SPER4_ARATH
ID SPER4_ARATH Reviewed; 300 AA.
AC Q9SHQ9; A0ME99;
DT 13-APR-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Protein SPEAR4 {ECO:0000303|PubMed:25527103};
DE AltName: Full=SPL-like, EAR-containing protein 4 {ECO:0000303|PubMed:25527103};
DE AltName: Full=TCP interactor containing EAR motif protein 3 {ECO:0000303|PubMed:23444332};
GN Name=SPEAR4 {ECO:0000303|PubMed:25527103};
GN Synonyms=TIE3 {ECO:0000303|PubMed:23444332};
GN OrderedLocusNames=At1g29010 {ECO:0000312|Araport:AT1G29010};
GN ORFNames=F1K23.3 {ECO:0000312|EMBL:AAF24558.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [4]
RP GENE FAMILY, NOMENCLATURE, AND TISSUE SPECIFICITY.
RX PubMed=23444332; DOI=10.1105/tpc.113.109223;
RA Tao Q., Guo D., Wei B., Zhang F., Pang C., Jiang H., Zhang J., Wei T.,
RA Gu H., Qu L.J., Qin G.;
RT "The TIE1 transcriptional repressor links TCP transcription factors with
RT TOPLESS/TOPLESS-RELATED corepressors and modulates leaf development in
RT Arabidopsis.";
RL Plant Cell 25:421-437(2013).
RN [5]
RP GENE FAMILY, NOMENCLATURE, AND INTERACTION WITH SPL AND SPEAR2.
RX PubMed=25527103; DOI=10.1016/j.jgg.2014.08.009;
RA Chen G.H., Sun J.Y., Liu M., Liu J., Yang W.C.;
RT "SPOROCYTELESS is a novel embryophyte-specific transcription repressor that
RT interacts with TPL and TCP proteins in Arabidopsis.";
RL J. Genet. Genomics 41:617-625(2014).
CC -!- FUNCTION: Adapter-like transcriptional repressor recruiting TPL/TPR
CC coepressors to inhibit TCP transcription factors (By similarity). May
CC be involved in leaf development. {ECO:0000250|UniProtKB:Q6IDB0,
CC ECO:0000305|PubMed:23444332}.
CC -!- SUBUNIT: Interacts with SPL and SPEAR2. {ECO:0000269|PubMed:25527103}.
CC -!- TISSUE SPECIFICITY: Expressed in leaves. {ECO:0000269|PubMed:23444332}.
CC -!- MISCELLANEOUS: Knock down expression of SPEAR2/TIE4 and SPEAR4/TIE3 by
CC RNAi in a SPEAR3/TIE1 null mutant produces lines displaying epinastic
CC leaves. {ECO:0000269|PubMed:23444332}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABK28421.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC007508; AAF24558.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31028.1; -; Genomic_DNA.
DR EMBL; DQ446305; ABE65669.1; -; mRNA.
DR EMBL; DQ652868; ABK28421.1; ALT_SEQ; mRNA.
DR RefSeq; NP_174196.2; NM_102642.3.
DR AlphaFoldDB; Q9SHQ9; -.
DR STRING; 3702.AT1G29010.1; -.
DR iPTMnet; Q9SHQ9; -.
DR PaxDb; Q9SHQ9; -.
DR PRIDE; Q9SHQ9; -.
DR DNASU; 839775; -.
DR EnsemblPlants; AT1G29010.1; AT1G29010.1; AT1G29010.
DR GeneID; 839775; -.
DR Gramene; AT1G29010.1; AT1G29010.1; AT1G29010.
DR KEGG; ath:AT1G29010; -.
DR Araport; AT1G29010; -.
DR TAIR; locus:2018778; AT1G29010.
DR eggNOG; ENOG502R7WF; Eukaryota.
DR HOGENOM; CLU_919369_0_0_1; -.
DR InParanoid; Q9SHQ9; -.
DR OMA; HYLPPMN; -.
DR OrthoDB; 1138412at2759; -.
DR PhylomeDB; Q9SHQ9; -.
DR PRO; PR:Q9SHQ9; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SHQ9; baseline and differential.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0048366; P:leaf development; IMP:TAIR.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:TAIR.
DR InterPro; IPR040356; SPEAR.
DR PANTHER; PTHR33388; PTHR33388; 1.
PE 1: Evidence at protein level;
KW Reference proteome; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..300
FT /note="Protein SPEAR4"
FT /id="PRO_0000435869"
FT REGION 1..45
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 40..48
FT /note="SPL"
FT /evidence="ECO:0000305"
FT MOTIF 294..300
FT /note="EAR"
FT /evidence="ECO:0000305"
FT COMPBIAS 1..16
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 300 AA; 33603 MW; 5E3BBC969F4EB029 CRC64;
MCSKTSSVSY GNREDDDNYS SLCPKKQKHN NGGKKRVPRR GPGVAELEKI RLGEQHISTA
APFTLHHPPS LEKSPTITDR TGLVYPFSSY FSAGSFPSDL IPPAPVFQRK HDSSLHYLPP
MNLPKQGSGG FYQFIEPPSS QTSSLDNVTK FLDEEKISSA KRPWHFMADT AKCSVGPSTT
ISRDAKQTRS LDLRLKNHVQ DSGTTIRNPI TIDSPSSASP PTTIFANPSL GFPRFLQKEE
DDHEIIQRKS GTNFPLNKKP FYSFLPANDQ IIRDQDRSFS LRTERYDTVP DHGIDLRLKL
