ID   SPHR_SYNE7              Reviewed;         257 AA.
AC   P39663; Q31PH7;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   03-AUG-2022, entry version 121.
DE   RecName: Full=Alkaline phosphatase synthesis transcriptional regulatory protein SphR;
GN   Name=sphR; OrderedLocusNames=Synpcc7942_1012;
OS   Synechococcus elongatus (strain PCC 7942 / FACHB-805) (Anacystis nidulans
OS   R2).
OC   Bacteria; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus.
OX   NCBI_TaxID=1140;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8497200; DOI=10.1111/j.1365-2958.1993.tb01205.x;
RA   Aiba H., Nagaya M., Mizuno T.;
RT   "Sensor and regulator proteins from the cyanobacterium Synechococcus
RT   species PCC7942 that belong to the bacterial signal-transduction protein
RT   families: implication in the adaptive response to phosphate limitation.";
RL   Mol. Microbiol. 8:81-91(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 7942 / FACHB-805;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA   Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M.,
RA   Kyrpides N., Lykidis A., Richardson P.;
RT   "Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942.";
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   CHARACTERIZATION.
RX   PubMed=8157591; DOI=10.1128/jb.176.8.2210-2215.1994;
RA   Nagaya M., Aiba H., Mizuno T.;
RT   "The sphR product, a two-component system response regulator protein,
RT   regulates phosphate assimilation in Synechococcus sp. strain PCC 7942 by
RT   binding to two sites upstream from the phoA promoter.";
RL   J. Bacteriol. 176:2210-2215(1994).
CC   -!- FUNCTION: Member of the two-component regulatory system SphR/SphS.
CC       Response regulator. Involved in inducible production of alkaline
CC       phosphatase in response to phosphate limitation as it is directly
CC       involved in the regulation of phoA transcription in response to
CC       phosphate limitation. Binds to two distinct sites upstream from the
CC       phoA promoter.
CC   -!- PTM: Phosphorylated by SphS.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; D13172; BAA02453.1; -; Genomic_DNA.
DR   EMBL; CP000100; ABB57042.1; -; Genomic_DNA.
DR   PIR; S32931; S32931.
DR   RefSeq; WP_011242845.1; NC_007604.1.
DR   AlphaFoldDB; P39663; -.
DR   SMR; P39663; -.
DR   STRING; 1140.Synpcc7942_1012; -.
DR   PRIDE; P39663; -.
DR   EnsemblBacteria; ABB57042; ABB57042; Synpcc7942_1012.
DR   KEGG; syf:Synpcc7942_1012; -.
DR   eggNOG; COG0745; Bacteria.
DR   HOGENOM; CLU_000445_30_4_3; -.
DR   OMA; SYPRRVW; -.
DR   OrthoDB; 817710at2; -.
DR   BioCyc; SYNEL:SYNPCC7942_1012-MON; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006817; P:phosphate ion transport; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00383; trans_reg_C; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR039420; WalR-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR48111; PTHR48111; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   Pfam; PF00486; Trans_reg_C; 1.
DR   SMART; SM00448; REC; 1.
DR   SMART; SM00862; Trans_reg_C; 1.
DR   SUPFAM; SSF46894; SSF46894; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS51755; OMPR_PHOB; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   1: Evidence at protein level;
KW   Activator; DNA-binding; Phosphate transport; Phosphoprotein; Transcription;
KW   Transcription regulation; Transport; Two-component regulatory system.
FT   CHAIN           1..257
FT                   /note="Alkaline phosphatase synthesis transcriptional
FT                   regulatory protein SphR"
FT                   /id="PRO_0000081246"
FT   DOMAIN          25..148
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DNA_BIND        159..257
FT                   /note="OmpR/PhoB-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT   MOD_RES         83
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   257 AA;  29012 MW;  A53FDF257044BE68 CRC64;
     MTSNPLDESM ESLTVVPPAA SPASRILVVE DEAVIRDMVA LVLQQEGFTV DVAADGRTAL
     NYFRSDSPEA GSVTENPDLV VLDLMLPAVN GLDFCRLLRR QGVTVPILML SAKDTETDRV
     VGLEIGADDY LTKPFGTREL VARCRALLRR SQNQPAETPA VLRYEGLKLF PEECRVLLDD
     RELTLSPKEF RLLELFMRHP RRVWSRDQLL EKIWGIDFMG DSKTIDVHIR WLREKIEANP
     SNPSYLLTVR GFGYRLG