ID   SPF27_XENTR             Reviewed;         223 AA.
AC   Q6PBE2; Q28IS3;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=Pre-mRNA-splicing factor SPF27;
DE   AltName: Full=Protein BCAS2 homolog;
GN   Name=bcas2; ORFNames=TNeu112e09.1;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Neurula;
RG   Sanger Xenopus tropicalis EST/cDNA project;
RL   Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Required for pre-mRNA splicing as component of the activated
CC       spliceosome. May have a scaffolding role in the spliceosome assembly as
CC       it contacts all other components of the core complex.
CC       {ECO:0000250|UniProtKB:O75934}.
CC   -!- SUBUNIT: Component of the pre-catalytic and catalytic spliceosome
CC       complexes. Component of the postcatalytic spliceosome P complex.
CC       {ECO:0000250|UniProtKB:O75934}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O75934}.
CC   -!- SIMILARITY: Belongs to the SPF27 family. {ECO:0000305}.
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DR   EMBL; CR760253; CAJ83069.1; -; mRNA.
DR   EMBL; BC059752; AAH59752.1; -; mRNA.
DR   RefSeq; NP_988924.1; NM_203593.1.
DR   AlphaFoldDB; Q6PBE2; -.
DR   SMR; Q6PBE2; -.
DR   STRING; 8364.ENSXETP00000060198; -.
DR   DNASU; 394520; -.
DR   GeneID; 394520; -.
DR   KEGG; xtr:394520; -.
DR   CTD; 10286; -.
DR   Xenbase; XB-GENE-959514; bcas2.
DR   eggNOG; KOG3096; Eukaryota.
DR   InParanoid; Q6PBE2; -.
DR   OrthoDB; 1607056at2759; -.
DR   Reactome; R-XTR-72163; mRNA Splicing - Major Pathway.
DR   Proteomes; UP000008143; Chromosome 6.
DR   Proteomes; UP000790000; Unplaced.
DR   GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0000974; C:Prp19 complex; IBA:GO_Central.
DR   GO; GO:0071007; C:U2-type catalytic step 2 spliceosome; ISS:UniProtKB.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR   InterPro; IPR008409; SPF27.
DR   PANTHER; PTHR13296; PTHR13296; 1.
DR   Pfam; PF05700; BCAS2; 1.
PE   2: Evidence at transcript level;
KW   Coiled coil; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW   Spliceosome.
FT   CHAIN           1..223
FT                   /note="Pre-mRNA-splicing factor SPF27"
FT                   /id="PRO_0000064864"
FT   COILED          139..223
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   223 AA;  25931 MW;  C22A4CC4439D1AFC CRC64;
     MAGTSLVAGD VVVDALPYFD QGYDAQGVRE AAAALVEEET RRYRPTKNYL SYLPTPDYSA
     FETEIMRNEF ERLSSRQPLE LLSMKRYELP APLSGQRNDI TAWQECVNNS MAQLEHQAVR
     IENLELMSQH GCNAWKVYNE NLLHMIDCAQ KDLQKLRKKI QDLNWQRKNS QLTAGARLRE
     MESTWVSLVS KNYEIERAIV QMENEVYQLK ERSGENKENI EDY