ID   SPESP_MACFA             Reviewed;         352 AA.
AC   Q95LJ2;
DT   08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=Sperm equatorial segment protein 1 {ECO:0000250|UniProtKB:Q6UW49};
DE   Flags: Precursor;
GN   Name=SPESP1 {ECO:0000250|UniProtKB:Q6UW49}; ORFNames=QtsA-21536;
OS   Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Cercopithecidae; Cercopithecinae; Macaca.
OX   NCBI_TaxID=9541;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=12498619; DOI=10.1186/1471-2164-3-36;
RA   Osada N., Hida M., Kusuda J., Tanuma R., Hirata M., Suto Y., Hirai M.,
RA   Terao K., Sugano S., Hashimoto K.;
RT   "Cynomolgus monkey testicular cDNAs for discovery of novel human genes in
RT   the human genome sequence.";
RL   BMC Genomics 3:36-36(2002).
CC   -!- FUNCTION: Involved in fertilization ability of sperm.
CC       {ECO:0000250|UniProtKB:Q9D5A0}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, acrosome
CC       {ECO:0000250|UniProtKB:Q6UW49}. Note=Small proacrosomal granules
CC       (during the Golgi phase), enlarged acrosomal vesicles (during the cap
CC       phase), acrosome (during the elongating phase), equatorial segment of
CC       the acrosome (during the maturation phase) (By similarity). After
CC       acrosome reaction localizes to the equatorial segment region in both
CC       noncapacitated and capacitated, acrosome-reacted sperm (By similarity).
CC       {ECO:0000250|UniProtKB:Q6UW49, ECO:0000250|UniProtKB:Q9D5A0}.
CC   -!- PTM: Glycosylated. In testis there are two predominant forms of 77- and
CC       67-kDa and a form of 47-kDa, whereas in epididymal sperm from caput,
CC       corpus, and cauda there are two forms of 47- and 43-kDa. Testis forms
CC       contain complex carbohydrate residues. Epididymal sperm forms are N-
CC       glycosylated. Then undergoes significant glycosylation in the testis
CC       and that the majority of these glycoconjugates are removed by the time
CC       sperm reach the caput epididymis. {ECO:0000250|UniProtKB:Q9D5A0}.
CC   -!- SIMILARITY: Belongs to the SPESP1 family. {ECO:0000305}.
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DR   EMBL; AB072796; BAB69765.1; -; mRNA.
DR   RefSeq; NP_001274210.1; NM_001287281.1.
DR   AlphaFoldDB; Q95LJ2; -.
DR   SMR; Q95LJ2; -.
DR   STRING; 9541.XP_005559968.1; -.
DR   Ensembl; ENSMFAT00000030723; ENSMFAP00000022594; ENSMFAG00000043000.
DR   GeneID; 102127931; -.
DR   CTD; 246777; -.
DR   VEuPathDB; HostDB:ENSMFAG00000043000; -.
DR   eggNOG; ENOG502SG7W; Eukaryota.
DR   GeneTree; ENSGT00390000005362; -.
DR   OMA; YKSQLLP; -.
DR   OrthoDB; 1348911at2759; -.
DR   Proteomes; UP000233100; Chromosome 7.
DR   Bgee; ENSMFAG00000043000; Expressed in skeletal muscle tissue and 13 other tissues.
DR   GO; GO:0001669; C:acrosomal vesicle; IEA:UniProtKB-SubCell.
DR   GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR   GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR   GO; GO:0007340; P:acrosome reaction; IEA:InterPro.
DR   GO; GO:0009566; P:fertilization; ISS:UniProtKB.
DR   GO; GO:0007342; P:fusion of sperm to egg plasma membrane involved in single fertilization; IEA:InterPro.
DR   GO; GO:0035036; P:sperm-egg recognition; ISS:UniProtKB.
DR   InterPro; IPR026743; Equatorial_segment.
DR   PANTHER; PTHR31667; PTHR31667; 1.
DR   Pfam; PF15754; SPESP1; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasmic vesicle; Developmental protein; Glycoprotein;
KW   Reference proteome; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..352
FT                   /note="Sperm equatorial segment protein 1"
FT                   /id="PRO_0000042708"
FT   REGION          140..203
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        159..201
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        128
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   352 AA;  39425 MW;  EE750D1946886FE1 CRC64;
     MKSLVLLVAL LLWSSSVPAY PSITVSPDEE QNLNHYIQVL ENLVLSVPPK EPGREKKSNS
     PKHVYSIASK GSKFKELITH GDTSTENDVL TNPISEETTT FPTGDFTPEI GMKKHMESTP
     FWSIKPNNVS IVLHAEEPYI EKEEPEPEPE PTARQTEAPR TLPVVTESST SPYVTSYKSP
     VTTSDRSTGI EISTESEDVP QLSGETAIEK SKELTFGKHP ESWNNDDILK KILDINSQVQ
     QALLSDTSNP AYREDIEASK EHLKRSLALA AAAEHKLETM YKSQLLSPGQ TSNKIDDIET
     VINMLCNSRS KLYEYLDIKY VPPEMREEAA TVFNTLKNMC RSRRVTALLK VY