ID   SPI1_VACCW              Reviewed;         353 AA.
AC   P15058; Q76ZK3;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   02-JUN-2021, entry version 107.
DE   RecName: Full=Serine proteinase inhibitor 1;
DE            Short=Serp-1;
DE            Short=Serpin-1;
GN   Name=SPI-1; OrderedLocusNames=VACWR205; ORFNames=B22R, B24R, C12L;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2045793; DOI=10.1099/0022-1317-72-6-1349;
RA   Smith G.L., Chan Y.S., Howard S.T.;
RT   "Nucleotide sequence of 42 kbp of vaccinia virus strain WR from near the
RT   right inverted terminal repeat.";
RL   J. Gen. Virol. 72:1349-1376(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2783466; DOI=10.1128/jvi.63.2.600-606.1989;
RA   Kotwal G.J., Moss B.;
RT   "Vaccinia virus encodes two proteins that are structurally related to
RT   members of the plasma serine protease inhibitor superfamily.";
RL   J. Virol. 63:600-606(1989).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2778436; DOI=10.1099/0022-1317-70-9-2333;
RA   Smith G.L., Howard S.T., Chan Y.S.;
RT   "Vaccinia virus encodes a family of genes with homology to serine
RT   proteinase inhibitors.";
RL   J. Gen. Virol. 70:2333-2343(1989).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2849238; DOI=10.1016/s0042-6822(88)90115-8;
RA   Kotwal G.J., Moss B.;
RT   "Analysis of a large cluster of nonessential genes deleted from a vaccinia
RT   virus terminal transposition mutant.";
RL   Virology 167:524-537(1988).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   INDUCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=7831769; DOI=10.1016/s0042-6822(95)80028-x;
RA   Kettle S., Blake N.W., Law K.M., Smith G.L.;
RT   "Vaccinia virus serpins B13R (SPI-2) and B22R (SPI-1) encode M(r) 38.5 and
RT   40K, intracellular polypeptides that do not affect virus virulence in a
RT   murine intranasal model.";
RL   Virology 206:136-147(1995).
RN   [7]
RP   FUNCTION.
RX   PubMed=10502509; DOI=10.1006/viro.1999.9884;
RA   Shisler J.L., Isaacs S.N., Moss B.;
RT   "Vaccinia virus serpin-1 deletion mutant exhibits a host range defect
RT   characterized by low levels of intermediate and late mRNAs.";
RL   Virology 262:298-311(1999).
CC   -!- FUNCTION: Plays a role in mediating viral host range. May act to
CC       inhibit a caspase independent form of apoptosis to allow efficient
CC       virus replication in infected cells. {ECO:0000269|PubMed:10502509}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000269|PubMed:7831769}.
CC   -!- SIMILARITY: Belongs to the serpin family. Poxviruses subfamily.
CC       {ECO:0000305}.
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DR   EMBL; D11079; BAA01852.1; -; Genomic_DNA.
DR   EMBL; M24217; AAA48344.1; -; Genomic_DNA.
DR   EMBL; D00582; BAA00459.1; -; Genomic_DNA.
DR   EMBL; M23701; AAA69589.1; -; Genomic_DNA.
DR   EMBL; AY243312; AAO89484.1; -; Genomic_DNA.
DR   PIR; A30175; WMVZS1.
DR   RefSeq; YP_233087.1; NC_006998.1.
DR   SMR; P15058; -.
DR   MEROPS; I04.028; -.
DR   DNASU; 3707582; -.
DR   GeneID; 3707582; -.
DR   KEGG; vg:3707582; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0005615; C:extracellular space; IEA:InterPro.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.30.39.10; -; 1.
DR   Gene3D; 3.30.497.10; -; 1.
DR   InterPro; IPR023795; Serpin_CS.
DR   InterPro; IPR023796; Serpin_dom.
DR   InterPro; IPR000215; Serpin_fam.
DR   InterPro; IPR036186; Serpin_sf.
DR   InterPro; IPR042178; Serpin_sf_1.
DR   InterPro; IPR042185; Serpin_sf_2.
DR   PANTHER; PTHR11461; PTHR11461; 1.
DR   Pfam; PF00079; Serpin; 1.
DR   SMART; SM00093; SERPIN; 1.
DR   SUPFAM; SSF56574; SSF56574; 1.
DR   PROSITE; PS00284; SERPIN; 1.
PE   2: Evidence at transcript level;
KW   Early protein; Host cytoplasm; Protease inhibitor; Reference proteome;
KW   Serine protease inhibitor.
FT   CHAIN           1..353
FT                   /note="Serine proteinase inhibitor 1"
FT                   /id="PRO_0000094136"
FT   SITE            318..319
FT                   /note="Reactive bond"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   353 AA;  40466 MW;  331A58D188AF34C7 CRC64;
     MDIFKELILK HTDENVLISP VSILSTLSIL NHGAAGSTAE QLSKYIENMN ENTPDDNNDM
     DVDIPYCATL ATANKIYGSD SIEFHASFLQ KIKDDFQTVN FNNANQTKEL INEWVKTMTN
     GKINSLLTSP LSINTRMTVV SAVHFKAMWK YPFSKHLTYT DKFYISKNIV TSVDMMVSTE
     NNLQYVHINE LFGGFSIIDI PYEGNSSMVI ILPDDIEGIY NIEKNITDEK FKKWCGMLST
     KSIDLYMPKF KVEMTEPYNL VPILENLGLT NIFGYYADFS KMCNETITVE KFLHTTFIDV
     NEEYTEASAV TGVFMTNFSM VYRTKVYINH PFMYMIKDNT GRILFIGKYC YPQ