SPG16_MOUSE
ID SPG16_MOUSE Reviewed; 639 AA.
AC Q8K450; Q0VBE7; Q8C450; Q9CPU8; Q9D522;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Sperm-associated antigen 16 protein;
DE AltName: Full=Pf20 protein homolog;
GN Name=Spag16; Synonyms=Pf20;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, INTERACTION
RP WITH SPAG6, AND TISSUE SPECIFICITY.
RC STRAIN=CD-1; TISSUE=Testis;
RX PubMed=12391165; DOI=10.1128/mcb.22.22.7993-8004.2002;
RA Zhang Z., Sapiro R., Kapfhamer D., Bucan M., Bray J., Chennathukuzhi V.,
RA McNamara P., Curtis A., Zhang M., Blanchette-Mackie E.J., Strauss J.F. III;
RT "A sperm-associated WD repeat protein orthologous to Chlamydomonas PF20
RT associates with Spag6, the mammalian orthologue of Chlamydomonas PF16.";
RL Mol. Cell. Biol. 22:7993-8004(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 3 AND 4).
RC STRAIN=C57BL/6J; TISSUE=Spinal cord, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION.
RX PubMed=18367677; DOI=10.1095/biolreprod.107.066308;
RA Zhang Z., Shen X., Jones B.H., Xu B., Herr J.C., Strauss J.F. III;
RT "Phosphorylation of mouse sperm axoneme central apparatus protein SPAG16L
RT by a testis-specific kinase, TSSK2.";
RL Biol. Reprod. 79:75-83(2008).
RN [5]
RP FUNCTION, AND INTERACTION WITH STK36.
RX PubMed=19305393; DOI=10.1038/nature07883;
RA Wilson C.W., Nguyen C.T., Chen M.H., Yang J.H., Gacayan R., Huang J.,
RA Chen J.N., Chuang P.T.;
RT "Fused has evolved divergent roles in vertebrate Hedgehog signalling and
RT motile ciliogenesis.";
RL Nature 459:98-102(2009).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [7]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=27682589; DOI=10.1091/mbc.e16-05-0318;
RA Zhang Z., Li W., Zhang Y., Zhang L., Teves M.E., Liu H., Strauss J.F. III,
RA Pazour G.J., Foster J.A., Hess R.A., Zhang Z.;
RT "Intraflagellar transport protein IFT20 is essential for male fertility and
RT spermiogenesis in mice.";
RL Mol. Biol. Cell 0:0-0(2016).
CC -!- FUNCTION: Necessary for sperm flagellar function. Plays a role in
CC motile ciliogenesis. May help to recruit STK36 to the cilium or apical
CC surface of the cell to initiate subsequent steps of construction of the
CC central pair apparatus of motile cilia. {ECO:0000269|PubMed:19305393}.
CC -!- SUBUNIT: Interacts with SPAG6 and STK36. {ECO:0000269|PubMed:12391165,
CC ECO:0000269|PubMed:19305393}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:12391165}.
CC Cytoplasm, cytoskeleton, cilium axoneme {ECO:0000269|PubMed:12391165}.
CC Cytoplasm, cytoskeleton, flagellum axoneme
CC {ECO:0000269|PubMed:12391165}. Cell projection, cilium, flagellum
CC {ECO:0000269|PubMed:12391165, ECO:0000269|PubMed:27682589}.
CC Note=Detected on the sperm flagellum. Detected in the central apparatus
CC of the axoneme. Colocalizes with SPAG6 on microtubules.
CC {ECO:0000269|PubMed:12391165, ECO:0000269|PubMed:27682589}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1; Synonyms=SPAG16L;
CC IsoId=Q8K450-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8K450-2; Sequence=VSP_013507, VSP_013506;
CC Name=3;
CC IsoId=Q8K450-3; Sequence=VSP_013504, VSP_013505;
CC Name=4; Synonyms=SPAG16S;
CC IsoId=Q8K450-4; Sequence=VSP_013503;
CC -!- TISSUE SPECIFICITY: Expressed in testis. {ECO:0000269|PubMed:12391165,
CC ECO:0000269|PubMed:27682589}.
CC -!- PTM: Phosphorylated by TSSK2. {ECO:0000305|PubMed:18367677}.
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DR EMBL; AF490391; AAM97148.1; -; mRNA.
DR EMBL; AK014865; BAB29591.1; -; mRNA.
DR EMBL; AK015884; BAB30016.1; -; mRNA.
DR EMBL; AK016618; BAB30341.1; -; mRNA.
DR EMBL; AK083083; BAC38755.1; -; mRNA.
DR EMBL; BC120668; AAI20669.1; -; mRNA.
DR CCDS; CCDS15027.1; -. [Q8K450-1]
DR CCDS; CCDS69901.1; -. [Q8K450-3]
DR RefSeq; NP_001258462.1; NM_001271533.1. [Q8K450-3]
DR RefSeq; NP_080004.1; NM_025728.4. [Q8K450-4]
DR RefSeq; NP_083436.2; NM_029160.3. [Q8K450-1]
DR AlphaFoldDB; Q8K450; -.
DR SMR; Q8K450; -.
DR BioGRID; 211672; 2.
DR DIP; DIP-59752N; -.
DR IntAct; Q8K450; 1.
DR STRING; 10090.ENSMUSP00000069821; -.
DR PhosphoSitePlus; Q8K450; -.
DR jPOST; Q8K450; -.
DR MaxQB; Q8K450; -.
DR PaxDb; Q8K450; -.
DR PRIDE; Q8K450; -.
DR ProteomicsDB; 257313; -. [Q8K450-1]
DR ProteomicsDB; 257314; -. [Q8K450-2]
DR ProteomicsDB; 257315; -. [Q8K450-3]
DR ProteomicsDB; 257316; -. [Q8K450-4]
DR Antibodypedia; 47659; 218 antibodies from 29 providers.
DR DNASU; 66722; -.
DR Ensembl; ENSMUST00000065425; ENSMUSP00000069821; ENSMUSG00000053153. [Q8K450-1]
DR Ensembl; ENSMUST00000113940; ENSMUSP00000109573; ENSMUSG00000053153. [Q8K450-3]
DR GeneID; 66722; -.
DR KEGG; mmu:66722; -.
DR UCSC; uc007bjg.2; mouse. [Q8K450-2]
DR UCSC; uc007bjh.2; mouse. [Q8K450-1]
DR UCSC; uc007bji.2; mouse. [Q8K450-4]
DR UCSC; uc033fjj.1; mouse. [Q8K450-3]
DR CTD; 79582; -.
DR MGI; MGI:1913972; Spag16.
DR VEuPathDB; HostDB:ENSMUSG00000053153; -.
DR eggNOG; KOG0295; Eukaryota.
DR GeneTree; ENSGT00940000155053; -.
DR HOGENOM; CLU_000288_57_18_1; -.
DR InParanoid; Q8K450; -.
DR OMA; VSDDETW; -.
DR OrthoDB; 1144264at2759; -.
DR PhylomeDB; Q8K450; -.
DR TreeFam; TF351566; -.
DR BioGRID-ORCS; 66722; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Spag16; mouse.
DR PRO; PR:Q8K450; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q8K450; protein.
DR Bgee; ENSMUSG00000053153; Expressed in spermatid and 57 other tissues.
DR Genevisible; Q8K450; MM.
DR GO; GO:1990716; C:axonemal central apparatus; IDA:MGI.
DR GO; GO:0005930; C:axoneme; IDA:UniProtKB.
DR GO; GO:0005576; C:extracellular region; IEA:GOC.
DR GO; GO:0036126; C:sperm flagellum; IDA:UniProtKB.
DR GO; GO:0019901; F:protein kinase binding; IPI:UniProtKB.
DR GO; GO:0035082; P:axoneme assembly; ISO:MGI.
DR GO; GO:0090660; P:cerebrospinal fluid circulation; IGI:MGI.
DR GO; GO:0060271; P:cilium assembly; IDA:UniProtKB.
DR GO; GO:0120197; P:mucociliary clearance; IGI:MGI.
DR GO; GO:0007288; P:sperm axoneme assembly; IMP:MGI.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF00400; WD40; 5.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 3.
DR PROSITE; PS50082; WD_REPEATS_2; 5.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell projection; Cilium;
KW Cilium biogenesis/degradation; Coiled coil; Cytoplasm; Cytoskeleton;
KW Flagellum; Phosphoprotein; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..639
FT /note="Sperm-associated antigen 16 protein"
FT /id="PRO_0000051224"
FT REPEAT 358..397
FT /note="WD 1"
FT REPEAT 400..439
FT /note="WD 2"
FT REPEAT 442..481
FT /note="WD 3"
FT REPEAT 484..523
FT /note="WD 4"
FT REPEAT 526..565
FT /note="WD 5"
FT REPEAT 568..608
FT /note="WD 6"
FT REPEAT 609..639
FT /note="WD 7"
FT REGION 280..333
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 146..218
FT /evidence="ECO:0000255"
FT COMPBIAS 298..328
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..329
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_013503"
FT VAR_SEQ 323..404
FT /note="DSEFPVDMQPDPNVTSCTENVSAAKFDYKLNNIFRLHELPVSCIVMHPCRDY
FT LISCSEDRLWKMVGLPQGNVLLTGSGHTDW -> PSSTESKRVQPSPTESHMVSSRSSS
FT LYPTPSFHQQQSRGNFPTWNKITPQQGSISTREQSTGTLRKRPPKTSCLHQHESNGRF
FT (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_013504"
FT VAR_SEQ 405..639
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_013505"
FT VAR_SEQ 414..415
FT /note="GS -> LT (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_013507"
FT VAR_SEQ 416..639
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_013506"
SQ SEQUENCE 639 AA; 71473 MW; E7A9A55A1180E52F CRC64;
MAAPSGVPPL RVLEELGIGL SPTGEVTEAV TSEGAYYLEQ VTITETSEDE CEYEEIPDDN
FSIPEGEEDL EKAIHIIGEQ ARDIHILEQQ TILPARNVMQ EAIEDFLCNF LIKMGMTRTL
DCFQAEWYEL IQKKGSDFKG LGNVPDVYSQ VMLLETENKN LKKELKHFKQ AAEKAKEDLL
RTQKERDFHR MHHKRIVQEK NKLIADLKGL KLHYASYEPT IRVLHEKHHA LLKEKMLTSL
ERDRAVGKIS GLQATLKNID MGHIQVPVIK GSYESASITR ESGDRAGHSC EKENSSEGPT
QKSLREAREE VGYKSKLKNE KKDSEFPVDM QPDPNVTSCT ENVSAAKFDY KLNNIFRLHE
LPVSCIVMHP CRDYLISCSE DRLWKMVGLP QGNVLLTGSG HTDWLSGCCF HPSGSKLATS
SGDSTIKLWD LNKGECTLTL EGHNHAVWSC TWHSCGDFVA SASLDMTSKI WDVNSERCRY
TLYGHTDSVN SIEFFPFSNI LLTASADKTL SVWDARTGKC EQSLYGHMHS VNDATFTPRG
HIIASCDARG VTKLWDFRKL IPIVSIDVGP SSGNEVNFDQ SGRVLAQASA NGIIHLLDLK
SGQIHKLVGH ESEVHSVVFS HLGENLYSGG SDGTIRLWI
