SPI1_YEAST
ID SPI1_YEAST Reviewed; 148 AA.
AC P40092; D3DM57;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Uncharacterized cell wall protein SPI1;
DE AltName: Full=Stationary phase-induced protein 1;
DE Flags: Precursor;
GN Name=SPI1; OrderedLocusNames=YER150W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169868;
RA Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA Botstein D., Davis R.W.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL Nature 387:78-81(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=9613572; DOI=10.1007/s004380050706;
RA Hamada K., Fukuchi S., Arisawa M., Baba M., Kitada K.;
RT "Screening for glycosylphosphatidylinositol (GPI)-dependent cell wall
RT proteins in Saccharomyces cerevisiae.";
RL Mol. Gen. Genet. 258:53-59(1998).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=10383953; DOI=10.1128/jb.181.13.3886-3889.1999;
RA Hamada K., Terashima H., Arisawa M., Yabuki N., Kitada K.;
RT "Amino acid residues in the omega-minus region participate in cellular
RT localization of yeast glycosylphosphatidylinositol-attached proteins.";
RL J. Bacteriol. 181:3886-3889(1999).
RN [6]
RP INDUCTION.
RX PubMed=10641036;
RX DOI=10.1002/(sici)1097-0061(20000130)16:2<139::aid-yea512>3.0.co;2-j;
RA Puig S., Perez-Ortin J.E.;
RT "Stress response and expression patterns in wine fermentations of yeast
RT genes induced at the diauxic shift.";
RL Yeast 16:139-148(2000).
RN [7]
RP INDUCTION.
RX PubMed=11136466; DOI=10.1046/j.1365-2958.2001.02242.x;
RA Kapteyn J.C., ter Riet B., Vink E., Blad S., De Nobel H., Van Den Ende H.,
RA Klis F.M.;
RT "Low external pH induces HOG1-dependent changes in the organization of the
RT Saccharomyces cerevisiae cell wall.";
RL Mol. Microbiol. 39:469-479(2001).
RN [8]
RP FUNCTION, AND INDUCTION.
RX PubMed=16980434; DOI=10.1128/aem.01476-06;
RA Simoes T., Mira N.P., Fernandes A.R., Sa-Correia I.;
RT "The SPI1 gene, encoding a glycosylphosphatidylinositol-anchored cell wall
RT protein, plays a prominent role in the development of yeast resistance to
RT lipophilic weak-acid food preservatives.";
RL Appl. Environ. Microbiol. 72:7168-7175(2006).
CC -!- FUNCTION: Cell wall protein that plays a role in adaptation and
CC resistance to cell wall stress. {ECO:0000269|PubMed:16980434}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall. Membrane; Lipid-anchor, GPI-
CC anchor. Note=Covalently-linked GPI-modified cell wall protein (GPI-
CC CWP).
CC -!- INDUCTION: Induced by transcription factors MSN2 and MSN4 in stationary
CC phase and by transcription factors MSN2, MSN4 and HAA1 upon weak-acid
CC stress. Up-regulated by low pH. {ECO:0000269|PubMed:10641036,
CC ECO:0000269|PubMed:11136466, ECO:0000269|PubMed:16980434}.
CC -!- PTM: The GPI-anchor is attached to the protein in the endoplasmic
CC reticulum and serves to target the protein to the cell surface. There,
CC the glucosamine-inositol phospholipid moiety is cleaved off and the
CC GPI-modified mannoprotein is covalently attached via its lipidless GPI
CC glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.
CC -!- SIMILARITY: Belongs to the SED1 family. {ECO:0000305}.
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DR EMBL; U18917; AAB64677.1; -; Genomic_DNA.
DR EMBL; AY557784; AAS56110.1; -; Genomic_DNA.
DR EMBL; BK006939; DAA07811.1; -; Genomic_DNA.
DR PIR; S50653; S50653.
DR RefSeq; NP_011077.1; NM_001179040.1.
DR AlphaFoldDB; P40092; -.
DR BioGRID; 36899; 36.
DR DIP; DIP-2064N; -.
DR IntAct; P40092; 5.
DR STRING; 4932.YER150W; -.
DR PaxDb; P40092; -.
DR EnsemblFungi; YER150W_mRNA; YER150W; YER150W.
DR GeneID; 856893; -.
DR KEGG; sce:YER150W; -.
DR SGD; S000000952; SPI1.
DR VEuPathDB; FungiDB:YER150W; -.
DR eggNOG; ENOG502S7XK; Eukaryota.
DR HOGENOM; CLU_1760251_0_0_1; -.
DR InParanoid; P40092; -.
DR OMA; XANARAI; -.
DR BioCyc; YEAST:G3O-30311-MON; -.
DR PRO; PR:P40092; -.
DR Proteomes; UP000002311; Chromosome V.
DR RNAct; P40092; protein.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0071944; C:cell periphery; HDA:SGD.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0009277; C:fungal-type cell wall; IDA:SGD.
DR GO; GO:0005199; F:structural constituent of cell wall; IBA:GO_Central.
DR GO; GO:0031505; P:fungal-type cell wall organization; IBA:GO_Central.
DR GO; GO:0010447; P:response to acidic pH; IMP:SGD.
DR InterPro; IPR038843; Sed1/Spi1.
DR PANTHER; PTHR35523; PTHR35523; 1.
PE 2: Evidence at transcript level;
KW Cell wall; Glycoprotein; GPI-anchor; Lipoprotein; Membrane;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..127
FT /note="Uncharacterized cell wall protein SPI1"
FT /id="PRO_0000014317"
FT PROPEP 128..148
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000372448"
FT LIPID 127
FT /note="GPI-anchor amidated asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 41
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 59
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 148 AA; 14996 MW; F65B20225FE2A5ED CRC64;
MLSNAKLLLS LAMASTALGL VSNSSSSVIV VPSSDATIAG NDTATPAPEP SSAAPIFYNS
TATATQYEVV SEFTTYCPEP TTFVTNGATF TVTAPTTLTI TNCPCTIEKP TSETSVSSTH
DVETNSNAAN ARAIPGALGL AGAVMMLL
