ID   SPF27_PONAB             Reviewed;         226 AA.
AC   Q5RAX7;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   25-MAY-2022, entry version 56.
DE   RecName: Full=Pre-mRNA-splicing factor SPF27;
DE   AltName: Full=Breast carcinoma-amplified sequence 2 homolog;
GN   Name=BCAS2;
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney;
RG   The German cDNA consortium;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Required for pre-mRNA splicing as component of the activated
CC       spliceosome. Component of the PRP19-CDC5L complex that forms an
CC       integral part of the spliceosome and is required for activating pre-
CC       mRNA splicing. May have a scaffolding role in the spliceosome assembly
CC       as it contacts all other components of the core complex. The PRP19-
CC       CDC5L complex may also play a role in the response to DNA damage (DDR).
CC       {ECO:0000250|UniProtKB:O75934}.
CC   -!- SUBUNIT: Component of the pre-catalytic and catalytic spliceosome
CC       complexes. Component of the postcatalytic spliceosome P complex.
CC       Component of the PRP19-CDC5L splicing complex composed of a core
CC       complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2,
CC       and at least three less stably associated proteins CTNNBL1, CWC15 and
CC       HSPA8. Interacts directly in the complex with PRPF19, CDC5L and PLRG1.
CC       {ECO:0000250|UniProtKB:O75934}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O75934}. Nucleus,
CC       nucleolus {ECO:0000250|UniProtKB:O75934}.
CC   -!- SIMILARITY: Belongs to the SPF27 family. {ECO:0000305}.
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DR   EMBL; CR858884; CAH91083.1; -; mRNA.
DR   RefSeq; NP_001125629.1; NM_001132157.1.
DR   AlphaFoldDB; Q5RAX7; -.
DR   SMR; Q5RAX7; -.
DR   STRING; 9601.ENSPPYP00000001168; -.
DR   GeneID; 100172547; -.
DR   KEGG; pon:100172547; -.
DR   CTD; 10286; -.
DR   eggNOG; KOG3096; Eukaryota.
DR   InParanoid; Q5RAX7; -.
DR   OrthoDB; 1607056at2759; -.
DR   Proteomes; UP000001595; Unplaced.
DR   GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0000974; C:Prp19 complex; ISS:UniProtKB.
DR   GO; GO:0071007; C:U2-type catalytic step 2 spliceosome; ISS:UniProtKB.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR   InterPro; IPR008409; SPF27.
DR   PANTHER; PTHR13296; PTHR13296; 1.
DR   Pfam; PF05700; BCAS2; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; Coiled coil; mRNA processing; mRNA splicing; Nucleus;
KW   Phosphoprotein; Reference proteome; Spliceosome.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250|UniProtKB:O75934"
FT   CHAIN           2..226
FT                   /note="Pre-mRNA-splicing factor SPF27"
FT                   /id="PRO_0000064863"
FT   COILED          139..223
FT                   /evidence="ECO:0000255"
FT   MOD_RES         2
FT                   /note="N-acetylalanine"
FT                   /evidence="ECO:0000250|UniProtKB:O75934"
FT   MOD_RES         94
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:O75934"
SQ   SEQUENCE   226 AA;  26270 MW;  609C4C2A26E4EDE4 CRC64;
     MAGTGLVAGE VVVDALPYFD QGYEAPGVRE AAAALVEEET RRYRPTKNYL SYLTAPDYSA
     FETDIMRNEF ERLAARQPIE LLSMKRYELP APPSGQKKND ITAWQECVNN SMAQLEHQAV
     RIENLELMSQ HGCNAWKVYN ENLVHMIEHA QKELQKLRKH IQDLNWQRKN MQLTAGSKLR
     EMESNWVSLV SKNYEIERTI VQLENEIYQI KQQHGEANKE NIRQDF