SPEM2_MOUSE
ID SPEM2_MOUSE Reviewed; 503 AA.
AC Q8C5U4;
DT 03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Uncharacterized protein SPEM2;
GN Name=Spem2 {ECO:0000312|MGI:MGI:1918293};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
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DR EMBL; AK077090; BAC36606.1; -; mRNA.
DR EMBL; AL603707; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC137961; AAI37962.1; -; mRNA.
DR CCDS; CCDS24912.1; -.
DR RefSeq; NP_780577.1; NM_175368.1.
DR AlphaFoldDB; Q8C5U4; -.
DR STRING; 10090.ENSMUSP00000051204; -.
DR iPTMnet; Q8C5U4; -.
DR PhosphoSitePlus; Q8C5U4; -.
DR PaxDb; Q8C5U4; -.
DR PRIDE; Q8C5U4; -.
DR ProteomicsDB; 263319; -.
DR Antibodypedia; 6127; 67 antibodies from 16 providers.
DR DNASU; 108803; -.
DR Ensembl; ENSMUST00000056941; ENSMUSP00000051204; ENSMUSG00000044084.
DR GeneID; 108803; -.
DR KEGG; mmu:108803; -.
DR UCSC; uc007jru.1; mouse.
DR CTD; 201243; -.
DR MGI; MGI:1918293; Spem2.
DR VEuPathDB; HostDB:ENSMUSG00000044084; -.
DR eggNOG; ENOG502RKNT; Eukaryota.
DR GeneTree; ENSGT00510000049558; -.
DR HOGENOM; CLU_045547_0_0_1; -.
DR InParanoid; Q8C5U4; -.
DR OMA; DVHIHCT; -.
DR OrthoDB; 646751at2759; -.
DR PhylomeDB; Q8C5U4; -.
DR TreeFam; TF337389; -.
DR BioGRID-ORCS; 108803; 2 hits in 71 CRISPR screens.
DR PRO; PR:Q8C5U4; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q8C5U4; protein.
DR Bgee; ENSMUSG00000044084; Expressed in testis and 3 other tissues.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR InterPro; IPR031368; SPEM1_N.
DR Pfam; PF15670; Spem1; 1.
PE 2: Evidence at transcript level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..503
FT /note="Uncharacterized protein SPEM2"
FT /id="PRO_0000387570"
FT TRANSMEM 26..46
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 155..176
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 311..381
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 436..456
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 472..503
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 345..374
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 475..497
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 503 AA; 56934 MW; 564AE39AE38ECE19 CRC64;
MENQLWQNTL RCCEQYQESP QDAENILFLL LGLIILVNIS INVATAMWQG LQNAIDKMIF
WMNQKTEVVQ VTECPPKEPQ PANVQDVHIH CILDPVQVKM AQPTQCSSSS SHYFCKRSND
RRSRRRYGYQ QGNLQIHQSS QQQGCLSHQQ RLRNRPLSRG YPPFRKQPQG HKMSQMRPMP
FFDMEDRDSL PEGHSCPHAK QPRRGWGSLC KPVRLASNVG LWGRQGGILA SLPLPSLYLS
PELRRLPKRV EAKSELRLQG FGPHYSQSRI WGTVEAEQWA SSPPPPRRLL PNPSWVTVGY
SSFPSGGHIP YDARDQWRRG TEGCEPPPAF VPRNPREVQG YRDHNSQAHR QNFSSHTHSQ
PNHSPPQSMG HVGYSSRESH EVRRRAPDWI EVFPSRHPLT TSTSLTALGE ASYQRAPPAS
SGLMIPHSSQ RLAEVQISDP TPPPTTFVPL SRNPGGNATY QVYDSLELKR QVQENRGRAS
SLPPPSTSAS RPSLHRSRTG KLN
