SPEM1_MOUSE
ID SPEM1_MOUSE Reviewed; 310 AA.
AC Q5F289; Q497J9; Q9D9D4;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2005, sequence version 1.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=Spermatid maturation protein 1;
GN Name=Spem1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND DISRUPTION PHENOTYPE.
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=17426145; DOI=10.1073/pnas.0701669104;
RA Zheng H., Stratton C.J., Morozumi K., Jin J., Yanagimachi R., Yan W.;
RT "Lack of Spem1 causes aberrant cytoplasm removal, sperm deformation, and
RT male infertility.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:6852-6857(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Required for proper cytoplasm removal during spermatogenesis.
CC {ECO:0000269|PubMed:17426145}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}. Cytoplasm {ECO:0000269|PubMed:17426145}.
CC -!- TISSUE SPECIFICITY: Testis-specific. Exclusively present in cytoplasm
CC of steps 14-16 elongated spermatids (at protein level).
CC {ECO:0000269|PubMed:17426145}.
CC -!- DISRUPTION PHENOTYPE: Mice are completely infertile because of deformed
CC sperm characterized by a bent head wrapped around by the neck and the
CC middle piece of the tail. Lack of Spem1 causes failure of the cytoplasm
CC to become loose and detach from the head and the neck region of the
CC developing spermatozoa. {ECO:0000269|PubMed:17426145}.
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DR EMBL; EF120626; ABO33756.1; -; mRNA.
DR EMBL; AK007080; BAB24854.1; -; mRNA.
DR EMBL; AL603707; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC100505; AAI00506.1; -; mRNA.
DR CCDS; CCDS36199.1; -.
DR RefSeq; NP_083131.1; NM_028855.1.
DR AlphaFoldDB; Q5F289; -.
DR BioGRID; 216637; 1.
DR STRING; 10090.ENSMUSP00000037500; -.
DR iPTMnet; Q5F289; -.
DR PhosphoSitePlus; Q5F289; -.
DR PaxDb; Q5F289; -.
DR PRIDE; Q5F289; -.
DR ProteomicsDB; 257347; -.
DR Antibodypedia; 2661; 114 antibodies from 17 providers.
DR Ensembl; ENSMUST00000045771; ENSMUSP00000037500; ENSMUSG00000041165.
DR GeneID; 74288; -.
DR KEGG; mmu:74288; -.
DR UCSC; uc007jrv.1; mouse.
DR CTD; 374768; -.
DR MGI; MGI:1921538; Spem1.
DR VEuPathDB; HostDB:ENSMUSG00000041165; -.
DR eggNOG; ENOG502ST8J; Eukaryota.
DR GeneTree; ENSGT00510000049389; -.
DR HOGENOM; CLU_078808_0_0_1; -.
DR InParanoid; Q5F289; -.
DR OMA; PAICSYH; -.
DR OrthoDB; 1151298at2759; -.
DR PhylomeDB; Q5F289; -.
DR TreeFam; TF338031; -.
DR BioGRID-ORCS; 74288; 3 hits in 71 CRISPR screens.
DR ChiTaRS; Spem1; mouse.
DR PRO; PR:Q5F289; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q5F289; protein.
DR Bgee; ENSMUSG00000041165; Expressed in testis and 24 other tissues.
DR Genevisible; Q5F289; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0030317; P:flagellated sperm motility; IMP:MGI.
DR GO; GO:0007291; P:sperm individualization; IMP:MGI.
DR GO; GO:0007283; P:spermatogenesis; IMP:MGI.
DR InterPro; IPR031368; SPEM1_N.
DR Pfam; PF15670; Spem1; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; Developmental protein; Differentiation; Membrane;
KW Reference proteome; Spermatogenesis; Transmembrane; Transmembrane helix.
FT CHAIN 1..310
FT /note="Spermatid maturation protein 1"
FT /id="PRO_0000307807"
FT TRANSMEM 29..49
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 215..238
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 262..286
FT /evidence="ECO:0000255"
FT CONFLICT 52
FT /note="R -> Q (in Ref. 2; BAB24854)"
FT /evidence="ECO:0000305"
FT CONFLICT 213
FT /note="P -> L (in Ref. 4; AAI00506)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 310 AA; 35062 MW; F5AC1EB7A91864BC CRC64;
MAMAERPRPG WASYQNPNIN NCQDMGNSIL LLLGLIVCIN IGINLVTLLW SRIRILLHRM
FHIICEKETS TSLGKLVQPL RKQTYPKVHL RCTMDPVKMT VTPPPTRRRY RHRAPPSRRA
RCPIAWAPDT DDEKPPHQHP AICSRHWNDS KDWEGFQSMQ EVWEPWTQDG LEQPPQTIRF
QPAVEARPLK SEIRSDQGLE AYVYTVNPPP PSPEALSHKN NAAGSGGCVE GEQAQGQPVS
PSFLGPANVP EIPRRHSSGR IVYDARDVRR RLRELTQEVE ALSHCYPLVS GSSAAEGTGK
DWVYRPLKGR
