SPERI_HUMAN
ID SPERI_HUMAN Reviewed; 591 AA.
AC Q76KD6; B4DWW9; Q5U5I8; Q7Z6L7;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 2.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Speriolin;
DE AltName: Full=Spermatogenesis and centriole-associated protein 1;
DE AltName: Full=Spermatogenesis-associated protein 15;
DE AltName: Full=Spermatogenic cell-specific Cdc20-binding protein;
GN Name=SPATC1; Synonyms=SPATA15, SPRN;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=15280373; DOI=10.1074/jbc.m403190200;
RA Goto M., Eddy E.M.;
RT "Speriolin is a novel spermatogenic cell-specific centrosomal protein
RT associated with the seventh WD motif of Cdc20.";
RL J. Biol. Chem. 279:42128-42138(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16421571; DOI=10.1038/nature04406;
RA Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M.,
RA Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L.,
RA Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S.,
RA Asakawa T., Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A.,
RA Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III,
RA Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K.,
RA Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P.,
RA Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H.,
RA Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B.,
RA O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K.,
RA Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L.,
RA Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G.,
RA Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W.,
RA Platzer M., Shimizu N., Lander E.S.;
RT "DNA sequence and analysis of human chromosome 8.";
RL Nature 439:331-335(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=20542897; DOI=10.1093/humrep/deq138;
RA Goto M., O'Brien D.A., Eddy E.M.;
RT "Speriolin is a novel human and mouse sperm centrosome protein.";
RL Hum. Reprod. 25:1884-1894(2010).
CC -!- SUBUNIT: Found in a complex with CDC20, CDC27 and TUBG1. Interacts with
CC CDC20 (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Cytoplasm, cytoskeleton,
CC microtubule organizing center, centrosome {ECO:0000250}.
CC Note=Colocalizes with the centrosomal pericentrin protein PCNT1 (By
CC similarity). Located in the connecting piece of sperm. {ECO:0000250,
CC ECO:0000269|PubMed:20542897}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q76KD6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q76KD6-2; Sequence=VSP_042815, VSP_042816;
CC -!- TISSUE SPECIFICITY: Detected only in testis.
CC {ECO:0000269|PubMed:20542897}.
CC -!- SIMILARITY: Belongs to the speriolin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH50390.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAH53547.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AB092352; BAD08232.1; -; mRNA.
DR EMBL; AK301710; BAG63181.1; -; mRNA.
DR EMBL; AC109322; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC050390; AAH50390.1; ALT_INIT; mRNA.
DR EMBL; BC053547; AAH53547.1; ALT_INIT; mRNA.
DR CCDS; CCDS47937.1; -. [Q76KD6-2]
DR CCDS; CCDS6413.2; -. [Q76KD6-1]
DR RefSeq; NP_001127846.1; NM_001134374.1. [Q76KD6-2]
DR RefSeq; NP_940974.2; NM_198572.2. [Q76KD6-1]
DR AlphaFoldDB; Q76KD6; -.
DR SMR; Q76KD6; -.
DR BioGRID; 131991; 4.
DR STRING; 9606.ENSP00000366690; -.
DR iPTMnet; Q76KD6; -.
DR PhosphoSitePlus; Q76KD6; -.
DR BioMuta; SPATC1; -.
DR DMDM; 162416255; -.
DR EPD; Q76KD6; -.
DR MassIVE; Q76KD6; -.
DR PaxDb; Q76KD6; -.
DR PeptideAtlas; Q76KD6; -.
DR PRIDE; Q76KD6; -.
DR ProteomicsDB; 68675; -. [Q76KD6-1]
DR ProteomicsDB; 68676; -. [Q76KD6-2]
DR Antibodypedia; 43621; 106 antibodies from 21 providers.
DR DNASU; 375686; -.
DR Ensembl; ENST00000377470.8; ENSP00000366690.3; ENSG00000186583.12. [Q76KD6-1]
DR Ensembl; ENST00000447830.2; ENSP00000387613.2; ENSG00000186583.12. [Q76KD6-2]
DR GeneID; 375686; -.
DR KEGG; hsa:375686; -.
DR MANE-Select; ENST00000377470.8; ENSP00000366690.3; NM_198572.3; NP_940974.2.
DR UCSC; uc011lkw.3; human. [Q76KD6-1]
DR CTD; 375686; -.
DR DisGeNET; 375686; -.
DR GeneCards; SPATC1; -.
DR HGNC; HGNC:30510; SPATC1.
DR HPA; ENSG00000186583; Tissue enriched (testis).
DR MIM; 610874; gene.
DR neXtProt; NX_Q76KD6; -.
DR OpenTargets; ENSG00000186583; -.
DR PharmGKB; PA134907641; -.
DR VEuPathDB; HostDB:ENSG00000186583; -.
DR eggNOG; ENOG502RYNF; Eukaryota.
DR GeneTree; ENSGT00520000055666; -.
DR HOGENOM; CLU_033431_1_0_1; -.
DR InParanoid; Q76KD6; -.
DR OMA; NDHKLDE; -.
DR OrthoDB; 827195at2759; -.
DR PhylomeDB; Q76KD6; -.
DR TreeFam; TF329273; -.
DR PathwayCommons; Q76KD6; -.
DR SignaLink; Q76KD6; -.
DR BioGRID-ORCS; 375686; 10 hits in 1070 CRISPR screens.
DR ChiTaRS; SPATC1; human.
DR GenomeRNAi; 375686; -.
DR Pharos; Q76KD6; Tdark.
DR PRO; PR:Q76KD6; -.
DR Proteomes; UP000005640; Chromosome 8.
DR RNAct; Q76KD6; protein.
DR Bgee; ENSG00000186583; Expressed in right testis and 86 other tissues.
DR ExpressionAtlas; Q76KD6; baseline and differential.
DR Genevisible; Q76KD6; HS.
DR GO; GO:0005813; C:centrosome; IDA:MGI.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR InterPro; IPR026715; SPATC1.
DR InterPro; IPR029384; Speriolin_C.
DR InterPro; IPR029385; Speriolin_N.
DR PANTHER; PTHR22192; PTHR22192; 2.
DR Pfam; PF15059; Speriolin_C; 1.
DR Pfam; PF15058; Speriolin_N; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Coiled coil; Cytoplasm; Cytoskeleton;
KW Reference proteome.
FT CHAIN 1..591
FT /note="Speriolin"
FT /id="PRO_0000312300"
FT REGION 1..78
FT /note="Necessary for targeting centrosomes"
FT /evidence="ECO:0000250"
FT REGION 302..331
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 346..435
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 1..42
FT /evidence="ECO:0000255"
FT COMPBIAS 346..375
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 387..412
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 413..435
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 436..441
FT /note="ESKQLA -> GFPEPQ (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_042815"
FT VAR_SEQ 442..591
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_042816"
FT CONFLICT 65
FT /note="S -> P (in Ref. 1; BAD08232 and 4; AAH50390)"
FT /evidence="ECO:0000305"
FT CONFLICT 473
FT /note="V -> A (in Ref. 4; AAH53547)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 591 AA; 62399 MW; 3604434BB5A6C20F CRC64;
MSLLTNYEGL RHQIERLVRE NEELKKLVRL IRENHELKSA IKTQAGGLGI SGFTSGLGEA
TAGLSSRQNN GVFLPPSPAV ANERVLEEVG IMALAPLAEM LTSLQPSATP GSLMSPLTGT
LSTLLSGPAP TSQSSPLTSF LTSPIAGPLT GTLASSLGLP STGTLTPSSL VAGPVAMSQS
SPLIAPVMGT VAVSLSSPLL SSTATPPGVS QNLLANPMSN LVLPEAPRLR LAEPLRGGPT
GPQSPACVVP TATTKVPLST EPPQSTQDPE PLSMAFAGAP LQTSTPIGAM GTPAPKTAFS
FNTSDTQAQP SAAQEQVVPA SVPTSPTTSP TVTVLASAPA LAPQVATSYT PSSTTHIAQG
APHPPSRMHN SPTQNLPVPH CPPHNAHSPP RTSSSPASVN DSRGPRTTEP STKSMMEVER
KLAHRKTSKF PENPRESKQL AWERLVGEIA FQLDRRILSS IFPERVRLYG FTVSNIPEKI
IQASLNPSDH KLDEKLCQRL TQRYVSVMNR LQSLGYNGRV HPALTEQLVN AYGILRERPE
LAASEGGPYT VDFLQRVVVE TVHPGMLADA LLLLSCLSQL AHDDGKPMFI W
