ID   SPESP_RAT               Reviewed;         409 AA.
AC   A0JPP4;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   12-DEC-2006, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Sperm equatorial segment protein 1 {ECO:0000250|UniProtKB:Q6UW49};
DE   Flags: Precursor;
GN   Name=Spesp1 {ECO:0000312|RGD:1563468};
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1] {ECO:0000312|EMBL:AAI27528.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis {ECO:0000312|EMBL:AAI27528.1};
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Involved in fertilization ability of sperm.
CC       {ECO:0000250|UniProtKB:Q9D5A0}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, acrosome
CC       {ECO:0000250|UniProtKB:Q6UW49}. Note=Small proacrosomal granules
CC       (during the Golgi phase), enlarged acrosomal vesicles (during the cap
CC       phase), acrosome (during the elongating phase), equatorial segment of
CC       the acrosome (during the maturation phase) (By similarity). After
CC       acrosome reaction localizes to the equatorial segment region in both
CC       noncapacitated and capacitated, acrosome-reacted sperm (By similarity).
CC       {ECO:0000250|UniProtKB:Q6UW49, ECO:0000250|UniProtKB:Q9D5A0}.
CC   -!- PTM: Glycosylated. In testis there are two predominant forms of 77- and
CC       67-kDa and a form of 47-kDa, whereas in epididymal sperm from caput,
CC       corpus, and cauda there are two forms of 47- and 43-kDa. Testis forms
CC       contain complex carbohydrate residues. Epididymal sperm forms are N-
CC       glycosylated. Then undergoes significant glycosylation in the testis
CC       and that the majority of these glycoconjugates are removed by the time
CC       sperm reach the caput epididymis. {ECO:0000250|UniProtKB:Q9D5A0}.
CC   -!- SIMILARITY: Belongs to the SPESP1 family. {ECO:0000255}.
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DR   EMBL; BC127527; AAI27528.1; -; mRNA.
DR   RefSeq; NP_001071150.1; NM_001077682.1.
DR   AlphaFoldDB; A0JPP4; -.
DR   SMR; A0JPP4; -.
DR   STRING; 10116.ENSRNOP00000043936; -.
DR   GlyGen; A0JPP4; 1 site.
DR   PaxDb; A0JPP4; -.
DR   Ensembl; ENSRNOT00000049441; ENSRNOP00000043936; ENSRNOG00000025359.
DR   GeneID; 501010; -.
DR   KEGG; rno:501010; -.
DR   UCSC; RGD:1563468; rat.
DR   CTD; 246777; -.
DR   RGD; 1563468; Spesp1.
DR   eggNOG; ENOG502SG7W; Eukaryota.
DR   GeneTree; ENSGT00390000005362; -.
DR   HOGENOM; CLU_787463_0_0_1; -.
DR   InParanoid; A0JPP4; -.
DR   OMA; YKSQLLP; -.
DR   OrthoDB; 1348911at2759; -.
DR   PhylomeDB; A0JPP4; -.
DR   TreeFam; TF337441; -.
DR   PRO; PR:A0JPP4; -.
DR   Proteomes; UP000002494; Chromosome 8.
DR   Bgee; ENSRNOG00000025359; Expressed in testis and 7 other tissues.
DR   GO; GO:0001669; C:acrosomal vesicle; ISO:RGD.
DR   GO; GO:0007340; P:acrosome reaction; ISO:RGD.
DR   GO; GO:0009566; P:fertilization; ISS:UniProtKB.
DR   GO; GO:0007342; P:fusion of sperm to egg plasma membrane involved in single fertilization; ISO:RGD.
DR   GO; GO:0035036; P:sperm-egg recognition; ISS:UniProtKB.
DR   InterPro; IPR026743; Equatorial_segment.
DR   PANTHER; PTHR31667; PTHR31667; 1.
DR   Pfam; PF15754; SPESP1; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasmic vesicle; Developmental protein; Glycoprotein;
KW   Reference proteome; Signal.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   CHAIN           19..409
FT                   /note="Sperm equatorial segment protein 1"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000274537"
FT   REGION          141..223
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        155..195
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        196..223
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        132
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   409 AA;  45880 MW;  DF544BBF3F4C807F CRC64;
     MKPVVLVALL WLWPSSFLAY PTATVLPDEE QNLNHYVQIL QNLIMSVPTK EQGFGRKFRS
     SRTVDGAEAR SLSSEMLLTP GLVSAQEVTT PETEVMLTSM VEKTTFPSSG FTMESEKRRT
     HSKAFWSIQP NNVSIVLHTE EPYIEKEPEP EPELETEEES RRLATEPEPE PESESAPETE
     TRQTAESEEE VETSTAQNKV RTGTSRMSTV ITQTTNTQGT PNTVTVKTIN NLDVSTESED
     VPQLSGQSEI LNVEDLPGHH SLSTRHENIL RKISNINSQI HQGLLSDHSS PAYKEFIKAS
     REHLKRSLAL AAAAEHKLQQ MYGSNVFLDG HTSDPDDDME VIINMLYNSR SKLSDYFTIN
     HVPSELREKA TVVIAVLKKI LCVDQVEMQS LIRKLLSNNM KILNILNVP