SPERI_MOUSE
ID SPERI_MOUSE Reviewed; 480 AA.
AC Q148B6; Q14AS6; Q76MV2; Q9D9F0;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2006, sequence version 1.
DT 25-MAY-2022, entry version 83.
DE RecName: Full=Speriolin;
DE AltName: Full=Spermatogenesis and centriole-associated protein 1;
DE AltName: Full=Spermatogenic cell-specific Cdc20-binding protein;
GN Name=Spatc1; Synonyms=Sprn;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH CDC20,
RP IDENTIFICATION IN A COMPLEX WITH CDC20; CDC27 AND TUBG1, MUTAGENESIS OF
RP LYS-302; VAL-305; ARG-314 AND LEU-469, SUBCELLULAR LOCATION, DEVELOPMENTAL
RP STAGE, AND TISSUE SPECIFICITY.
RC TISSUE=Testis;
RX PubMed=15280373; DOI=10.1074/jbc.m403190200;
RA Goto M., Eddy E.M.;
RT "Speriolin is a novel spermatogenic cell-specific centrosomal protein
RT associated with the seventh WD motif of Cdc20.";
RL J. Biol. Chem. 279:42128-42138(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=20542897; DOI=10.1093/humrep/deq138;
RA Goto M., O'Brien D.A., Eddy E.M.;
RT "Speriolin is a novel human and mouse sperm centrosome protein.";
RL Hum. Reprod. 25:1884-1894(2010).
CC -!- SUBUNIT: Found in a complex with CDC20, CDC27 and TUBG1. Interacts with
CC CDC20. {ECO:0000269|PubMed:15280373}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:15280373}.
CC Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
CC {ECO:0000269|PubMed:15280373, ECO:0000269|PubMed:20542897}.
CC Note=Colocalizes with the centrosomal pericentrin protein PCNT1.
CC Located in the connecting piece of sperm.
CC {ECO:0000269|PubMed:20542897}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q148B6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q148B6-2; Sequence=VSP_029818;
CC -!- TISSUE SPECIFICITY: Expressed in testis. Expressed in pachyten
CC spermatocytes, spermatids and epididymal sperm (at protein level).
CC {ECO:0000269|PubMed:15280373, ECO:0000269|PubMed:20542897}.
CC -!- SIMILARITY: Belongs to the speriolin family. {ECO:0000305}.
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DR EMBL; AB032199; BAD08243.1; -; mRNA.
DR EMBL; AK006994; BAB24825.1; -; mRNA.
DR EMBL; BC116725; AAI16726.1; -; mRNA.
DR EMBL; BC118509; AAI18510.1; -; mRNA.
DR CCDS; CCDS27564.1; -. [Q148B6-1]
DR RefSeq; NP_083128.1; NM_028852.1.
DR RefSeq; XP_017172256.1; XM_017316767.1. [Q148B6-2]
DR RefSeq; XP_017172257.1; XM_017316768.1. [Q148B6-2]
DR RefSeq; XP_017172258.1; XM_017316769.1. [Q148B6-2]
DR AlphaFoldDB; Q148B6; -.
DR SMR; Q148B6; -.
DR STRING; 10090.ENSMUSP00000073805; -.
DR iPTMnet; Q148B6; -.
DR PhosphoSitePlus; Q148B6; -.
DR PaxDb; Q148B6; -.
DR PRIDE; Q148B6; -.
DR ProteomicsDB; 261570; -. [Q148B6-1]
DR ProteomicsDB; 261571; -. [Q148B6-2]
DR DNASU; 74281; -.
DR GeneID; 74281; -.
DR KEGG; mmu:74281; -.
DR UCSC; uc007wjm.1; mouse. [Q148B6-1]
DR CTD; 375686; -.
DR MGI; MGI:1921531; Spatc1.
DR eggNOG; ENOG502RYNF; Eukaryota.
DR InParanoid; Q148B6; -.
DR OrthoDB; 827195at2759; -.
DR PhylomeDB; Q148B6; -.
DR TreeFam; TF329273; -.
DR BioGRID-ORCS; 74281; 0 hits in 72 CRISPR screens.
DR ChiTaRS; Spatc1; mouse.
DR PRO; PR:Q148B6; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q148B6; protein.
DR GO; GO:0005813; C:centrosome; IDA:MGI.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0043015; F:gamma-tubulin binding; IDA:MGI.
DR InterPro; IPR026715; SPATC1.
DR InterPro; IPR029384; Speriolin_C.
DR InterPro; IPR029385; Speriolin_N.
DR PANTHER; PTHR22192; PTHR22192; 2.
DR Pfam; PF15059; Speriolin_C; 1.
DR Pfam; PF15058; Speriolin_N; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Cytoplasm; Cytoskeleton;
KW Reference proteome.
FT CHAIN 1..480
FT /note="Speriolin"
FT /id="PRO_0000312301"
FT REGION 1..76
FT /note="Necessary for targeting to centrosomes"
FT REGION 252..297
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 305..324
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 2..45
FT /evidence="ECO:0000255"
FT COMPBIAS 270..293
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..296
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_029818"
FT MUTAGEN 302
FT /note="K->R: Abolishes CDC20 binding; when associated with
FT Q-314."
FT /evidence="ECO:0000269|PubMed:15280373"
FT MUTAGEN 305
FT /note="V->M: Abolishes CDC20 binding."
FT /evidence="ECO:0000269|PubMed:15280373"
FT MUTAGEN 314
FT /note="R->Q: Abolishes CDC20 binding; when associated with
FT R-302."
FT /evidence="ECO:0000269|PubMed:15280373"
FT MUTAGEN 469
FT /note="L->P: Abolishes CDC20 binding."
FT /evidence="ECO:0000269|PubMed:15280373"
FT CONFLICT 71
FT /note="F -> L (in Ref. 2; BAB24825)"
FT /evidence="ECO:0000305"
FT CONFLICT 265
FT /note="R -> L (in Ref. 1; BAD08243 and 2; BAB24825)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 480 AA; 51418 MW; 2FC3C35379E59ABF CRC64;
MSLLTSYEGL RHQIERLVRE NEELKKLVRL IRENQELKSA IKTQAGGLCI SGFTGGLGEA
AAGPPQHQGV FLPPASAAAK EPCSEDLGMV ALAPLADMLN TPQLSPAAGS LVNPLAATLN
PLLSGQIPLL QNNQFANLVP CSMSNQLTNP TTVSPGVTLA SSLGLPSTGP LNSQMTSPMT
VPPGTTLASS LGLTSTGSLT TSSRLVGPLA VSQSSPIMAP LAGTVAVSLS SPLLSSTATP
LGVAQNVVPN PINNIGQPET PRVRRAEPTR GNFSGTSAYA GPAPTSKVND TRGSRVMEQS
RKNVVEMERK TPHRKSNKLP DNPRDTKQLV CERLVGEIAF QLDRRILSSI FPERVRLYGF
TVSNIPEKII QASLNPSNHK LDEDLCQTLT QRYVSIMNKL QSLGYNGRVH PALTEQLVNE
YGILRERPEL AASEGGCYTV DFLQRVLLET VHPSKLTDAL LLLSCLHQLS HDDGKPMFIW
