SPI2_MOUSE
ID SPI2_MOUSE Reviewed; 405 AA.
AC Q9JK88; Q9D8Z3; Q9D955;
DT 23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Serpin I2;
DE AltName: Full=Serine protease inhibitor 14;
DE Flags: Precursor;
GN Name=Serpini2; Synonyms=Spi14;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Pancreas;
RA Chang W.S., Lin S.C., Wu C.W.;
RT "Isolation and characterization of mouse pancreas-specific serpin gene.";
RL Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Pancreas;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Pancreas;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in pancreas.
CC -!- SIMILARITY: Belongs to the serpin family. {ECO:0000305}.
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DR EMBL; AF251276; AAF65821.1; -; mRNA.
DR EMBL; AK007347; BAB24976.1; -; mRNA.
DR EMBL; AK007510; BAB25079.1; -; mRNA.
DR CCDS; CCDS17413.1; -.
DR RefSeq; NP_080736.2; NM_026460.3.
DR AlphaFoldDB; Q9JK88; -.
DR SMR; Q9JK88; -.
DR BioGRID; 212544; 4.
DR STRING; 10090.ENSMUSP00000046943; -.
DR MEROPS; I04.026; -.
DR GlyGen; Q9JK88; 1 site.
DR PhosphoSitePlus; Q9JK88; -.
DR MaxQB; Q9JK88; -.
DR PaxDb; Q9JK88; -.
DR PRIDE; Q9JK88; -.
DR ProteomicsDB; 257349; -.
DR Antibodypedia; 33688; 270 antibodies from 34 providers.
DR DNASU; 67931; -.
DR Ensembl; ENSMUST00000039047; ENSMUSP00000046943; ENSMUSG00000034139.
DR GeneID; 67931; -.
DR KEGG; mmu:67931; -.
DR UCSC; uc008pna.2; mouse.
DR CTD; 5276; -.
DR MGI; MGI:1915181; Serpini2.
DR VEuPathDB; HostDB:ENSMUSG00000034139; -.
DR eggNOG; KOG2392; Eukaryota.
DR GeneTree; ENSGT00940000161641; -.
DR HOGENOM; CLU_023330_0_4_1; -.
DR InParanoid; Q9JK88; -.
DR OMA; RQTLKFQ; -.
DR OrthoDB; 1124079at2759; -.
DR PhylomeDB; Q9JK88; -.
DR TreeFam; TF343094; -.
DR BioGRID-ORCS; 67931; 1 hit in 72 CRISPR screens.
DR ChiTaRS; Serpini2; mouse.
DR PRO; PR:Q9JK88; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q9JK88; protein.
DR Bgee; ENSMUSG00000034139; Expressed in pyloric antrum and 22 other tissues.
DR ExpressionAtlas; Q9JK88; baseline and differential.
DR Genevisible; Q9JK88; MM.
DR GO; GO:0062023; C:collagen-containing extracellular matrix; HDA:BHF-UCL.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; ISO:MGI.
DR GO; GO:0010951; P:negative regulation of endopeptidase activity; IBA:GO_Central.
DR GO; GO:0030155; P:regulation of cell adhesion; ISO:MGI.
DR Gene3D; 2.30.39.10; -; 1.
DR Gene3D; 3.30.497.10; -; 1.
DR InterPro; IPR023795; Serpin_CS.
DR InterPro; IPR023796; Serpin_dom.
DR InterPro; IPR000215; Serpin_fam.
DR InterPro; IPR036186; Serpin_sf.
DR InterPro; IPR042178; Serpin_sf_1.
DR InterPro; IPR042185; Serpin_sf_2.
DR PANTHER; PTHR11461; PTHR11461; 1.
DR Pfam; PF00079; Serpin; 1.
DR SMART; SM00093; SERPIN; 1.
DR SUPFAM; SSF56574; SSF56574; 1.
DR PROSITE; PS00284; SERPIN; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Protease inhibitor; Reference proteome; Secreted;
KW Serine protease inhibitor; Signal.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..405
FT /note="Serpin I2"
FT /id="PRO_0000032526"
FT SITE 357..358
FT /note="Reactive bond"
FT /evidence="ECO:0000250"
FT CARBOHYD 306
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 5
FT /note="I -> M (in Ref. 2; BAB25079)"
FT /evidence="ECO:0000305"
FT CONFLICT 195
FT /note="K -> T (in Ref. 2; BAB24976)"
FT /evidence="ECO:0000305"
FT CONFLICT 207
FT /note="D -> S (in Ref. 2; BAB24976)"
FT /evidence="ECO:0000305"
FT CONFLICT 212..218
FT /note="KVPMMKA -> RVPTDEV (in Ref. 2; BAB24976)"
FT /evidence="ECO:0000305"
FT CONFLICT 246
FT /note="F -> Y (in Ref. 2; BAB24976)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 405 AA; 45776 MW; 04DF38BE8545DFA8 CRC64;
MNKTILWSFL LFFSGSQTSR ATDQKIADFA VDLYKAISLS HKNNIIFSPL GTTMLLGMVQ
LGAKGKAQQQ ILKTLRMRGT PAGEEFSVLK SLFSAISKKK QEFTFNLASA LYLQEGFIVK
ETYLHSNKEF FQSATKLVDF LDAKTSAQAI STWVESKTDG KIKNMFSEEE FGPLTRLVLV
NAIYFKGDWK QKFRKEDTEM TDFTKKDGST VKVPMMKALL RAQYGYFSQS SMTCQVLELP
YKADEFSLVI ILPTEDTSIE EVENQVTAPH VRRWFSELHE EEVEVSLPRF KIEQKLDLKE
ALYSLNVTEI FSGGCDLSGI TDSSEVYVSR VMQKVFFEIN EDGSEAAAST GINIPAIMSL
TQTQFLANHP FLFILKHIRT ESILFMGKVT DPDIQTTKGR DLDSL
