SEQUENCE   64 AA;  7776 MW;  D058E8A5BAA2117D CRC64;
     MLFRTYIHRY VHTKALRFLR FNPIKGRSLM AHIRRTRHIM MPSYRSCFSY SLFASQNKPS
     NRAL