SPG21_MOUSE
ID SPG21_MOUSE Reviewed; 308 AA.
AC Q9CQC8; Q3U658; Q3UDT7; Q8C1Y7;
DT 21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Maspardin;
DE AltName: Full=Acid cluster protein 33;
DE AltName: Full=Spastic paraplegia 21 autosomal recessive Mast syndrome protein homolog;
GN Name=Spg21;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J, and NOD;
RC TISSUE=Bone marrow, Dendritic cell, Embryo, and Tongue;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP TISSUE SPECIFICITY.
RX PubMed=11113139; DOI=10.1074/jbc.m009270200;
RA Zeitlmann L., Sirim P., Kremmer E., Kolanus W.;
RT "Cloning of ACP33 as a novel intracellular ligand of CD4.";
RL J. Biol. Chem. 276:9123-9132(2001).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Kidney, Lung, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May play a role as a negative regulatory factor in CD4-
CC dependent T-cell activation. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with CD4. Interacts with ALDH16A1. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9CQC8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9CQC8-2; Sequence=VSP_017634;
CC -!- TISSUE SPECIFICITY: Expressed in cell lines FT.1 and in a L cell
CC fibroblast derivative (at protein level).
CC {ECO:0000269|PubMed:11113139}.
CC -!- SIMILARITY: Belongs to the AB hydrolase superfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK009332; BAB26224.1; -; mRNA.
DR EMBL; AK011985; BAB27958.1; -; mRNA.
DR EMBL; AK090017; BAC41047.1; -; mRNA.
DR EMBL; AK149930; BAE29174.1; -; mRNA.
DR EMBL; AK150215; BAE29385.1; -; mRNA.
DR EMBL; AK150581; BAE29675.1; -; mRNA.
DR EMBL; AK153284; BAE31867.1; -; mRNA.
DR EMBL; AK170666; BAE41947.1; -; mRNA.
DR EMBL; BC099434; AAH99434.1; -; mRNA.
DR CCDS; CCDS23293.1; -. [Q9CQC8-1]
DR RefSeq; NP_613050.1; NM_138584.2. [Q9CQC8-1]
DR RefSeq; XP_006511285.1; XM_006511222.3.
DR AlphaFoldDB; Q9CQC8; -.
DR SMR; Q9CQC8; -.
DR BioGRID; 205693; 5.
DR STRING; 10090.ENSMUSP00000034955; -.
DR ESTHER; mouse-SPG21; Maspardin-ACP33-SPG21_like.
DR iPTMnet; Q9CQC8; -.
DR PhosphoSitePlus; Q9CQC8; -.
DR EPD; Q9CQC8; -.
DR jPOST; Q9CQC8; -.
DR MaxQB; Q9CQC8; -.
DR PaxDb; Q9CQC8; -.
DR PeptideAtlas; Q9CQC8; -.
DR PRIDE; Q9CQC8; -.
DR ProteomicsDB; 257317; -. [Q9CQC8-1]
DR ProteomicsDB; 257318; -. [Q9CQC8-2]
DR Antibodypedia; 25890; 110 antibodies from 20 providers.
DR Ensembl; ENSMUST00000034955; ENSMUSP00000034955; ENSMUSG00000032388. [Q9CQC8-1]
DR GeneID; 27965; -.
DR KEGG; mmu:27965; -.
DR UCSC; uc009qdi.1; mouse. [Q9CQC8-1]
DR CTD; 51324; -.
DR MGI; MGI:106403; Spg21.
DR VEuPathDB; HostDB:ENSMUSG00000032388; -.
DR eggNOG; ENOG502QPSD; Eukaryota.
DR GeneTree; ENSGT00390000007857; -.
DR HOGENOM; CLU_052260_0_0_1; -.
DR InParanoid; Q9CQC8; -.
DR OMA; SVDFMVE; -.
DR PhylomeDB; Q9CQC8; -.
DR TreeFam; TF105253; -.
DR BioGRID-ORCS; 27965; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Spg21; mouse.
DR PRO; PR:Q9CQC8; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q9CQC8; protein.
DR Bgee; ENSMUSG00000032388; Expressed in gonadal fat pad and 252 other tissues.
DR ExpressionAtlas; Q9CQC8; baseline and differential.
DR Genevisible; Q9CQC8; MM.
DR GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0030140; C:trans-Golgi network transport vesicle; ISS:UniProtKB.
DR GO; GO:0042609; F:CD4 receptor binding; ISS:UniProtKB.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR000073; AB_hydrolase_1.
DR InterPro; IPR026151; Maspardin.
DR PANTHER; PTHR15913; PTHR15913; 1.
DR Pfam; PF00561; Abhydrolase_1; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Phosphoprotein; Reference proteome.
FT CHAIN 1..308
FT /note="Maspardin"
FT /id="PRO_0000227982"
FT DOMAIN 87..159
FT /note="AB hydrolase-1"
FT /evidence="ECO:0000255"
FT MOD_RES 304
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NZD8"
FT VAR_SEQ 271..308
FT /note="IHLLQFHGTKYAAIDPSVVSAEELEVQKGRLGLSQEEP -> VSPAWTPEHV
FT RFRDPPQDSAP (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_017634"
FT CONFLICT 278
FT /note="G -> V (in Ref. 1; BAE29174)"
FT /evidence="ECO:0000305"
FT CONFLICT 292
FT /note="E -> G (in Ref. 1; BAE31867)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 308 AA; 34952 MW; 9E312FCB0865CBB5 CRC64;
MGEIKVSPDY NWFRSTVPLK KIIVDDDDSK IWSLYDAGPR SIRCPLIFLP PVSGTADVFF
QQILALTGWG YRVIALQYPV YWDHLEFCDG FRKLLDHLQL DKVHLFGASL GGFLAQKFAE
YTHKSPRVHS LILCNAFSDT SIFNQTWTAN SFWLMPAFML KKIVLGNFSS GPVDPMMADA
IDFMVDRLES LGQSELASRL TLNCQNSYVE PHKIRDIPVT IMDVFDQSAL STEAKEEMYK
LYPNARRAHL KTGGNFPYLC RSAEVNLYVQ IHLLQFHGTK YAAIDPSVVS AEELEVQKGR
LGLSQEEP
