ID   SPF27_DANRE             Reviewed;         225 AA.
AC   Q5RKQ0;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Pre-mRNA-splicing factor SPF27;
DE   AltName: Full=Protein BCAS2 homolog;
GN   Name=bcas2; ORFNames=zgc:101730;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Olfactory epithelium;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Required for pre-mRNA splicing as component of the activated
CC       spliceosome. May have a scaffolding role in the spliceosome assembly as
CC       it contacts all other components of the core complex.
CC       {ECO:0000250|UniProtKB:O75934}.
CC   -!- SUBUNIT: Component of the pre-catalytic and catalytic spliceosome
CC       complexes. Component of the postcatalytic spliceosome P complex.
CC       {ECO:0000250|UniProtKB:O75934}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O75934}.
CC   -!- SIMILARITY: Belongs to the SPF27 family. {ECO:0000305}.
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DR   EMBL; BC085427; AAH85427.1; -; mRNA.
DR   RefSeq; NP_001007775.1; NM_001007774.1.
DR   AlphaFoldDB; Q5RKQ0; -.
DR   SMR; Q5RKQ0; -.
DR   STRING; 7955.ENSDARP00000064065; -.
DR   PaxDb; Q5RKQ0; -.
DR   Ensembl; ENSDART00000064066; ENSDARP00000064065; ENSDARG00000043631.
DR   GeneID; 493614; -.
DR   KEGG; dre:493614; -.
DR   CTD; 10286; -.
DR   ZFIN; ZDB-GENE-030408-5; bcas2.
DR   eggNOG; KOG3096; Eukaryota.
DR   GeneTree; ENSGT00390000014494; -.
DR   HOGENOM; CLU_082523_2_1_1; -.
DR   InParanoid; Q5RKQ0; -.
DR   OMA; SHLSMRH; -.
DR   OrthoDB; 1607056at2759; -.
DR   PhylomeDB; Q5RKQ0; -.
DR   Reactome; R-DRE-72163; mRNA Splicing - Major Pathway.
DR   PRO; PR:Q5RKQ0; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 8.
DR   Bgee; ENSDARG00000043631; Expressed in mature ovarian follicle and 35 other tissues.
DR   GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0000974; C:Prp19 complex; IBA:GO_Central.
DR   GO; GO:0071007; C:U2-type catalytic step 2 spliceosome; ISS:UniProtKB.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR   InterPro; IPR008409; SPF27.
DR   PANTHER; PTHR13296; PTHR13296; 1.
DR   Pfam; PF05700; BCAS2; 1.
PE   2: Evidence at transcript level;
KW   Coiled coil; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW   Spliceosome.
FT   CHAIN           1..225
FT                   /note="Pre-mRNA-splicing factor SPF27"
FT                   /id="PRO_0000064860"
FT   COILED          138..222
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   225 AA;  25863 MW;  6F2BE9788B72F743 CRC64;
     MAGPASVAGD VFVDALPYFD QGYDATGVRE AAAALVEEET RRYRPTKNYL SYLPTPDFSA
     FETEIMRNEF ERLAARQPME LLSMKRYELP APSSGQKNDM TAWQDCVNNS MAQLEHQAVR
     IENLELMAQY GTNAWKMSND NLALMIENSQ KELQNVRKEI QDLNWQRKND QLAGGAKLRE
     LESNWVSLVS KNYEIERAIV QLENEVAQMK QQQGDENKEN IRQDF