ID   SPI1_MYXVL              Reviewed;         369 AA.
AC   P12393;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 2.
DT   29-SEP-2021, entry version 102.
DE   RecName: Full=Serine proteinase inhibitor 1;
DE            Short=Serp-1;
DE            Short=Serpin-1;
GN   Name=SPI-1; Synonyms=SERP1; OrderedLocusNames=m008.1L;
OS   Myxoma virus (strain Lausanne) (MYXV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Leporipoxvirus.
OX   NCBI_TaxID=31530;
OH   NCBI_TaxID=9986; Oryctolagus cuniculus (Rabbit).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2173255; DOI=10.1016/0042-6822(90)90129-f;
RA   Upton C., Macen J.L., Wishart D.S., McFadden G.;
RT   "Myxoma virus and malignant rabbit fibroma virus encode a serpin-like
RT   protein important for virus virulence.";
RL   Virology 179:618-631(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3023828; DOI=10.1128/mcb.6.1.265-276.1986;
RA   Upton C., McFadden G.;
RT   "DNA sequence homology between the terminal inverted repeats of Shope
RT   fibroma virus and an endogenous cellular plasmid species.";
RL   Mol. Cell. Biol. 6:265-276(1986).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3021526; DOI=10.1016/0014-5793(86)80023-0;
RA   Upton C., Carrell R.W., McFadden G.;
RT   "A novel member of the serpin superfamily is encoded on a circular plasmid-
RT   like DNA species isolated from rabbit cells.";
RL   FEBS Lett. 207:115-120(1986).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=10562494; DOI=10.1006/viro.1999.0001;
RA   Cameron C., Hota-Mitchell S., Chen L., Barrett J.W., Cao J.-X.,
RA   Macaulay C., Willer D.O., Evans D.H., McFadden G.;
RT   "The complete DNA sequence of myxoma virus.";
RL   Virology 264:298-318(1999).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 1-369.
RA   Mahon B.P., Lomelino C.L., Ambadapadi S., Yaron J., Keinan S., Kurnikov I.,
RA   Zhang L., Reeves W., Pinard M.A., Macaulay C., McFadden G., Tibbetts S.,
RA   McKenna R., Lucas A.;
RT   "Structure of the Clinically used Myxomaviral Serine Protease Inhibitor 1
RT   (SERP-1).";
RL   Submitted (NOV-2017) to the PDB data bank.
CC   -!- FUNCTION: Important in virulence.
CC   -!- SIMILARITY: Belongs to the serpin family. Poxviruses subfamily.
CC       {ECO:0000305}.
CC   -!- CAUTION: Was originally (PubMed:3023828 and PubMed:3021526) thought to
CC       originate from a plasmid rabbit DNA. The original sample was
CC       contaminated and the gene is derived from myxoma virus. {ECO:0000305}.
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DR   EMBL; M35233; AAA46629.1; -; Genomic_DNA.
DR   EMBL; M12333; AAA81567.1; -; Genomic_DNA.
DR   EMBL; AF170726; AAF15055.1; -; Genomic_DNA.
DR   EMBL; AF170726; AAF14896.1; -; Genomic_DNA.
DR   PIR; A24470; A24470.
DR   PIR; B36418; B36418.
DR   RefSeq; NP_051722.1; NC_001132.2.
DR   RefSeq; NP_051870.1; NC_001132.2.
DR   PDB; 6BJ5; X-ray; 2.50 A; A/B/C=1-315, F/G/H=316-369.
DR   PDBsum; 6BJ5; -.
DR   SMR; P12393; -.
DR   MEROPS; I04.046; -.
DR   GeneID; 932146; -.
DR   GeneID; 932201; -.
DR   KEGG; vg:932146; -.
DR   KEGG; vg:932201; -.
DR   Proteomes; UP000000867; Genome.
DR   GO; GO:0005615; C:extracellular space; IEA:InterPro.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.30.39.10; -; 1.
DR   Gene3D; 3.30.497.10; -; 1.
DR   InterPro; IPR023795; Serpin_CS.
DR   InterPro; IPR023796; Serpin_dom.
DR   InterPro; IPR000215; Serpin_fam.
DR   InterPro; IPR036186; Serpin_sf.
DR   InterPro; IPR042178; Serpin_sf_1.
DR   InterPro; IPR042185; Serpin_sf_2.
DR   PANTHER; PTHR11461; PTHR11461; 1.
DR   Pfam; PF00079; Serpin; 1.
DR   SMART; SM00093; SERPIN; 1.
DR   SUPFAM; SSF56574; SSF56574; 1.
DR   PROSITE; PS00284; SERPIN; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Protease inhibitor; Reference proteome;
KW   Serine protease inhibitor; Virulence.
FT   CHAIN           1..369
FT                   /note="Serine proteinase inhibitor 1"
FT                   /id="PRO_0000094139"
FT   SITE            319..320
FT                   /note="Reactive bond"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        1..16
FT                   /note="MKYLVLVLCLTSCACR -> MFNVVRV (in Ref. 2 and 3)"
FT                   /evidence="ECO:0000305"
FT   HELIX           18..21
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   HELIX           23..26
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   STRAND          33..35
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   HELIX           37..49
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   HELIX           53..58
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   TURN            69..71
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   STRAND          73..81
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   HELIX           88..98
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   STRAND          103..105
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   STRAND          107..111
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   HELIX           112..120
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   TURN            131..135
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   STRAND          142..156
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   HELIX           160..162
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   STRAND          164..170
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   STRAND          173..185
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   STRAND          188..191
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   STRAND          198..205
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   STRAND          208..218
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   HELIX           220..226
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   HELIX           229..238
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   STRAND          243..249
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   STRAND          251..258
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   HELIX           260..265
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   HELIX           270..272
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   TURN            274..276
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   TURN            280..282
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   STRAND          293..302
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   STRAND          305..314
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   STRAND          323..326
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   STRAND          331..337
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   TURN            338..341
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   STRAND          342..349
FT                   /evidence="ECO:0007829|PDB:6BJ5"
FT   STRAND          353..356
FT                   /evidence="ECO:0007829|PDB:6BJ5"
SQ   SEQUENCE   369 AA;  41557 MW;  8DB31CE131C218A0 CRC64;
     MKYLVLVLCL TSCACRDIGL WTFRYVYNES DNVVFSPYGL TSALSVLRIA AGGNTKREID
     VPESVVEDSD AFLALRELFV DASVPLRPEF TAEFSSRFNT SVQRVTFNSE NVKDVINSYV
     KDKTGGDVPR VLDASLDRDT KMLLLSSVRM KTSWRHVFDP SFTTDQPFYS GNVTYKVRMM
     NKIDTLKTET FTLRNVGYSV TELPYKRRQT AMLLVVPDDL GEIVRALDLS LVRFWIRNMR
     KDVCQVVMPK FSVESVLDLR DALQRLGVRD AFDPSRADFG QASPSNDLYV TKVLQTSKIE
     ADERGTTASS DTAITLIPRN ALTAIVANKP FMFLIYHKPT TTVLFMGTIT KGEKVIYDTE
     GRDDVVSSV