ID   SPF27_DICDI             Reviewed;         226 AA.
AC   Q54SG7;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   18-MAR-2008, sequence version 2.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Pre-mRNA-splicing factor spf27;
GN   Name=spf27; Synonyms=bcas2; ORFNames=DDB_G0282763;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Required for pre-mRNA splicing as component of the activated
CC       spliceosome. {ECO:0000250|UniProtKB:O75934}.
CC   -!- SUBUNIT: Component of the pre-catalytic and catalytic spliceosome
CC       complexes. Component of the postcatalytic spliceosome P complex.
CC       {ECO:0000250|UniProtKB:O75934}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O75934}.
CC   -!- SIMILARITY: Belongs to the SPF27 family. {ECO:0000305}.
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DR   EMBL; AAFI02000047; EAL66241.2; -; Genomic_DNA.
DR   RefSeq; XP_640072.2; XM_634980.2.
DR   AlphaFoldDB; Q54SG7; -.
DR   SMR; Q54SG7; -.
DR   STRING; 44689.DDB0233748; -.
DR   PaxDb; Q54SG7; -.
DR   EnsemblProtists; EAL66241; EAL66241; DDB_G0282763.
DR   GeneID; 8623601; -.
DR   KEGG; ddi:DDB_G0282763; -.
DR   dictyBase; DDB_G0282763; spf27.
DR   eggNOG; KOG3096; Eukaryota.
DR   HOGENOM; CLU_082523_2_1_1; -.
DR   InParanoid; Q54SG7; -.
DR   OMA; SHLSMRH; -.
DR   PhylomeDB; Q54SG7; -.
DR   Reactome; R-DDI-72163; mRNA Splicing - Major Pathway.
DR   PRO; PR:Q54SG7; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR   GO; GO:0000974; C:Prp19 complex; IBA:GO_Central.
DR   GO; GO:0005681; C:spliceosomal complex; ISS:dictyBase.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR   InterPro; IPR008409; SPF27.
DR   PANTHER; PTHR13296; PTHR13296; 1.
DR   Pfam; PF05700; BCAS2; 1.
PE   3: Inferred from homology;
KW   Coiled coil; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW   Spliceosome.
FT   CHAIN           1..226
FT                   /note="Pre-mRNA-splicing factor spf27"
FT                   /id="PRO_0000331259"
FT   COILED          58..222
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   226 AA;  26979 MW;  66B035A895330015 CRC64;
     MNIIIEGAEN IDSLPYVDDS VNENEQELIN KLISDEMSTF TPPDYLAQLP SFIDIDYNNF
     QFLENDFKRM EKEEKMKEFD IGRYKVEPTT TMIKQQLNEK QWNDSLNNAR SQLEHQDIRK
     INLELLQRYG GNSWKLYLSD LEILQKTLKK QLDQKKQQIE EINIQRKLSQ EQTFEKIQQH
     DKKFLELVYK NTEIESACKS IELEIEQLKL KQQLQQQQEN GDLNNK