SPI2_VACCW
ID SPI2_VACCW Reviewed; 345 AA.
AC P15059; Q76ZL0;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1992, sequence version 2.
DT 23-FEB-2022, entry version 113.
DE RecName: Full=Serine proteinase inhibitor 2;
DE Short=Serp-2;
DE Short=Serpin-2;
GN Name=SPI-2; OrderedLocusNames=VACWR195; ORFNames=B13R;
OS Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS WR)).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX NCBI_TaxID=10254;
OH NCBI_TaxID=9913; Bos taurus (Bovine).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2045793; DOI=10.1099/0022-1317-72-6-1349;
RA Smith G.L., Chan Y.S., Howard S.T.;
RT "Nucleotide sequence of 42 kbp of vaccinia virus strain WR from near the
RT right inverted terminal repeat.";
RL J. Gen. Virol. 72:1349-1376(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2783466; DOI=10.1128/jvi.63.2.600-606.1989;
RA Kotwal G.J., Moss B.;
RT "Vaccinia virus encodes two proteins that are structurally related to
RT members of the plasma serine protease inhibitor superfamily.";
RL J. Virol. 63:600-606(1989).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2778436; DOI=10.1099/0022-1317-70-9-2333;
RA Smith G.L., Howard S.T., Chan Y.S.;
RT "Vaccinia virus encodes a family of genes with homology to serine
RT proteinase inhibitors.";
RL J. Gen. Virol. 70:2333-2343(1989).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA Wohlhueter R.;
RT "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT redundancy and an error rate of 0.16/10kb.";
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP INDUCTION.
RX PubMed=7831769; DOI=10.1016/s0042-6822(95)80028-x;
RA Kettle S., Blake N.W., Law K.M., Smith G.L.;
RT "Vaccinia virus serpins B13R (SPI-2) and B22R (SPI-1) encode M(r) 38.5 and
RT 40K, intracellular polypeptides that do not affect virus virulence in a
RT murine intranasal model.";
RL Virology 206:136-147(1995).
RN [6]
RP FUNCTION.
RX PubMed=8709286; DOI=10.1128/jvi.70.9.6479-6485.1996;
RA Dobbelstein M., Shenk T.;
RT "Protection against apoptosis by the vaccinia virus SPI-2 (B13R) gene
RT product.";
RL J. Virol. 70:6479-6485(1996).
RN [7]
RP FUNCTION.
RX PubMed=9049422; DOI=10.1099/0022-1317-78-3-677;
RA Kettle S., Alcami A., Khanna A., Ehret R., Jassoy C., Smith G.L.;
RT "Vaccinia virus serpin B13R (SPI-2) inhibits interleukin-1beta-converting
RT enzyme and protects virus-infected cells from TNF- and Fas-mediated
RT apoptosis, but does not prevent IL-1beta-induced fever.";
RL J. Gen. Virol. 78:677-685(1997).
CC -!- FUNCTION: Inhibits the proteolytic activity of interleukin 1-beta
CC converting enzyme (ICE) and ICE-like enzymes. Can also block apoptosis
CC through host tumor necrosis factor (TNF) receptor.
CC {ECO:0000269|PubMed:8709286, ECO:0000269|PubMed:9049422}.
CC -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000305}.
CC -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC {ECO:0000269|PubMed:7831769}.
CC -!- SIMILARITY: Belongs to the serpin family. Poxviruses subfamily.
CC {ECO:0000305}.
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DR EMBL; D11079; BAA01843.1; -; Genomic_DNA.
DR EMBL; M24218; AAA48346.1; -; Genomic_DNA.
DR EMBL; D00581; BAA00458.1; -; Genomic_DNA.
DR EMBL; AY243312; AAO89474.1; -; Genomic_DNA.
DR PIR; A34035; WMVZW2.
DR RefSeq; YP_233077.1; NC_006998.1.
DR SMR; P15059; -.
DR IntAct; P15059; 36.
DR MEROPS; I04.028; -.
DR DNASU; 3707572; -.
DR GeneID; 3707572; -.
DR KEGG; vg:3707572; -.
DR Reactome; R-HSA-5218900; CASP8 activity is inhibited.
DR Reactome; R-HSA-5357905; Regulation of TNFR1 signaling.
DR Reactome; R-HSA-9686347; Microbial modulation of RIPK1-mediated regulated necrosis.
DR Proteomes; UP000000344; Genome.
DR GO; GO:0005615; C:extracellular space; IEA:InterPro.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR GO; GO:0039650; P:suppression by virus of host cysteine-type endopeptidase activity involved in apoptotic process; IEA:UniProtKB-KW.
DR Gene3D; 2.30.39.10; -; 1.
DR Gene3D; 3.30.497.10; -; 1.
DR InterPro; IPR015557; Serpin_B1.
DR InterPro; IPR023795; Serpin_CS.
DR InterPro; IPR023796; Serpin_dom.
DR InterPro; IPR000215; Serpin_fam.
DR InterPro; IPR036186; Serpin_sf.
DR InterPro; IPR042178; Serpin_sf_1.
DR InterPro; IPR042185; Serpin_sf_2.
DR PANTHER; PTHR11461; PTHR11461; 1.
DR PANTHER; PTHR11461:SF180; PTHR11461:SF180; 1.
DR Pfam; PF00079; Serpin; 1.
DR SMART; SM00093; SERPIN; 1.
DR SUPFAM; SSF56574; SSF56574; 1.
DR PROSITE; PS00284; SERPIN; 1.
PE 2: Evidence at transcript level;
KW Early protein; Host cytoplasm; Host-virus interaction;
KW Inhibition of host caspases by virus;
KW Modulation of host cell apoptosis by virus; Protease inhibitor;
KW Reference proteome; Serine protease inhibitor.
FT CHAIN 1..345
FT /note="Serine proteinase inhibitor 2"
FT /id="PRO_0000094144"
FT SITE 306..307
FT /note="Reactive bond"
FT /evidence="ECO:0000250"
SQ SEQUENCE 345 AA; 38537 MW; 8A067A10B803F5EF CRC64;
MDIFREIASS MKGENVFISP ASISSVLTIL YYGANGSTAE QLSKYVEKEE NMDKVSAQNI
SFKSINKVYG RYSAVFKDSF LRKIGDKFQT VDFTDCRTID AINKCVDIFT EGKINPLLDE
PLSPDTCLLA ISAVYFKAKW LTPFEKEFTS DYPFYVSPTE MVDVSMMSMY GKAFNHASVK
ESFGNFSIIE LPYVGDTSMM VILPDKIDGL ESIEQNLTDT NFKKWCNSLE ATFIDVHIPK
FKVTGSYNLV DTLVKSGLTE VFGSTGDYSN MCNSDVSVDA MIHKTYIDVN EEYTEAAAAT
CALVSDCAST ITNEFCVDHP FIYVIRHVDG KILFVGRYCS PTTNC
