SPER3_ARATH
ID SPER3_ARATH Reviewed; 193 AA.
AC Q6IDB0; Q9SVV1;
DT 13-APR-2016, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Protein SPEAR3 {ECO:0000303|PubMed:25527103};
DE AltName: Full=SPL-like, EAR-containing protein 3 {ECO:0000303|PubMed:25527103};
DE AltName: Full=TCP interactor containing EAR motif protein 1 {ECO:0000303|PubMed:23444332};
GN Name=SPEAR3 {ECO:0000303|PubMed:25527103};
GN Synonyms=TIE1 {ECO:0000303|PubMed:23444332};
GN OrderedLocusNames=At4g28840 {ECO:0000312|Araport:AT4G28840};
GN ORFNames=F16A16.50 {ECO:0000312|EMBL:CAA22968.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:AAT41746.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Cheuk R., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF LEU-189; LEU-191 AND
RP LEU-193, DOMAIN, GENE FAMILY, NOMENCLATURE, INTERACTION WITH TPL; TPR1;
RP TPR2; TPR3; TPR4; TCP2; TCP3; TCP4; TCP5; TCP10; TCP13; TCP17 AND TCP24,
RP TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=23444332; DOI=10.1105/tpc.113.109223;
RA Tao Q., Guo D., Wei B., Zhang F., Pang C., Jiang H., Zhang J., Wei T.,
RA Gu H., Qu L.J., Qin G.;
RT "The TIE1 transcriptional repressor links TCP transcription factors with
RT TOPLESS/TOPLESS-RELATED corepressors and modulates leaf development in
RT Arabidopsis.";
RL Plant Cell 25:421-437(2013).
RN [5]
RP GENE FAMILY, NOMENCLATURE, AND INTERACTION WITH SPL AND SPEAR2.
RX PubMed=25527103; DOI=10.1016/j.jgg.2014.08.009;
RA Chen G.H., Sun J.Y., Liu M., Liu J., Yang W.C.;
RT "SPOROCYTELESS is a novel embryophyte-specific transcription repressor that
RT interacts with TPL and TCP proteins in Arabidopsis.";
RL J. Genet. Genomics 41:617-625(2014).
CC -!- FUNCTION: Transcriptional regulator of leaf development. Acts as an
CC adapter-like transcriptional repressor recruiting TPL/TPR corepressors
CC to inhibit the CIN-like TCP transcription factors (PubMed:23444332).
CC {ECO:0000269|PubMed:23444332}.
CC -!- SUBUNIT: Interacts with TPL and the TPR corepressors TPR1, TPR2, TPR3,
CC TPR4, and with the TCP transcription factors TCP2, TCP3, TCP4, TCP5,
CC TCP10, TCP13, TCP17 and TCP24 (PubMed:23444332). Interacts with SPL and
CC SPEAR2 (PubMed:25527103). {ECO:0000269|PubMed:23444332,
CC ECO:0000269|PubMed:25527103}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:23444332}.
CC -!- TISSUE SPECIFICITY: Expressed in shoot apical meristem, cotyledons and
CC leaves. Detected at the leaf margins and in the vascular bundles at the
CC base of the leaves. {ECO:0000269|PubMed:23444332}.
CC -!- DOMAIN: Contains 1 EAR motif required for the interaction with TPL and
CC TPRs. {ECO:0000269|PubMed:23444332}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype, due to the redundancy with
CC SPEAR2/TIE4 and SPEAR4/TIE3. {ECO:0000269|PubMed:23444332}.
CC -!- MISCELLANEOUS: Knock down expression of SPEAR2/TIE4 and SPEAR4/TIE3 by
CC RNAi in a SPEAR3/TIE1 null mutant produces lines displaying epinastic
CC leaves. {ECO:0000269|PubMed:23444332}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA22968.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB81472.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL035353; CAA22968.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161573; CAB81472.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE85553.1; -; Genomic_DNA.
DR EMBL; BT014763; AAT41746.1; -; mRNA.
DR EMBL; BT015002; AAT70453.1; -; mRNA.
DR PIR; T04515; T04515.
DR RefSeq; NP_194613.4; NM_119028.5.
DR AlphaFoldDB; Q6IDB0; -.
DR STRING; 3702.AT4G28840.1; -.
DR PaxDb; Q6IDB0; -.
DR PRIDE; Q6IDB0; -.
DR ProteomicsDB; 232569; -.
DR EnsemblPlants; AT4G28840.1; AT4G28840.1; AT4G28840.
DR GeneID; 829005; -.
DR Gramene; AT4G28840.1; AT4G28840.1; AT4G28840.
DR KEGG; ath:AT4G28840; -.
DR Araport; AT4G28840; -.
DR TAIR; locus:2117828; AT4G28840.
DR eggNOG; ENOG502RXQS; Eukaryota.
DR HOGENOM; CLU_094368_0_0_1; -.
DR InParanoid; Q6IDB0; -.
DR OrthoDB; 1441325at2759; -.
DR PhylomeDB; Q6IDB0; -.
DR PRO; PR:Q6IDB0; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q6IDB0; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0048366; P:leaf development; IMP:TAIR.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:TAIR.
DR InterPro; IPR040356; SPEAR.
DR PANTHER; PTHR33388; PTHR33388; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..193
FT /note="Protein SPEAR3"
FT /id="PRO_0000435868"
FT REGION 1..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 85..104
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 44..52
FT /note="SPL"
FT /evidence="ECO:0000305"
FT MOTIF 187..193
FT /note="EAR"
FT /evidence="ECO:0000305"
FT COMPBIAS 9..30
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 189
FT /note="L->S: Loss of repression activities; when associated
FT with S-191 and Ser-193."
FT /evidence="ECO:0000269|PubMed:23444332"
FT MUTAGEN 191
FT /note="L->S: Loss of repression activities; when associated
FT with S-189 and Ser-193."
FT /evidence="ECO:0000269|PubMed:23444332"
FT MUTAGEN 193
FT /note="L->S: Loss of repression activities; when associated
FT with S-189 and Ser-191."
FT /evidence="ECO:0000269|PubMed:23444332"
SQ SEQUENCE 193 AA; 21409 MW; CDFEF447D6E47519 CRC64;
MGSSFFGRPK MGGSSSSSPT SSSSSPAKRG KNKNGSDKPK QPQRGLGVAQ LEKIRLHGEY
NCNSFNTYPS YHPSTYQEDV RIQGGYPSIP SSSPSFSYAS SSPPPAPYGF HPNMMMNANN
DQYERTTIRY GDSQPHRAPS WNPSYGILES QHFVEPNTTR HFLHEDQRNI SLGSGIQNFE
TSEANELDLE LRL
